Restriction map for
pF1KE0370
(Linear) (Six-base) MAPSORT of: KIEE0370.seq Check: 7115 from: 1 to: 1599
KIEE0370.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 541 1138 1195 1599
Size: 541 597 57 404
Fragments arranged by size:
597 541 404 57
AatII G_ACGT'C
Cuts at: 0 182 743 1599
Size: 182 561 856
Fragments arranged by size:
856 561 182
AleI CACnn'nnGTG
Cuts at: 0 601 760 1066 1599
Size: 601 159 306 533
Fragments arranged by size:
601 533 306 159
AlwNI CAG_nnn'CTG
Cuts at: 0 291 867 1599
Size: 291 576 732
Fragments arranged by size:
732 576 291
ApaI G_GGCC'C
Cuts at: 0 303 1307 1438 1599
Size: 303 1004 131 161
Fragments arranged by size:
1004 303 161 131
ApoI r'AATT_y
Cuts at: 0 974 1599
Size: 974 625
AvaI C'yCGr_G
Cuts at: 0 922 1599
Size: 922 677
BamHI G'GATC_C
Cuts at: 0 1139 1599
Size: 1139 460
BanI G'GyrC_C
Cuts at: 0 261 1599
Size: 261 1338
BanII G_rGCy'C
Cuts at: 0 146 303 892 1103 1293 1307 1417 1438
Size: 146 157 589 211 190 14 110 21
Cuts at: 1438 1454 1599
Size: 16 145
Fragments arranged by size:
589 211 190 157 146 145 110 21
16 14
BglI GCCn_nnn'nGGC
Cuts at: 0 688 1090 1599
Size: 688 402 509
Fragments arranged by size:
688 509 402
BlpI GC'TnA_GC
Cuts at: 0 1282 1549 1599
Size: 1282 267 50
Fragments arranged by size:
1282 267 50
Bme1580I G_kGCm'C
Cuts at: 0 264 303 1307 1438 1599
Size: 264 39 1004 131 161
Fragments arranged by size:
1004 264 161 131 39
BmgBI CAC'GTC
Cuts at: 0 909 1119 1599
Size: 909 210 480
Fragments arranged by size:
909 480 210
BmrI ACTGGGnnnn_n'
Cuts at: 0 648 1599
Size: 648 951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 698 793 1189 1491 1599
Size: 27 671 95 396 302 108
Fragments arranged by size:
671 396 302 108 95 27
Bpu10I CC'TnA_GC
Cuts at: 0 435 1599
Size: 435 1164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1599
Size: 1276 323
BsaI GGTCTCn'nnnn_
Cuts at: 0 238 1599
Size: 238 1361
BsaAI yAC'GTr
Cuts at: 0 474 1599
Size: 474 1125
BsaHI Gr'CG_yC
Cuts at: 0 179 740 1599
Size: 179 561 859
Fragments arranged by size:
859 561 179
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 671 701 1216 1246 1280 1310 1366 1396
Size: 671 30 515 30 34 30 56 30
Cuts at: 1396 1599
Size: 203
Fragments arranged by size:
671 515 203 56 34 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 741 1444 1599
Size: 741 703 155
Fragments arranged by size:
741 703 155
BseYI C'CCAG_C
Cuts at: 0 257 520 653 1447 1599
Size: 257 263 133 794 152
Fragments arranged by size:
794 263 257 152 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 523 1599
Size: 141 382 1076
Fragments arranged by size:
1076 382 141
BsiEI CG_ry'CG
Cuts at: 0 947 1599
Size: 947 652
BsiHKAI G_wGCw'C
Cuts at: 0 146 751 1103 1599
Size: 146 605 352 496
Fragments arranged by size:
605 496 352 146
BsmI GAATG_Cn'
Cuts at: 0 20 1599
Size: 20 1579
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1335 1599
Size: 1335 264
Bsp1286I G_dGCh'C
Cuts at: 0 146 264 303 751 892 1103 1293 1307
Size: 146 118 39 448 141 211 190 14
Cuts at: 1307 1417 1438 1454 1599
Size: 110 21 16 145
Fragments arranged by size:
448 211 190 146 145 141 118 110
39 21 16 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 541 1138 1195 1599
Size: 541 597 57 404
Fragments arranged by size:
597 541 404 57
BsrDI GCAATG_nn'
Cuts at: 0 1482 1599
Size: 1482 117
BsrFI r'CCGG_y
Cuts at: 0 511 1599
Size: 511 1088
BsrGI T'GTAC_A
Cuts at: 0 1055 1599
Size: 1055 544
BstAPI GCAn_nnn'nTGC
Cuts at: 0 867 1599
Size: 867 732
BstYI r'GATC_y
Cuts at: 0 1139 1599
Size: 1139 460
BtgI C'CryG_G
Cuts at: 0 332 602 695 1599
Size: 332 270 93 904
Fragments arranged by size:
904 332 270 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1251 1599
Size: 1251 348
BtsI GCAGTG_nn'
Cuts at: 0 158 868 1599
Size: 158 710 731
Fragments arranged by size:
731 710 158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1095 1130 1599
Size: 1095 35 469
Fragments arranged by size:
1095 469 35
EaeI y'GGCC_r
Cuts at: 0 944 1394 1599
Size: 944 450 205
Fragments arranged by size:
944 450 205
EagI C'GGCC_G
Cuts at: 0 944 1599
Size: 944 655
EarI CTCTTCn'nnn_
Cuts at: 0 721 820 1045 1599
Size: 721 99 225 554
Fragments arranged by size:
721 554 225 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1139 1323 1519 1599
Size: 1139 184 196 80
Fragments arranged by size:
1139 196 184 80
EcoICRI GAG'CTC
Cuts at: 0 144 1101 1599
Size: 144 957 498
Fragments arranged by size:
957 498 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 698 793 1189 1491 1599
Size: 27 671 95 396 302 108
Fragments arranged by size:
671 396 302 108 95 27
EcoO109I rG'GnC_Cy
Cuts at: 0 299 300 657 965 1178 1304 1435 1599
Size: 299 1 357 308 213 126 131 164
Fragments arranged by size:
357 308 299 213 164 131 126 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 837 1599
Size: 837 762
HaeII r_GCGC'y
Cuts at: 0 369 1599
Size: 369 1230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 791 823 1280 1312 1599
Size: 791 32 457 32 287
Fragments arranged by size:
791 457 287 32 32
MslI CAynn'nnrTG
Cuts at: 0 601 760 1066 1079 1270 1492 1599
Size: 601 159 306 13 191 222 107
Fragments arranged by size:
601 306 222 191 159 107 13
MspA1I CmG'CkG
Cuts at: 0 16 217 291 410 1286 1599
Size: 16 201 74 119 876 313
Fragments arranged by size:
876 313 201 119 74 16
NcoI C'CATG_G
Cuts at: 0 695 1599
Size: 695 904
NspI r_CATG'y
Cuts at: 0 653 1491 1599
Size: 653 838 108
Fragments arranged by size:
838 653 108
PasI CC'CwG_GG
Cuts at: 0 1431 1558 1599
Size: 1431 127 41
Fragments arranged by size:
1431 127 41
PflMI CCAn_nnn'nTGG
Cuts at: 0 515 653 1599
Size: 515 138 946
Fragments arranged by size:
946 515 138
PspOMI G'GGCC_C
Cuts at: 0 299 1303 1434 1599
Size: 299 1004 131 165
Fragments arranged by size:
1004 299 165 131
PvuII CAG'CTG
Cuts at: 0 291 1599
Size: 291 1308
SacI G_AGCT'C
Cuts at: 0 146 1103 1599
Size: 146 957 496
Fragments arranged by size:
957 496 146
SapI GCTCTTCn'nnn_
Cuts at: 0 820 1599
Size: 820 779
ScaI AGT'ACT
Cuts at: 0 154 1599
Size: 154 1445
SfcI C'TryA_G
Cuts at: 0 1418 1599
Size: 1418 181
SmaI CCC'GGG
Cuts at: 0 924 1599
Size: 924 675
SmlI C'TyrA_G
Cuts at: 0 1255 1599
Size: 1255 344
StyI C'CwwG_G
Cuts at: 0 695 1599
Size: 695 904
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 630 1178 1599
Size: 262 368 548 421
Fragments arranged by size:
548 421 368 262
TatI w'GTAC_w
Cuts at: 0 152 1055 1599
Size: 152 903 544
Fragments arranged by size:
903 544 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 458 490 671 703 1366 1398 1599
Size: 458 32 181 32 663 32 201
Fragments arranged by size:
663 458 201 181 32 32 32
XmaI C'CCGG_G
Cuts at: 0 922 1599
Size: 922 677
ZraI GAC'GTC
Cuts at: 0 180 741 1599
Size: 180 561 858
Fragments arranged by size:
858 561 180
Enzymes that do cut:
AarI AatII AleI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BglI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstAPI BstYI BtgI BtgZI BtsI CspCI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I MslI MspA1I NcoI NspI PasI PflMI
PspOMI PvuII SacI SapI ScaI SfcI SmaI SmlI
StyI TaqII TatI TstI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmtI BplI BsaBI BsaWI BsiWI BspEI BspHI
BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI DraI DraIII DrdI EcoNI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmnI