Restriction map for
pF1KE0373
(Linear) (Six-base) MAPSORT of: KIEE0373.seq Check: 530 from: 1 to: 2172
KIEE0373.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 176 1160 2172
Size: 130 46 984 1012
Fragments arranged by size:
1012 984 130 46
AfeI AGC'GCT
Cuts at: 0 298 2172
Size: 298 1874
AflIII A'CryG_T
Cuts at: 0 1345 1921 2172
Size: 1345 576 251
Fragments arranged by size:
1345 576 251
AgeI A'CCGG_T
Cuts at: 0 805 2172
Size: 805 1367
AleI CACnn'nnGTG
Cuts at: 0 2092 2172
Size: 2092 80
ApoI r'AATT_y
Cuts at: 0 126 1031 1087 1128 1219 1307 1333 2172
Size: 126 905 56 41 91 88 26 839
Fragments arranged by size:
905 839 126 91 88 56 41 26
BanII G_rGCy'C
Cuts at: 0 164 2172
Size: 164 2008
BbsI GAAGACnn'nnnn_
Cuts at: 0 623 684 779 2172
Size: 623 61 95 1393
Fragments arranged by size:
1393 623 95 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 79 113 2172
Size: 79 34 2059
Fragments arranged by size:
2059 79 34
BglI GCCn_nnn'nGGC
Cuts at: 0 519 2172
Size: 519 1653
Bme1580I G_kGCm'C
Cuts at: 0 776 2172
Size: 776 1396
BmrI ACTGGGnnnn_n'
Cuts at: 0 559 1591 2172
Size: 559 1032 581
Fragments arranged by size:
1032 581 559
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1315 2172
Size: 1315 857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 277 288 332 1310 1403 1493 2172
Size: 27 250 11 44 978 93 90 679
Fragments arranged by size:
978 679 250 93 90 44 27 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 611 2172
Size: 611 1561
BsaBI GATnn'nnATC
Cuts at: 0 1245 2172
Size: 1245 927
BsaWI w'CCGG_w
Cuts at: 0 224 805 2172
Size: 224 581 1367
Fragments arranged by size:
1367 581 224
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 545 575 1262 1292 1541 1571 2172
Size: 545 30 687 30 249 30 601
Fragments arranged by size:
687 601 545 249 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 722 2172
Size: 265 457 1450
Fragments arranged by size:
1450 457 265
BseYI C'CCAG_C
Cuts at: 0 714 2172
Size: 714 1458
BsiHKAI G_wGCw'C
Cuts at: 0 558 2172
Size: 558 1614
BsmI GAATG_Cn'
Cuts at: 0 1146 2118 2172
Size: 1146 972 54
Fragments arranged by size:
1146 972 54
Bsp1286I G_dGCh'C
Cuts at: 0 164 558 776 2172
Size: 164 394 218 1396
Fragments arranged by size:
1396 394 218 164
BsrDI GCAATG_nn'
Cuts at: 0 568 2172
Size: 568 1604
BsrFI r'CCGG_y
Cuts at: 0 805 1780 2172
Size: 805 975 392
Fragments arranged by size:
975 805 392
BsrGI T'GTAC_A
Cuts at: 0 652 2172
Size: 652 1520
BstYI r'GATC_y
Cuts at: 0 965 2145 2172
Size: 965 1180 27
Fragments arranged by size:
1180 965 27
Bsu36I CC'TnA_GG
Cuts at: 0 1807 2172
Size: 1807 365
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 939 2172
Size: 939 1233
BtsI GCAGTG_nn'
Cuts at: 0 1667 2172
Size: 1667 505
EaeI y'GGCC_r
Cuts at: 0 341 1778 2172
Size: 341 1437 394
Fragments arranged by size:
1437 394 341
EarI CTCTTCn'nnn_
Cuts at: 0 209 412 2172
Size: 209 203 1760
Fragments arranged by size:
1760 209 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 130 176 277 288 332 1160 1310
Size: 27 103 46 101 11 44 828 150
Cuts at: 1310 1403 1493 2172
Size: 93 90 679
Fragments arranged by size:
828 679 150 103 101 93 90 46
44 27 11
EcoO109I rG'GnC_Cy
Cuts at: 0 829 2172
Size: 829 1343
EcoRI G'AATT_C
Cuts at: 0 126 2172
Size: 126 2046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2036 2172
Size: 2036 136
HaeII r_GCGC'y
Cuts at: 0 11 300 2172
Size: 11 289 1872
Fragments arranged by size:
1872 289 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 1541 1573 2042 2074 2172
Size: 241 32 1268 32 469 32 98
Fragments arranged by size:
1268 469 241 98 32 32 32
MfeI C'AATT_G
Cuts at: 0 1509 2172
Size: 1509 663
MslI CAynn'nnrTG
Cuts at: 0 1987 2092 2172
Size: 1987 105 80
Fragments arranged by size:
1987 105 80
MspA1I CmG'CkG
Cuts at: 0 1117 2172
Size: 1117 1055
NdeI CA'TA_TG
Cuts at: 0 1997 2172
Size: 1997 175
NruI TCG'CGA
Cuts at: 0 1524 2172
Size: 1524 648
NspI r_CATG'y
Cuts at: 0 1349 1925 2172
Size: 1349 576 247
Fragments arranged by size:
1349 576 247
PciI A'CATG_T
Cuts at: 0 1345 1921 2172
Size: 1345 576 251
Fragments arranged by size:
1345 576 251
PflMI CCAn_nnn'nTGG
Cuts at: 0 1444 2172
Size: 1444 728
PfoI T'CCnGG_A
Cuts at: 0 1287 1884 2172
Size: 1287 597 288
Fragments arranged by size:
1287 597 288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2132 2164 2172
Size: 2132 32 8
Fragments arranged by size:
2132 32 8
PshAI GACnn'nnGTC
Cuts at: 0 1830 2172
Size: 1830 342
PstI C_TGCA'G
Cuts at: 0 1674 2172
Size: 1674 498
PvuII CAG'CTG
Cuts at: 0 1117 2172
Size: 1117 1055
SexAI A'CCwGG_T
Cuts at: 0 1017 2172
Size: 1017 1155
SfcI C'TryA_G
Cuts at: 0 1670 2172
Size: 1670 502
SspI AAT'ATT
Cuts at: 0 381 1564 2172
Size: 381 1183 608
Fragments arranged by size:
1183 608 381
StyI C'CwwG_G
Cuts at: 0 1841 2172
Size: 1841 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1291 2172
Size: 1291 881
TatI w'GTAC_w
Cuts at: 0 652 2172
Size: 652 1520
XmnI GAAnn'nnTTC
Cuts at: 0 1047 2172
Size: 1047 1125
Enzymes that do cut:
AcuI AfeI AflIII AgeI AleI ApoI BanII BbsI
BcgI BglI Bme1580I BmrI BplI BpmI BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI
BsrFI BsrGI BstYI Bsu36I BtgZI BtsI EaeI EarI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I MfeI MslI
MspA1I NdeI NruI NspI PciI PflMI PfoI PpiI
PshAI PstI PvuII SexAI SfcI SspI StyI TaqII
TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaAI
BsaHI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MluI MmeI MscI NaeI NarI
NcoI NgoMIV NheI NotI NsiI PacI PasI PmeI
PmlI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI