Restriction map for
pF1KE0374
(Linear) (Six-base) MAPSORT of: KIEE0374.seq Check: 4481 from: 1 to: 2340
KIEE0374.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 0 263 2340
Size: 263 2077
AccI GT'mk_AC
Cuts at: 0 77 2340
Size: 77 2263
AclI AA'CG_TT
Cuts at: 0 1946 2340
Size: 1946 394
AfeI AGC'GCT
Cuts at: 0 1751 2340
Size: 1751 589
AflIII A'CryG_T
Cuts at: 0 1453 2340
Size: 1453 887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 151 2340
Size: 151 2189
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 1868 1900 2291 2323 2340
Size: 437 32 1399 32 391 32 17
Fragments arranged by size:
1399 437 391 32 32 32 17
AlwNI CAG_nnn'CTG
Cuts at: 0 334 679 2340
Size: 334 345 1661
Fragments arranged by size:
1661 345 334
ApoI r'AATT_y
Cuts at: 0 1150 2044 2340
Size: 1150 894 296
Fragments arranged by size:
1150 894 296
AseI AT'TA_AT
Cuts at: 0 2272 2340
Size: 2272 68
AvaI C'yCGr_G
Cuts at: 0 40 2340
Size: 40 2300
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1578 1611 2340
Size: 1578 33 729
Fragments arranged by size:
1578 729 33
BanI G'GyrC_C
Cuts at: 0 113 2340
Size: 113 2227
BanII G_rGCy'C
Cuts at: 0 89 2340
Size: 89 2251
BbeI G_GCGC'C
Cuts at: 0 117 2340
Size: 117 2223
BbvCI CC'TCA_GC
Cuts at: 0 1753 2340
Size: 1753 587
BciVI GTATCCnnnnn_n'
Cuts at: 0 498 2340
Size: 498 1842
BglII A'GATC_T
Cuts at: 0 1866 2340
Size: 1866 474
Bme1580I G_kGCm'C
Cuts at: 0 209 573 2340
Size: 209 364 1767
Fragments arranged by size:
1767 364 209
BmrI ACTGGGnnnn_n'
Cuts at: 0 290 883 2340
Size: 290 593 1457
Fragments arranged by size:
1457 593 290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 1891 2128 2340
Size: 1268 623 237 212
Fragments arranged by size:
1268 623 237 212
Bpu10I CC'TnA_GC
Cuts at: 0 1753 2340
Size: 1753 587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1610 2189 2340
Size: 1610 579 151
Fragments arranged by size:
1610 579 151
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 702 2340
Size: 352 350 1638
Fragments arranged by size:
1638 352 350
BsaAI yAC'GTr
Cuts at: 0 1454 2340
Size: 1454 886
BsaBI GATnn'nnATC
Cuts at: 0 800 2340
Size: 800 1540
BsaHI Gr'CG_yC
Cuts at: 0 114 260 2340
Size: 114 146 2080
Fragments arranged by size:
2080 146 114
BsiEI CG_ry'CG
Cuts at: 0 73 1232 2340
Size: 73 1159 1108
Fragments arranged by size:
1159 1108 73
BsiHKAI G_wGCw'C
Cuts at: 0 89 1246 2340
Size: 89 1157 1094
Fragments arranged by size:
1157 1094 89
Bsp1286I G_dGCh'C
Cuts at: 0 89 209 573 1246 2340
Size: 89 120 364 673 1094
Fragments arranged by size:
1094 673 364 120 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 8 2340
Size: 8 2332
BsrBI CCG'CTC
Cuts at: 0 111 2340
Size: 111 2229
BsrDI GCAATG_nn'
Cuts at: 0 504 1154 1677 2119 2340
Size: 504 650 523 442 221
Fragments arranged by size:
650 523 504 442 221
BsrFI r'CCGG_y
Cuts at: 0 72 2340
Size: 72 2268
BssSI C'ACGA_G
Cuts at: 0 106 2340
Size: 106 2234
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1071 1473 2340
Size: 1071 402 867
Fragments arranged by size:
1071 867 402
BstYI r'GATC_y
Cuts at: 0 445 648 1482 1866 2340
Size: 445 203 834 384 474
Fragments arranged by size:
834 474 445 384 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1050 2340
Size: 1050 1290
BtsI GCAGTG_nn'
Cuts at: 0 530 815 1097 1211 2023 2340
Size: 530 285 282 114 812 317
Fragments arranged by size:
812 530 317 285 282 114
ClaI AT'CG_AT
Cuts at: 0 1136 2340
Size: 1136 1204
DraI TTT'AAA
Cuts at: 0 1686 2340
Size: 1686 654
EaeI y'GGCC_r
Cuts at: 0 70 149 290 1205 1229 2340
Size: 70 79 141 915 24 1111
Fragments arranged by size:
1111 915 141 79 70 24
EagI C'GGCC_G
Cuts at: 0 70 1229 2340
Size: 70 1159 1111
Fragments arranged by size:
1159 1111 70
EcoICRI GAG'CTC
Cuts at: 0 87 2340
Size: 87 2253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 1891 2128 2340
Size: 1268 623 237 212
Fragments arranged by size:
1268 623 237 212
EcoRI G'AATT_C
Cuts at: 0 1150 2044 2340
Size: 1150 894 296
Fragments arranged by size:
1150 894 296
EcoRV GAT'ATC
Cuts at: 0 1584 1884 2340
Size: 1584 300 456
Fragments arranged by size:
1584 456 300
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 1362 2281 2317 2340
Size: 198 1164 919 36 23
Fragments arranged by size:
1164 919 198 36 23
HaeII r_GCGC'y
Cuts at: 0 10 117 1753 2340
Size: 10 107 1636 587
Fragments arranged by size:
1636 587 107 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 332 364 869 901 1906 1938 2340
Size: 332 32 505 32 1005 32 402
Fragments arranged by size:
1005 505 402 332 32 32 32
HincII GTy'rAC
Cuts at: 0 1520 2340
Size: 1520 820
HindIII A'AGCT_T
Cuts at: 0 1318 2340
Size: 1318 1022
KasI G'GCGC_C
Cuts at: 0 113 2340
Size: 113 2227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 318 393 567 1689 1992 2340
Size: 2 316 75 174 1122 303 348
Fragments arranged by size:
1122 348 316 303 174 75 2
MscI TGG'CCA
Cuts at: 0 151 292 1207 2340
Size: 151 141 915 1133
Fragments arranged by size:
1133 915 151 141
MslI CAynn'nnrTG
Cuts at: 0 1069 2340
Size: 1069 1271
MspA1I CmG'CkG
Cuts at: 0 50 676 1354 1392 2340
Size: 50 626 678 38 948
Fragments arranged by size:
948 678 626 50 38
NarI GG'CG_CC
Cuts at: 0 114 2340
Size: 114 2226
NdeI CA'TA_TG
Cuts at: 0 312 942 2340
Size: 312 630 1398
Fragments arranged by size:
1398 630 312
PasI CC'CwG_GG
Cuts at: 0 1311 2340
Size: 1311 1029
PflMI CCAn_nnn'nTGG
Cuts at: 0 693 2340
Size: 693 1647
PfoI T'CCnGG_A
Cuts at: 0 1235 2000 2309 2340
Size: 1235 765 309 31
Fragments arranged by size:
1235 765 309 31
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 467 499 2340
Size: 467 32 1841
Fragments arranged by size:
1841 467 32
PshAI GACnn'nnGTC
Cuts at: 0 1525 2340
Size: 1525 815
PstI C_TGCA'G
Cuts at: 0 55 122 163 330 537 2340
Size: 55 67 41 167 207 1803
Fragments arranged by size:
1803 207 167 67 55 41
PvuII CAG'CTG
Cuts at: 0 50 676 1354 1392 2340
Size: 50 626 678 38 948
Fragments arranged by size:
948 678 626 50 38
SacI G_AGCT'C
Cuts at: 0 89 2340
Size: 89 2251
SbfI CC_TGCA'GG
Cuts at: 0 122 2340
Size: 122 2218
ScaI AGT'ACT
Cuts at: 0 279 2340
Size: 279 2061
SfcI C'TryA_G
Cuts at: 0 51 78 118 159 326 533 902 2185
Size: 51 27 40 41 167 207 369 1283
Cuts at: 2185 2340
Size: 155
Fragments arranged by size:
1283 369 207 167 155 51 41 40
27
SfoI GGC'GCC
Cuts at: 0 115 2340
Size: 115 2225
SmlI C'TyrA_G
Cuts at: 0 1625 2204 2340
Size: 1625 579 136
Fragments arranged by size:
1625 579 136
SspI AAT'ATT
Cuts at: 0 789 2340
Size: 789 1551
StyI C'CwwG_G
Cuts at: 0 994 1560 1892 2340
Size: 994 566 332 448
Fragments arranged by size:
994 566 448 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1332 1434 2340
Size: 1332 102 906
Fragments arranged by size:
1332 906 102
TatI w'GTAC_w
Cuts at: 0 44 277 2340
Size: 44 233 2063
Fragments arranged by size:
2063 233 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 617 649 2340
Size: 617 32 1691
Fragments arranged by size:
1691 617 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1071 1243 1308 2340
Size: 1071 172 65 1032
Fragments arranged by size:
1071 1032 172 65
XmnI GAAnn'nnTTC
Cuts at: 0 479 2340
Size: 479 1861
ZraI GAC'GTC
Cuts at: 0 261 2340
Size: 261 2079
Enzymes that do cut:
AatII AccI AclI AfeI AflIII AlfI AloI AlwNI
ApoI AseI AvaI BaeI BanI BanII BbeI BbvCI
BciVI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstXI BstYI BtgZI BtsI ClaI
DraI EaeI EagI EcoICRI Eco57MI EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NarI NdeI PasI PflMI PfoI PpiI PshAI
PstI PvuII SacI SbfI ScaI SfcI SfoI SmlI
SspI StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AcuI AflII AgeI AhdI AleI ApaI
ApaLI AscI AsiSI AvrII BamHI BbsI BcgI BclI
BfrBI BglI BlpI BmgBI BmtI BplI BsaWI BsaXI
BseRI BseYI BsgI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
CspCI DraIII DrdI EarI EciI EcoNI EcoO109I FseI
FspI FspAI HpaI KpnI MfeI MluI NaeI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XbaI XhoI XmaI