Restriction map for
pF1KE0376
(Linear) (Six-base) MAPSORT of: KIEE0376.seq Check: 9237 from: 1 to: 1686
KIEE0376.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
Acc65I G'GTAC_C
Cuts at: 0 197 1686
Size: 197 1489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1388 1451 1686
Size: 757 631 63 235
Fragments arranged by size:
757 631 235 63
AleI CACnn'nnGTG
Cuts at: 0 949 1277 1686
Size: 949 328 409
Fragments arranged by size:
949 409 328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 767 799 1686
Size: 767 32 887
Fragments arranged by size:
887 767 32
ApaLI G'TGCA_C
Cuts at: 0 162 1686
Size: 162 1524
ApoI r'AATT_y
Cuts at: 0 1357 1686
Size: 1357 329
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 624 657 1686
Size: 624 33 1029
Fragments arranged by size:
1029 624 33
BanI G'GyrC_C
Cuts at: 0 197 347 426 1378 1686
Size: 197 150 79 952 308
Fragments arranged by size:
952 308 197 150 79
BanII G_rGCy'C
Cuts at: 0 989 1133 1686
Size: 989 144 553
Fragments arranged by size:
989 553 144
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 1686
Size: 265 1421
BciVI GTATCCnnnnn_n'
Cuts at: 0 1078 1686
Size: 1078 608
BclI T'GATC_A
Cuts at: 0 378 1121 1517 1686
Size: 378 743 396 169
Fragments arranged by size:
743 396 378 169
Bme1580I G_kGCm'C
Cuts at: 0 166 1686
Size: 166 1520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1003 1686
Size: 1003 683
Bpu10I CC'TnA_GC
Cuts at: 0 487 1686
Size: 487 1199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1440 1686
Size: 1440 246
BsaI GGTCTCn'nnnn_
Cuts at: 0 1454 1686
Size: 1454 232
BsaAI yAC'GTr
Cuts at: 0 251 807 1686
Size: 251 556 879
Fragments arranged by size:
879 556 251
BsaHI Gr'CG_yC
Cuts at: 0 1243 1686
Size: 1243 443
BsaWI w'CCGG_w
Cuts at: 0 1088 1686
Size: 1088 598
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 767 797 1686
Size: 767 30 889
Fragments arranged by size:
889 767 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 727 775 1432 1686
Size: 595 132 48 657 254
Fragments arranged by size:
657 595 254 132 48
BseYI C'CCAG_C
Cuts at: 0 137 1008 1111 1686
Size: 137 871 103 575
Fragments arranged by size:
871 575 137 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 787 1276 1686
Size: 460 327 489 410
Fragments arranged by size:
489 460 410 327
BsiEI CG_ry'CG
Cuts at: 0 195 1686
Size: 195 1491
BsiHKAI G_wGCw'C
Cuts at: 0 94 166 1686
Size: 94 72 1520
Fragments arranged by size:
1520 94 72
Bsp1286I G_dGCh'C
Cuts at: 0 94 166 989 1133 1686
Size: 94 72 823 144 553
Fragments arranged by size:
823 553 144 94 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 851 1686
Size: 851 835
BsrBI CCG'CTC
Cuts at: 0 396 1686
Size: 396 1290
BsrFI r'CCGG_y
Cuts at: 0 190 194 1686
Size: 190 4 1492
Fragments arranged by size:
1492 190 4
BssSI C'ACGA_G
Cuts at: 0 1258 1686
Size: 1258 428
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1143 1279 1686
Size: 1143 136 407
Fragments arranged by size:
1143 407 136
BstYI r'GATC_y
Cuts at: 0 503 1025 1181 1686
Size: 503 522 156 505
Fragments arranged by size:
522 505 503 156
BtgI C'CryG_G
Cuts at: 0 315 402 509 577 1609 1676 1686
Size: 315 87 107 68 1032 67 10
Fragments arranged by size:
1032 315 107 87 68 67 10
EaeI y'GGCC_r
Cuts at: 0 192 935 1093 1209 1686
Size: 192 743 158 116 477
Fragments arranged by size:
743 477 192 158 116
EagI C'GGCC_G
Cuts at: 0 192 1686
Size: 192 1494
EarI CTCTTCn'nnn_
Cuts at: 0 1273 1686
Size: 1273 413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1190 1686
Size: 1190 496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1003 1388 1451 1686
Size: 757 246 385 63 235
Fragments arranged by size:
757 385 246 235 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 922 1686
Size: 922 764
EcoO109I rG'GnC_Cy
Cuts at: 0 1631 1686
Size: 1631 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 606 794 1481 1686
Size: 606 188 687 205
Fragments arranged by size:
687 606 205 188
HaeII r_GCGC'y
Cuts at: 0 933 1686
Size: 933 753
KpnI G_GTAC'C
Cuts at: 0 201 1686
Size: 201 1485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1180 1277 1686
Size: 640 540 97 409
Fragments arranged by size:
640 540 409 97
MscI TGG'CCA
Cuts at: 0 937 1686
Size: 937 749
MslI CAynn'nnrTG
Cuts at: 0 673 949 1277 1686
Size: 673 276 328 409
Fragments arranged by size:
673 409 328 276
MspA1I CmG'CkG
Cuts at: 0 151 482 563 1008 1686
Size: 151 331 81 445 678
Fragments arranged by size:
678 445 331 151 81
NcoI C'CATG_G
Cuts at: 0 402 577 1609 1676 1686
Size: 402 175 1032 67 10
Fragments arranged by size:
1032 402 175 67 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 948 1686
Size: 948 738
PfoI T'CCnGG_A
Cuts at: 0 908 1686
Size: 908 778
PmlI CAC'GTG
Cuts at: 0 251 1686
Size: 251 1435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 767 799 1686
Size: 767 32 887
Fragments arranged by size:
887 767 32
PpuMI rG'GwC_Cy
Cuts at: 0 1631 1686
Size: 1631 55
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 1686
Size: 1012 32 642
Fragments arranged by size:
1012 642 32
PstI C_TGCA'G
Cuts at: 0 311 1619 1686
Size: 311 1308 67
Fragments arranged by size:
1308 311 67
PvuII CAG'CTG
Cuts at: 0 151 1008 1686
Size: 151 857 678
Fragments arranged by size:
857 678 151
SfcI C'TryA_G
Cuts at: 0 307 1615 1686
Size: 307 1308 71
Fragments arranged by size:
1308 307 71
SmlI C'TyrA_G
Cuts at: 0 1455 1686
Size: 1455 231
SspI AAT'ATT
Cuts at: 0 102 1686
Size: 102 1584
StyI C'CwwG_G
Cuts at: 0 48 402 577 947 1609 1676 1686
Size: 48 354 175 370 662 67 10
Fragments arranged by size:
662 370 354 175 67 48 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1000 1637 1686
Size: 1000 637 49
Fragments arranged by size:
1000 637 49
TatI w'GTAC_w
Cuts at: 0 953 1686
Size: 953 733
Tth111I GACn'n_nGTC
Cuts at: 0 1161 1686
Size: 1161 525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 945 1140 1686
Size: 945 195 546
Fragments arranged by size:
945 546 195
XmnI GAAnn'nnTTC
Cuts at: 0 779 1401 1475 1686
Size: 779 622 74 211
Fragments arranged by size:
779 622 211 74
Enzymes that do cut:
Acc65I AcuI AleI AloI ApaLI ApoI BaeI BanI
BanII BbsI BciVI BclI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstXI
BstYI BtgI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII KpnI MmeI MscI MslI MspA1I
NcoI PflMI PfoI PmlI PpiI PpuMI PsrI PstI
PvuII SfcI SmlI SspI StyI TaqII TatI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BbvCI BcgI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BsaBI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TstI XbaI XhoI
XmaI ZraI