Restriction map for
pF1KE0378
(Linear) (Six-base) MAPSORT of: KIEE0378.seq Check: 3778 from: 1 to: 1743
KIEE0378.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 1120 1743
Size: 1120 623
AclI AA'CG_TT
Cuts at: 0 1191 1743
Size: 1191 552
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1743
Size: 506 1237
AflIII A'CryG_T
Cuts at: 0 1135 1604 1743
Size: 1135 469 139
Fragments arranged by size:
1135 469 139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 182 185 1743
Size: 182 3 1558
Fragments arranged by size:
1558 182 3
AlwNI CAG_nnn'CTG
Cuts at: 0 1336 1743
Size: 1336 407
ApoI r'AATT_y
Cuts at: 0 16 97 252 337 347 727 1254 1561
Size: 16 81 155 85 10 380 527 307
Cuts at: 1561 1743
Size: 182
Fragments arranged by size:
527 380 307 182 155 85 81 16
10
BanI G'GyrC_C
Cuts at: 0 1434 1743
Size: 1434 309
BclI T'GATC_A
Cuts at: 0 186 1593 1743
Size: 186 1407 150
Fragments arranged by size:
1407 186 150
BglII A'GATC_T
Cuts at: 0 927 1743
Size: 927 816
BmrI ACTGGGnnnn_n'
Cuts at: 0 1385 1511 1743
Size: 1385 126 232
Fragments arranged by size:
1385 232 126
Bpu10I CC'TnA_GC
Cuts at: 0 323 1743
Size: 323 1420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1696 1743
Size: 1696 47
BsaWI w'CCGG_w
Cuts at: 0 620 1743
Size: 620 1123
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 1743
Size: 475 1268
BseYI C'CCAG_C
Cuts at: 0 857 1743
Size: 857 886
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1743
Size: 974 769
BsmI GAATG_Cn'
Cuts at: 0 913 1743
Size: 913 830
BspEI T'CCGG_A
Cuts at: 0 620 1743
Size: 620 1123
BsrDI GCAATG_nn'
Cuts at: 0 665 1507 1743
Size: 665 842 236
Fragments arranged by size:
842 665 236
BstYI r'GATC_y
Cuts at: 0 927 1332 1743
Size: 927 405 411
Fragments arranged by size:
927 411 405
BtsI GCAGTG_nn'
Cuts at: 0 197 1743
Size: 197 1546
DraI TTT'AAA
Cuts at: 0 416 630 683 1743
Size: 416 214 53 1060
Fragments arranged by size:
1060 416 214 53
EarI CTCTTCn'nnn_
Cuts at: 0 461 1743
Size: 461 1282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1743
Size: 506 1237
EcoRI G'AATT_C
Cuts at: 0 252 337 1743
Size: 252 85 1406
Fragments arranged by size:
1406 252 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 654 686 1743
Size: 654 32 1057
Fragments arranged by size:
1057 654 32
HindIII A'AGCT_T
Cuts at: 0 1218 1743
Size: 1218 525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1743
Size: 697 1046
NdeI CA'TA_TG
Cuts at: 0 1473 1743
Size: 1473 270
NspI r_CATG'y
Cuts at: 0 46 80 331 658 1139 1743
Size: 46 34 251 327 481 604
Fragments arranged by size:
604 481 327 251 46 34
PciI A'CATG_T
Cuts at: 0 1135 1743
Size: 1135 608
PstI C_TGCA'G
Cuts at: 0 174 1743
Size: 174 1569
SfcI C'TryA_G
Cuts at: 0 170 1121 1743
Size: 170 951 622
Fragments arranged by size:
951 622 170
SmlI C'TyrA_G
Cuts at: 0 1675 1743
Size: 1675 68
SphI G_CATG'C
Cuts at: 0 46 1743
Size: 46 1697
SwaI ATTT'AAAT
Cuts at: 0 683 1743
Size: 683 1060
XmnI GAAnn'nnTTC
Cuts at: 0 929 1007 1743
Size: 929 78 736
Fragments arranged by size:
929 736 78
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AlwNI ApoI BanI
BclI BglII BmrI Bpu10I BpuEI BsaWI BseRI BseYI
BsgI BsmI BspEI BsrDI BstYI BtsI DraI EarI
Eco57MI EcoRI Hin4I HindIII MmeI NdeI NspI PciI
PstI SfcI SmlI SphI SwaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BpmI
BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI