Restriction map for
pF1KE0379
(Linear) (Six-base) MAPSORT of: KIEE0379.seq Check: 9 from: 1 to: 2382
KIEE0379.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1493 2382
Size: 1493 889
AatII G_ACGT'C
Cuts at: 0 857 1325 2382
Size: 857 468 1057
Fragments arranged by size:
1057 857 468
AccI GT'mk_AC
Cuts at: 0 2057 2382
Size: 2057 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 2314 2382
Size: 481 1833 68
Fragments arranged by size:
1833 481 68
AleI CACnn'nnGTG
Cuts at: 0 127 2382
Size: 127 2255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1324 2382
Size: 1324 1058
AlwNI CAG_nnn'CTG
Cuts at: 0 1117 2382
Size: 1117 1265
ApoI r'AATT_y
Cuts at: 0 391 893 926 1188 2382
Size: 391 502 33 262 1194
Fragments arranged by size:
1194 502 391 262 33
AvaI C'yCGr_G
Cuts at: 0 188 715 1724 1779 2176 2382
Size: 188 527 1009 55 397 206
Fragments arranged by size:
1009 527 397 206 188 55
BanI G'GyrC_C
Cuts at: 0 1588 1719 2172 2215 2382
Size: 1588 131 453 43 167
Fragments arranged by size:
1588 453 167 131 43
BanII G_rGCy'C
Cuts at: 0 734 1829 2382
Size: 734 1095 553
Fragments arranged by size:
1095 734 553
BbeI G_GCGC'C
Cuts at: 0 1592 2382
Size: 1592 790
BlpI GC'TnA_GC
Cuts at: 0 1882 2382
Size: 1882 500
Bme1580I G_kGCm'C
Cuts at: 0 1724 2177 2329 2382
Size: 1724 453 152 53
Fragments arranged by size:
1724 453 152 53
BmgBI CAC'GTC
Cuts at: 0 942 1011 2382
Size: 942 69 1371
Fragments arranged by size:
1371 942 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 427 2382
Size: 427 1955
Bpu10I CC'TnA_GC
Cuts at: 0 725 2382
Size: 725 1657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1798 2382
Size: 1798 584
BsaI GGTCTCn'nnnn_
Cuts at: 0 656 1320 1509 2382
Size: 656 664 189 873
Fragments arranged by size:
873 664 656 189
BsaAI yAC'GTr
Cuts at: 0 1971 2382
Size: 1971 411
BsaBI GATnn'nnATC
Cuts at: 0 682 2382
Size: 682 1700
BsaHI Gr'CG_yC
Cuts at: 0 854 1322 1589 1652 2382
Size: 854 468 267 63 730
Fragments arranged by size:
854 730 468 267 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 1588 1618 1975 2005 2382
Size: 265 30 1293 30 357 30 377
Fragments arranged by size:
1293 377 357 265 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 289 619 2154 2309 2382
Size: 121 168 330 1535 155 73
Fragments arranged by size:
1535 330 168 155 121 73
BseYI C'CCAG_C
Cuts at: 0 1292 1994 2382
Size: 1292 702 388
Fragments arranged by size:
1292 702 388
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1381 1692 1951 2154 2382
Size: 1330 51 311 259 203 228
Fragments arranged by size:
1330 311 259 228 203 51
BsiEI CG_ry'CG
Cuts at: 0 1733 2382
Size: 1733 649
BsiHKAI G_wGCw'C
Cuts at: 0 734 1829 1976 2111 2382
Size: 734 1095 147 135 271
Fragments arranged by size:
1095 734 271 147 135
BsmBI CGTCTCn'nnnn_
Cuts at: 0 792 1886 2382
Size: 792 1094 496
Fragments arranged by size:
1094 792 496
Bsp1286I G_dGCh'C
Cuts at: 0 734 1724 1829 1976 2111 2177 2329 2382
Size: 734 990 105 147 135 66 152 53
Fragments arranged by size:
990 734 152 147 135 105 66 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1302 1493 2012 2382
Size: 1302 191 519 370
Fragments arranged by size:
1302 519 370 191
BsrBI CCG'CTC
Cuts at: 0 2213 2382
Size: 2213 169
BsrFI r'CCGG_y
Cuts at: 0 481 2382
Size: 481 1901
BssHII G'CGCG_C
Cuts at: 0 1214 2382
Size: 1214 1168
BstBI TT'CG_AA
Cuts at: 0 869 2382
Size: 869 1513
BstEII G'GTnAC_C
Cuts at: 0 1449 1749 2382
Size: 1449 300 633
Fragments arranged by size:
1449 633 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1761 2382
Size: 1761 621
BstYI r'GATC_y
Cuts at: 0 683 2382
Size: 683 1699
Bsu36I CC'TnA_GG
Cuts at: 0 1540 2015 2321 2382
Size: 1540 475 306 61
Fragments arranged by size:
1540 475 306 61
BtgI C'CryG_G
Cuts at: 0 1181 1599 1820 2382
Size: 1181 418 221 562
Fragments arranged by size:
1181 562 418 221
ClaI AT'CG_AT
Cuts at: 0 85 2382
Size: 85 2297
EaeI y'GGCC_r
Cuts at: 0 131 1524 1730 1952 2382
Size: 131 1393 206 222 430
Fragments arranged by size:
1393 430 222 206 131
EagI C'GGCC_G
Cuts at: 0 1730 2382
Size: 1730 652
EarI CTCTTCn'nnn_
Cuts at: 0 1500 2098 2382
Size: 1500 598 284
Fragments arranged by size:
1500 598 284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 644 2382
Size: 644 1738
EcoICRI GAG'CTC
Cuts at: 0 732 1827 2382
Size: 732 1095 555
Fragments arranged by size:
1095 732 555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 2314 2382
Size: 481 1833 68
Fragments arranged by size:
1833 481 68
EcoO109I rG'GnC_Cy
Cuts at: 0 147 668 1002 1476 1560 2182 2222 2382
Size: 147 521 334 474 84 622 40 160
Fragments arranged by size:
622 521 474 334 160 147 84 40
EcoRI G'AATT_C
Cuts at: 0 391 2382
Size: 391 1991
FspI TGC'GCA
Cuts at: 0 1867 2382
Size: 1867 515
FspAI rTGC'GCAy
Cuts at: 0 1867 2382
Size: 1867 515
HaeII r_GCGC'y
Cuts at: 0 188 1418 1592 1630 2382
Size: 188 1230 174 38 752
Fragments arranged by size:
1230 752 188 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 634 666 796 828 1099 1131 1414 1446
Size: 634 32 130 32 271 32 283 32
Cuts at: 1446 1588 1620 1762 1794 1975 2007 2382
Size: 142 32 142 32 181 32 375
Fragments arranged by size:
634 375 283 271 181 142 142 130
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 858 2382
Size: 858 1524
KasI G'GCGC_C
Cuts at: 0 1588 2382
Size: 1588 794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 575 1343 2382
Size: 542 33 768 1039
Fragments arranged by size:
1039 768 542 33
MscI TGG'CCA
Cuts at: 0 133 2382
Size: 133 2249
MslI CAynn'nnrTG
Cuts at: 0 127 1306 2382
Size: 127 1179 1076
Fragments arranged by size:
1179 1076 127
MspA1I CmG'CkG
Cuts at: 0 1601 1686 1809 2382
Size: 1601 85 123 573
Fragments arranged by size:
1601 573 123 85
NarI GG'CG_CC
Cuts at: 0 1589 2382
Size: 1589 793
PflMI CCAn_nnn'nTGG
Cuts at: 0 693 2382
Size: 693 1689
PfoI T'CCnGG_A
Cuts at: 0 569 1004 2382
Size: 569 435 1378
Fragments arranged by size:
1378 569 435
PmlI CAC'GTG
Cuts at: 0 1971 2382
Size: 1971 411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2272 2304 2382
Size: 2272 32 78
Fragments arranged by size:
2272 78 32
PpuMI rG'GwC_Cy
Cuts at: 0 147 668 1002 1476 2182 2222 2382
Size: 147 521 334 474 706 40 160
Fragments arranged by size:
706 521 474 334 160 147 40
PstI C_TGCA'G
Cuts at: 0 1568 1691 2382
Size: 1568 123 691
Fragments arranged by size:
1568 691 123
PvuII CAG'CTG
Cuts at: 0 1809 2382
Size: 1809 573
SacI G_AGCT'C
Cuts at: 0 734 1829 2382
Size: 734 1095 553
Fragments arranged by size:
1095 734 553
SacII CC_GC'GG
Cuts at: 0 1602 2382
Size: 1602 780
SanDI GG'GwC_CC
Cuts at: 0 2182 2222 2382
Size: 2182 40 160
Fragments arranged by size:
2182 160 40
SapI GCTCTTCn'nnn_
Cuts at: 0 2098 2382
Size: 2098 284
SexAI A'CCwGG_T
Cuts at: 0 1745 2060 2382
Size: 1745 315 322
Fragments arranged by size:
1745 322 315
SfcI C'TryA_G
Cuts at: 0 956 1564 1687 2382
Size: 956 608 123 695
Fragments arranged by size:
956 695 608 123
SfoI GGC'GCC
Cuts at: 0 1590 2382
Size: 1590 792
SmaI CCC'GGG
Cuts at: 0 1726 2382
Size: 1726 656
SmlI C'TyrA_G
Cuts at: 0 1813 2382
Size: 1813 569
StuI AGG'CCT
Cuts at: 0 971 2382
Size: 971 1411
StyI C'CwwG_G
Cuts at: 0 1279 1754 2382
Size: 1279 475 628
Fragments arranged by size:
1279 628 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1363 1819 2382
Size: 1363 456 563
Fragments arranged by size:
1363 563 456
TatI w'GTAC_w
Cuts at: 0 15 1241 2243 2382
Size: 15 1226 1002 139
Fragments arranged by size:
1226 1002 139 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 146 178 265 297 2382
Size: 146 32 87 32 2085
Fragments arranged by size:
2085 146 87 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 709 1333 2382
Size: 709 624 1049
Fragments arranged by size:
1049 709 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1908 2382
Size: 1908 474
XmaI C'CCGG_G
Cuts at: 0 1724 2382
Size: 1724 658
XmnI GAAnn'nnTTC
Cuts at: 0 86 2368 2382
Size: 86 2282 14
Fragments arranged by size:
2282 86 14
ZraI GAC'GTC
Cuts at: 0 855 1323 2382
Size: 855 468 1059
Fragments arranged by size:
1059 855 468
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BlpI Bme1580I BmgBI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstBI BstEII BstXI BstYI Bsu36I BtgI
ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI PflMI PfoI PmlI PpiI
PpuMI PstI PvuII SacI SacII SanDI SapI SexAI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmrI BmtI BpmI BsaWI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstZ17I BtgZI BtsI CspCI
DraI DraIII DrdI EcoNI EcoRV FalI FseI HindIII
HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XhoI