Restriction map for
pF1KE0381
(Linear) (Six-base) MAPSORT of: KIEE0381.seq Check: 550 from: 1 to: 1539
KIEE0381.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
Acc65I G'GTAC_C
Cuts at: 0 217 236 929 1539
Size: 217 19 693 610
Fragments arranged by size:
693 610 217 19
AfeI AGC'GCT
Cuts at: 0 1069 1539
Size: 1069 470
AhdI GACnn_n'nnGTC
Cuts at: 0 546 1539
Size: 546 993
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1427 1459 1539
Size: 1427 32 80
Fragments arranged by size:
1427 80 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1035 1539
Size: 1035 504
ApaI G_GGCC'C
Cuts at: 0 1310 1539
Size: 1310 229
AvaI C'yCGr_G
Cuts at: 0 118 194 1539
Size: 118 76 1345
Fragments arranged by size:
1345 118 76
BanI G'GyrC_C
Cuts at: 0 217 236 929 1084 1262 1339 1539
Size: 217 19 693 155 178 77 200
Fragments arranged by size:
693 217 200 178 155 77 19
BanII G_rGCy'C
Cuts at: 0 144 500 581 635 1310 1457 1539
Size: 144 356 81 54 675 147 82
Fragments arranged by size:
675 356 147 144 82 81 54
BbeI G_GCGC'C
Cuts at: 0 1266 1343 1539
Size: 1266 77 196
Fragments arranged by size:
1266 196 77
BbvCI CC'TCA_GC
Cuts at: 0 1094 1381 1539
Size: 1094 287 158
Fragments arranged by size:
1094 287 158
BglI GCCn_nnn'nGGC
Cuts at: 0 114 1461 1539
Size: 114 1347 78
Fragments arranged by size:
1347 114 78
BglII A'GATC_T
Cuts at: 0 779 800 1539
Size: 779 21 739
Fragments arranged by size:
779 739 21
Bme1580I G_kGCm'C
Cuts at: 0 1310 1429 1539
Size: 1310 119 110
Fragments arranged by size:
1310 119 110
BmgBI CAC'GTC
Cuts at: 0 684 852 1539
Size: 684 168 687
Fragments arranged by size:
687 684 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 253 1539
Size: 253 1286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 651 1539
Size: 651 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 189 340 442 463 574 622 736
Size: 12 177 151 102 21 111 48 114
Cuts at: 736 775 1174 1291 1539
Size: 39 399 117 248
Fragments arranged by size:
399 248 177 151 117 114 111 102
48 39 21 12
Bpu10I CC'TnA_GC
Cuts at: 0 1094 1381 1539
Size: 1094 287 158
Fragments arranged by size:
1094 287 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1183 1539
Size: 1099 84 356
Fragments arranged by size:
1099 356 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 712 802 1539
Size: 439 273 90 737
Fragments arranged by size:
737 439 273 90
BsaHI Gr'CG_yC
Cuts at: 0 1263 1340 1391 1539
Size: 1263 77 51 148
Fragments arranged by size:
1263 148 77 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 1539
Size: 436 30 1073
Fragments arranged by size:
1073 436 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 643 787 790 961 1171 1539
Size: 643 144 3 171 210 368
Fragments arranged by size:
643 368 210 171 144 3
BseYI C'CCAG_C
Cuts at: 0 964 1064 1230 1510 1525 1539
Size: 964 100 166 280 15 14
Fragments arranged by size:
964 280 166 100 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 581 760 1539
Size: 358 223 179 779
Fragments arranged by size:
779 358 223 179
BsiHKAI G_wGCw'C
Cuts at: 0 144 581 635 1286 1539
Size: 144 437 54 651 253
Fragments arranged by size:
651 437 253 144 54
Bsp1286I G_dGCh'C
Cuts at: 0 144 500 581 635 1286 1310 1429 1457
Size: 144 356 81 54 651 24 119 28
Cuts at: 1457 1539
Size: 82
Fragments arranged by size:
651 356 144 119 82 81 54 28
24
BsrBI CCG'CTC
Cuts at: 0 1481 1539
Size: 1481 58
BsrDI GCAATG_nn'
Cuts at: 0 1410 1539
Size: 1410 129
BsrFI r'CCGG_y
Cuts at: 0 113 938 1539
Size: 113 825 601
Fragments arranged by size:
825 601 113
BssSI C'ACGA_G
Cuts at: 0 736 1539
Size: 736 803
BstEII G'GTnAC_C
Cuts at: 0 81 1539
Size: 81 1458
BstYI r'GATC_y
Cuts at: 0 779 800 1004 1539
Size: 779 21 204 535
Fragments arranged by size:
779 535 204 21
Bsu36I CC'TnA_GG
Cuts at: 0 1396 1539
Size: 1396 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 849 879 1539
Size: 849 30 660
Fragments arranged by size:
849 660 30
BtsI GCAGTG_nn'
Cuts at: 0 101 1539
Size: 101 1438
EarI CTCTTCn'nnn_
Cuts at: 0 620 1274 1539
Size: 620 654 265
Fragments arranged by size:
654 620 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1500 1539
Size: 1500 39
EcoICRI GAG'CTC
Cuts at: 0 142 579 633 1539
Size: 142 437 54 906
Fragments arranged by size:
906 437 142 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 189 340 442 463 574 622 736
Size: 12 177 151 102 21 111 48 114
Cuts at: 736 775 1174 1291 1539
Size: 39 399 117 248
Fragments arranged by size:
399 248 177 151 117 114 111 102
48 39 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 769 1198 1270 1539
Size: 703 66 429 72 269
Fragments arranged by size:
703 429 269 72 66
EcoO109I rG'GnC_Cy
Cuts at: 0 132 191 1539
Size: 132 59 1348
Fragments arranged by size:
1348 132 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 474 1539
Size: 474 1065
HaeII r_GCGC'y
Cuts at: 0 524 1071 1266 1343 1539
Size: 524 547 195 77 196
Fragments arranged by size:
547 524 196 195 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 1539
Size: 532 32 975
Fragments arranged by size:
975 532 32
KasI G'GCGC_C
Cuts at: 0 1262 1339 1539
Size: 1262 77 200
Fragments arranged by size:
1262 200 77
KpnI G_GTAC'C
Cuts at: 0 221 240 933 1539
Size: 221 19 693 606
Fragments arranged by size:
693 606 221 19
MspA1I CmG'CkG
Cuts at: 0 537 964 1032 1539
Size: 537 427 68 507
Fragments arranged by size:
537 507 427 68
NaeI GCC'GGC
Cuts at: 0 115 1539
Size: 115 1424
NarI GG'CG_CC
Cuts at: 0 1263 1340 1539
Size: 1263 77 199
Fragments arranged by size:
1263 199 77
NgoMIV G'CCGG_C
Cuts at: 0 113 1539
Size: 113 1426
PasI CC'CwG_GG
Cuts at: 0 1458 1539
Size: 1458 81
PfoI T'CCnGG_A
Cuts at: 0 419 1007 1539
Size: 419 588 532
Fragments arranged by size:
588 532 419
PshAI GACnn'nnGTC
Cuts at: 0 814 1539
Size: 814 725
PspOMI G'GGCC_C
Cuts at: 0 1306 1539
Size: 1306 233
PstI C_TGCA'G
Cuts at: 0 586 1040 1169 1417 1539
Size: 586 454 129 248 122
Fragments arranged by size:
586 454 248 129 122
PvuII CAG'CTG
Cuts at: 0 537 964 1539
Size: 537 427 575
Fragments arranged by size:
575 537 427
SacI G_AGCT'C
Cuts at: 0 144 581 635 1539
Size: 144 437 54 904
Fragments arranged by size:
904 437 144 54
SapI GCTCTTCn'nnn_
Cuts at: 0 1274 1539
Size: 1274 265
SfcI C'TryA_G
Cuts at: 0 582 1036 1165 1257 1413 1539
Size: 582 454 129 92 156 126
Fragments arranged by size:
582 454 156 129 126 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 114 1539
Size: 114 1425
SfoI GGC'GCC
Cuts at: 0 1264 1341 1539
Size: 1264 77 198
Fragments arranged by size:
1264 198 77
SmaI CCC'GGG
Cuts at: 0 120 196 1539
Size: 120 76 1343
Fragments arranged by size:
1343 120 76
SmlI C'TyrA_G
Cuts at: 0 1078 1198 1539
Size: 1078 120 341
Fragments arranged by size:
1078 341 120
StuI AGG'CCT
Cuts at: 0 925 1539
Size: 925 614
StyI C'CwwG_G
Cuts at: 0 153 1539
Size: 153 1386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 804 1539
Size: 100 704 735
Fragments arranged by size:
735 704 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 436 468 960 992 1539
Size: 436 32 492 32 547
Fragments arranged by size:
547 492 436 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1140 1182 1233 1539
Size: 1140 42 51 306
Fragments arranged by size:
1140 306 51 42
XmaI C'CCGG_G
Cuts at: 0 118 194 1539
Size: 118 76 1345
Fragments arranged by size:
1345 118 76
Enzymes that do cut:
Acc65I AfeI AhdI AloI AlwNI ApaI AvaI BanI
BanII BbeI BbvCI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BssSI
BstEII BstYI Bsu36I BtgZI BtsI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI
MspA1I NaeI NarI NgoMIV PasI PfoI PshAI PspOMI
PstI PvuII SacI SapI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AflIII AgeI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BlpI
BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrGI BssHII BstAPI BstBI BstXI
BstZ17I BtgI ClaI CspCI DraI DraIII DrdI EaeI
EagI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI MscI MslI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI