Restriction map for
pF1KE0383
(Linear) (Six-base) MAPSORT of: KIEE0383.seq Check: 1211 from: 1 to: 3144
KIEE0383.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1480 3144
Size: 1480 1664
AccI GT'mk_AC
Cuts at: 0 600 3144
Size: 600 2544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1966 2060 2827 2863 3144
Size: 1966 94 767 36 281
Fragments arranged by size:
1966 767 281 94 36
AfeI AGC'GCT
Cuts at: 0 709 3144
Size: 709 2435
AflIII A'CryG_T
Cuts at: 0 2003 2301 3144
Size: 2003 298 843
Fragments arranged by size:
2003 843 298
AhdI GACnn_n'nnGTC
Cuts at: 0 810 3144
Size: 810 2334
AleI CACnn'nnGTG
Cuts at: 0 1273 3144
Size: 1273 1871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 985 1048 2077 3144
Size: 985 63 1029 1067
Fragments arranged by size:
1067 1029 985 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1706 1738 3144
Size: 1706 32 1406
Fragments arranged by size:
1706 1406 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1620 2280 2700 2871 3144
Size: 1620 660 420 171 273
Fragments arranged by size:
1620 660 420 273 171
ApoI r'AATT_y
Cuts at: 0 1534 1584 2408 3144
Size: 1534 50 824 736
Fragments arranged by size:
1534 824 736 50
AseI AT'TA_AT
Cuts at: 0 3137 3144
Size: 3137 7
BanI G'GyrC_C
Cuts at: 0 199 2183 2360 3144
Size: 199 1984 177 784
Fragments arranged by size:
1984 784 199 177
BanII G_rGCy'C
Cuts at: 0 191 633 872 3144
Size: 191 442 239 2272
Fragments arranged by size:
2272 442 239 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 1505 2393 3144
Size: 917 588 888 751
Fragments arranged by size:
917 888 751 588
BclI T'GATC_A
Cuts at: 0 992 3144
Size: 992 2152
BglI GCCn_nnn'nGGC
Cuts at: 0 241 3144
Size: 241 2903
BglII A'GATC_T
Cuts at: 0 1416 3144
Size: 1416 1728
BlpI GC'TnA_GC
Cuts at: 0 958 2014 3144
Size: 958 1056 1130
Fragments arranged by size:
1130 1056 958
BmgBI CAC'GTC
Cuts at: 0 9 3144
Size: 9 3135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2204 2705 3030 3144
Size: 2204 501 325 114
Fragments arranged by size:
2204 501 325 114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 916 936 2004 2503 2542 2893 3144
Size: 445 471 20 1068 499 39 351 251
Fragments arranged by size:
1068 499 471 445 351 251 39 20
Bpu10I CC'TnA_GC
Cuts at: 0 2614 3144
Size: 2614 530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1158 1441 1920 2982 3000 3144
Size: 1158 283 479 1062 18 144
Fragments arranged by size:
1158 1062 479 283 144 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 1121 3144
Size: 1121 2023
BsaAI yAC'GTr
Cuts at: 0 1605 3144
Size: 1605 1539
BsaWI w'CCGG_w
Cuts at: 0 1283 3144
Size: 1283 1861
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 1118 1148 2515 2545 3144
Size: 85 30 1003 30 1367 30 599
Fragments arranged by size:
1367 1003 599 85 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 166 571 880 1135 3080 3144
Size: 39 127 405 309 255 1945 64
Fragments arranged by size:
1945 405 309 255 127 64 39
BseYI C'CCAG_C
Cuts at: 0 239 2516 3144
Size: 239 2277 628
Fragments arranged by size:
2277 628 239
BsiEI CG_ry'CG
Cuts at: 0 1709 2174 3144
Size: 1709 465 970
Fragments arranged by size:
1709 970 465
BsiHKAI G_wGCw'C
Cuts at: 0 191 633 872 3144
Size: 191 442 239 2272
Fragments arranged by size:
2272 442 239 191
BsmI GAATG_Cn'
Cuts at: 0 2670 2976 3144
Size: 2670 306 168
Fragments arranged by size:
2670 306 168
Bsp1286I G_dGCh'C
Cuts at: 0 191 633 872 3144
Size: 191 442 239 2272
Fragments arranged by size:
2272 442 239 191
BspHI T'CATG_A
Cuts at: 0 995 1997 3144
Size: 995 1002 1147
Fragments arranged by size:
1147 1002 995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1480 3144
Size: 1480 1664
BsrDI GCAATG_nn'
Cuts at: 0 729 2061 3144
Size: 729 1332 1083
Fragments arranged by size:
1332 1083 729
BsrGI T'GTAC_A
Cuts at: 0 128 543 2857 3144
Size: 128 415 2314 287
Fragments arranged by size:
2314 415 287 128
BssSI C'ACGA_G
Cuts at: 0 553 808 1519 3144
Size: 553 255 711 1625
Fragments arranged by size:
1625 711 553 255
BstAPI GCAn_nnn'nTGC
Cuts at: 0 927 3144
Size: 927 2217
BstBI TT'CG_AA
Cuts at: 0 304 3144
Size: 304 2840
BstXI CCAn_nnnn'nTGG
Cuts at: 0 615 3144
Size: 615 2529
BstYI r'GATC_y
Cuts at: 0 796 1334 1416 2350 2867 3144
Size: 796 538 82 934 517 277
Fragments arranged by size:
934 796 538 517 277 82
Bsu36I CC'TnA_GG
Cuts at: 0 2215 3144
Size: 2215 929
BtgI C'CryG_G
Cuts at: 0 614 1148 1709 2915 3144
Size: 614 534 561 1206 229
Fragments arranged by size:
1206 614 561 534 229
BtsI GCAGTG_nn'
Cuts at: 0 1815 2377 3144
Size: 1815 562 767
Fragments arranged by size:
1815 767 562
DrdI GACnn_nn'nnGTC
Cuts at: 0 1507 3144
Size: 1507 1637
EaeI y'GGCC_r
Cuts at: 0 617 656 1370 1706 2171 2396 3144
Size: 617 39 714 336 465 225 748
Fragments arranged by size:
748 714 617 465 336 225 39
EagI C'GGCC_G
Cuts at: 0 1706 2171 3144
Size: 1706 465 973
Fragments arranged by size:
1706 973 465
EarI CTCTTCn'nnn_
Cuts at: 0 1171 3022 3029 3144
Size: 1171 1851 7 115
Fragments arranged by size:
1851 1171 115 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 62 3144
Size: 62 3082
EcoICRI GAG'CTC
Cuts at: 0 189 631 870 3144
Size: 189 442 239 2274
Fragments arranged by size:
2274 442 239 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 916 936 1966 2004 2060 2503 2542
Size: 445 471 20 1030 38 56 443 39
Cuts at: 2542 2827 2863 2893 3144
Size: 285 36 30 251
Fragments arranged by size:
1030 471 445 443 285 251 56 39
38 36 30 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2586 3144
Size: 2586 558
EcoO109I rG'GnC_Cy
Cuts at: 0 804 900 1182 1815 2146 2838 3144
Size: 804 96 282 633 331 692 306
Fragments arranged by size:
804 692 633 331 306 282 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 192 1435 2736 3144
Size: 192 1243 1301 408
Fragments arranged by size:
1301 1243 408 192
HaeII r_GCGC'y
Cuts at: 0 711 1620 2047 3144
Size: 711 909 427 1097
Fragments arranged by size:
1097 909 711 427
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 796 828 859 891 1118 1150 1614 1646
Size: 796 32 31 32 227 32 464 32
Cuts at: 1646 2071 2103 2515 2547 2734 2766 3023 3055
Size: 425 32 412 32 187 32 257 32
Cuts at: 3055 3112 3144
Size: 57 32
Fragments arranged by size:
796 464 425 412 257 227 187 57
32 32 32 32 32 32 32 32
32 31
HindIII A'AGCT_T
Cuts at: 0 2329 3144
Size: 2329 815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2363 3144
Size: 2363 781
MscI TGG'CCA
Cuts at: 0 619 658 2398 3144
Size: 619 39 1740 746
Fragments arranged by size:
1740 746 619 39
MslI CAynn'nnrTG
Cuts at: 0 1254 1273 1725 1787 2002 3144
Size: 1254 19 452 62 215 1142
Fragments arranged by size:
1254 1142 452 215 62 19
MspA1I CmG'CkG
Cuts at: 0 239 2520 3144
Size: 239 2281 624
Fragments arranged by size:
2281 624 239
NcoI C'CATG_G
Cuts at: 0 1148 2915 3144
Size: 1148 1767 229
Fragments arranged by size:
1767 1148 229
NdeI CA'TA_TG
Cuts at: 0 490 3144
Size: 490 2654
NotI GC'GGCC_GC
Cuts at: 0 2171 3144
Size: 2171 973
NspI r_CATG'y
Cuts at: 0 1025 1386 2305 3144
Size: 1025 361 919 839
Fragments arranged by size:
1025 919 839 361
PasI CC'CwG_GG
Cuts at: 0 594 3144
Size: 594 2550
PciI A'CATG_T
Cuts at: 0 2301 3144
Size: 2301 843
PflMI CCAn_nnn'nTGG
Cuts at: 0 215 2916 3144
Size: 215 2701 228
Fragments arranged by size:
2701 228 215
PfoI T'CCnGG_A
Cuts at: 0 1847 2870 3144
Size: 1847 1023 274
Fragments arranged by size:
1847 1023 274
PmlI CAC'GTG
Cuts at: 0 1605 3144
Size: 1605 1539
PpuMI rG'GwC_Cy
Cuts at: 0 804 900 1182 1815 3144
Size: 804 96 282 633 1329
Fragments arranged by size:
1329 804 633 282 96
PshAI GACnn'nnGTC
Cuts at: 0 1447 3144
Size: 1447 1697
PstI C_TGCA'G
Cuts at: 0 2372 2696 3144
Size: 2372 324 448
Fragments arranged by size:
2372 448 324
PvuII CAG'CTG
Cuts at: 0 239 2520 3144
Size: 239 2281 624
Fragments arranged by size:
2281 624 239
SacI G_AGCT'C
Cuts at: 0 191 633 872 3144
Size: 191 442 239 2272
Fragments arranged by size:
2272 442 239 191
SanDI GG'GwC_CC
Cuts at: 0 1182 1815 3144
Size: 1182 633 1329
Fragments arranged by size:
1329 1182 633
ScaI AGT'ACT
Cuts at: 0 195 562 3144
Size: 195 367 2582
Fragments arranged by size:
2582 367 195
SexAI A'CCwGG_T
Cuts at: 0 221 3144
Size: 221 2923
SfcI C'TryA_G
Cuts at: 0 2368 2692 3144
Size: 2368 324 452
Fragments arranged by size:
2368 452 324
SmlI C'TyrA_G
Cuts at: 0 1137 1420 1899 2961 3015 3144
Size: 1137 283 479 1062 54 129
Fragments arranged by size:
1137 1062 479 283 129 54
SspI AAT'ATT
Cuts at: 0 1960 3144
Size: 1960 1184
StuI AGG'CCT
Cuts at: 0 1696 3010 3144
Size: 1696 1314 134
Fragments arranged by size:
1696 1314 134
StyI C'CwwG_G
Cuts at: 0 380 537 1148 1461 2915 3144
Size: 380 157 611 313 1454 229
Fragments arranged by size:
1454 611 380 313 229 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1389 3110 3144
Size: 1389 1721 34
Fragments arranged by size:
1721 1389 34
TatI w'GTAC_w
Cuts at: 0 128 193 543 560 2857 3144
Size: 128 65 350 17 2297 287
Fragments arranged by size:
2297 350 287 128 65 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 18 50 1345 1377 3144
Size: 18 32 1295 32 1767
Fragments arranged by size:
1767 1295 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 0 223 1132 3144
Size: 223 909 2012
Fragments arranged by size:
2012 909 223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1105 2139 3144
Size: 1105 1034 1005
Fragments arranged by size:
1105 1034 1005
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApoI AseI BanI BanII BbsI BclI
BglI BglII BlpI BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NdeI NotI NspI PasI PciI PflMI PfoI PmlI
PpuMI PshAI PstI PvuII SacI SanDI ScaI SexAI
SfcI SmlI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI Bme1580I BmrI BmtI BsaBI BsaHI BsgI
BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NruI NsiI PacI PmeI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI XbaI XhoI XmaI
XmnI ZraI