Restriction map for
pF1KE0387
(Linear) (Six-base) MAPSORT of: KIEE0387.seq Check: 3653 from: 1 to: 1809
KIEE0387.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1738 1809
Size: 1738 71
AccI GT'mk_AC
Cuts at: 0 171 548 1809
Size: 171 377 1261
Fragments arranged by size:
1261 377 171
AclI AA'CG_TT
Cuts at: 0 184 1809
Size: 184 1625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 540 595 1809
Size: 232 308 55 1214
Fragments arranged by size:
1214 308 232 55
AfeI AGC'GCT
Cuts at: 0 415 1809
Size: 415 1394
AflII C'TTAA_G
Cuts at: 0 1168 1809
Size: 1168 641
AlwNI CAG_nnn'CTG
Cuts at: 0 210 1761 1809
Size: 210 1551 48
Fragments arranged by size:
1551 210 48
ApoI r'AATT_y
Cuts at: 0 1342 1795 1809
Size: 1342 453 14
Fragments arranged by size:
1342 453 14
BamHI G'GATC_C
Cuts at: 0 887 1809
Size: 887 922
BanI G'GyrC_C
Cuts at: 0 112 141 392 1809
Size: 112 29 251 1417
Fragments arranged by size:
1417 251 112 29
BanII G_rGCy'C
Cuts at: 0 163 1364 1441 1809
Size: 163 1201 77 368
Fragments arranged by size:
1201 368 163 77
BbeI G_GCGC'C
Cuts at: 0 145 1809
Size: 145 1664
BbsI GAAGACnn'nnnn_
Cuts at: 0 285 596 1809
Size: 285 311 1213
Fragments arranged by size:
1213 311 285
BglI GCCn_nnn'nGGC
Cuts at: 0 679 1809
Size: 679 1130
BglII A'GATC_T
Cuts at: 0 1019 1809
Size: 1019 790
BlpI GC'TnA_GC
Cuts at: 0 251 1809
Size: 251 1558
Bme1580I G_kGCm'C
Cuts at: 0 790 1130 1402 1809
Size: 790 340 272 407
Fragments arranged by size:
790 407 340 272
BmrI ACTGGGnnnn_n'
Cuts at: 0 605 695 876 1809
Size: 605 90 181 933
Fragments arranged by size:
933 605 181 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 266 1809
Size: 266 1543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 503 884 1080 1539 1559 1809
Size: 442 61 381 196 459 20 250
Fragments arranged by size:
459 442 381 250 196 61 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 1535 1809
Size: 1535 274
BsaBI GATnn'nnATC
Cuts at: 0 892 1809
Size: 892 917
BsaHI Gr'CG_yC
Cuts at: 0 142 1809
Size: 142 1667
BsaWI w'CCGG_w
Cuts at: 0 239 1809
Size: 239 1570
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 1809
Size: 472 1337
BseYI C'CCAG_C
Cuts at: 0 666 922 1249 1547 1751 1809
Size: 666 256 327 298 204 58
Fragments arranged by size:
666 327 298 256 204 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1433 1809
Size: 1433 376
BsiEI CG_ry'CG
Cuts at: 0 1145 1809
Size: 1145 664
BsiHKAI G_wGCw'C
Cuts at: 0 506 572 1809
Size: 506 66 1237
Fragments arranged by size:
1237 506 66
BsmI GAATG_Cn'
Cuts at: 0 668 1809
Size: 668 1141
Bsp1286I G_dGCh'C
Cuts at: 0 163 506 572 790 1130 1364 1402 1441
Size: 163 343 66 218 340 234 38 39
Cuts at: 1441 1809
Size: 368
Fragments arranged by size:
368 343 340 234 218 163 66 39
38
BspEI T'CCGG_A
Cuts at: 0 239 1809
Size: 239 1570
BspHI T'CATG_A
Cuts at: 0 1721 1809
Size: 1721 88
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1343 1491 1738 1809
Size: 1343 148 247 71
Fragments arranged by size:
1343 247 148 71
BsrBI CCG'CTC
Cuts at: 0 99 1809
Size: 99 1710
BsrDI GCAATG_nn'
Cuts at: 0 758 1809
Size: 758 1051
BsrFI r'CCGG_y
Cuts at: 0 144 952 1223 1809
Size: 144 808 271 586
Fragments arranged by size:
808 586 271 144
BstAPI GCAn_nnn'nTGC
Cuts at: 0 844 1694 1809
Size: 844 850 115
Fragments arranged by size:
850 844 115
BstBI TT'CG_AA
Cuts at: 0 627 1809
Size: 627 1182
BstYI r'GATC_y
Cuts at: 0 887 1019 1385 1715 1809
Size: 887 132 366 330 94
Fragments arranged by size:
887 366 330 132 94
BtgI C'CryG_G
Cuts at: 0 968 1355 1473 1809
Size: 968 387 118 336
Fragments arranged by size:
968 387 336 118
BtsI GCAGTG_nn'
Cuts at: 0 1704 1762 1809
Size: 1704 58 47
Fragments arranged by size:
1704 58 47
DraIII CAC_nnn'GTG
Cuts at: 0 501 718 1809
Size: 501 217 1091
Fragments arranged by size:
1091 501 217
EaeI y'GGCC_r
Cuts at: 0 950 1142 1178 1809
Size: 950 192 36 631
Fragments arranged by size:
950 631 192 36
EagI C'GGCC_G
Cuts at: 0 1142 1809
Size: 1142 667
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 21 1809
Size: 21 1788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 442 503 540 595 884 1080 1539
Size: 232 210 61 37 55 289 196 459
Cuts at: 1539 1559 1809
Size: 20 250
Fragments arranged by size:
459 289 250 232 210 196 61 55
37 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1095 1809
Size: 1095 714
EcoO109I rG'GnC_Cy
Cuts at: 0 656 671 1137 1332 1430 1809
Size: 656 15 466 195 98 379
Fragments arranged by size:
656 466 379 195 98 15
EcoRV GAT'ATC
Cuts at: 0 387 1809
Size: 387 1422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1584 1809
Size: 1584 225
HaeII r_GCGC'y
Cuts at: 0 40 88 145 417 1224 1809
Size: 40 48 57 272 807 585
Fragments arranged by size:
807 585 272 57 48 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 297 326 329 346 358 378 457 489
Size: 297 29 3 17 12 20 79 32
Cuts at: 489 1514 1546 1809
Size: 1025 32 263
Fragments arranged by size:
1025 297 263 79 32 32 29 20
17 12 3
HincII GTy'rAC
Cuts at: 0 172 549 732 1809
Size: 172 377 183 1077
Fragments arranged by size:
1077 377 183 172
KasI G'GCGC_C
Cuts at: 0 141 1809
Size: 141 1668
MspA1I CmG'CkG
Cuts at: 0 1249 1635 1685 1809
Size: 1249 386 50 124
Fragments arranged by size:
1249 386 124 50
NaeI GCC'GGC
Cuts at: 0 146 1225 1809
Size: 146 1079 584
Fragments arranged by size:
1079 584 146
NarI GG'CG_CC
Cuts at: 0 142 1809
Size: 142 1667
NgoMIV G'CCGG_C
Cuts at: 0 144 1223 1809
Size: 144 1079 586
Fragments arranged by size:
1079 586 144
NspI r_CATG'y
Cuts at: 0 1500 1809
Size: 1500 309
PasI CC'CwG_GG
Cuts at: 0 118 1809
Size: 118 1691
PpuMI rG'GwC_Cy
Cuts at: 0 656 1137 1332 1809
Size: 656 481 195 477
Fragments arranged by size:
656 481 477 195
PstI C_TGCA'G
Cuts at: 0 29 911 1374 1699 1809
Size: 29 882 463 325 110
Fragments arranged by size:
882 463 325 110 29
PvuII CAG'CTG
Cuts at: 0 1635 1685 1809
Size: 1635 50 124
Fragments arranged by size:
1635 124 50
SalI G'TCGA_C
Cuts at: 0 170 547 1809
Size: 170 377 1262
Fragments arranged by size:
1262 377 170
SbfI CC_TGCA'GG
Cuts at: 0 29 1809
Size: 29 1780
SfcI C'TryA_G
Cuts at: 0 25 907 1113 1370 1695 1809
Size: 25 882 206 257 325 114
Fragments arranged by size:
882 325 257 206 114 25
SfoI GGC'GCC
Cuts at: 0 143 1809
Size: 143 1666
SgrAI Cr'CCGG_yG
Cuts at: 0 144 1809
Size: 144 1665
SmlI C'TyrA_G
Cuts at: 0 1168 1809
Size: 1168 641
StyI C'CwwG_G
Cuts at: 0 1433 1809
Size: 1433 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 310 410 1765 1809
Size: 310 100 1355 44
Fragments arranged by size:
1355 310 100 44
TatI w'GTAC_w
Cuts at: 0 540 875 1208 1809
Size: 540 335 333 601
Fragments arranged by size:
601 540 335 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 964 996 1809
Size: 964 32 813
Fragments arranged by size:
964 813 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1246 1675 1809
Size: 1246 429 134
Fragments arranged by size:
1246 429 134
XmnI GAAnn'nnTTC
Cuts at: 0 982 1809
Size: 982 827
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AlwNI ApoI
BamHI BanI BanII BbeI BbsI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BsaI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstYI
BtgI BtsI DraIII EaeI EagI EciI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII KasI MspA1I
NaeI NarI NgoMIV NspI PasI PpuMI PstI PvuII
SalI SbfI SfcI SfoI SgrAI SmlI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AflIII AgeI AhdI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI Bpu10I
BpuEI BsaAI BsaXI BsiWI BsmBI BsrGI BssHII BssSI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EarI EcoICRI EcoRI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI ScaI
SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI