Restriction map for
pF1KE0388
(Linear) (Six-base) MAPSORT of: KIEE0388.seq Check: 8564 from: 1 to: 1830
KIEE0388.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 593 1830
Size: 515 78 1237
Fragments arranged by size:
1237 515 78
AfeI AGC'GCT
Cuts at: 0 1572 1830
Size: 1572 258
AflIII A'CryG_T
Cuts at: 0 676 1830
Size: 676 1154
AlwNI CAG_nnn'CTG
Cuts at: 0 849 1418 1782 1830
Size: 849 569 364 48
Fragments arranged by size:
849 569 364 48
ApaI G_GGCC'C
Cuts at: 0 54 69 185 1830
Size: 54 15 116 1645
Fragments arranged by size:
1645 116 54 15
ApoI r'AATT_y
Cuts at: 0 318 531 543 558 1124 1830
Size: 318 213 12 15 566 706
Fragments arranged by size:
706 566 318 213 15 12
AvaI C'yCGr_G
Cuts at: 0 45 1830
Size: 45 1785
BanII G_rGCy'C
Cuts at: 0 54 69 185 1830
Size: 54 15 116 1645
Fragments arranged by size:
1645 116 54 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1162 1830
Size: 1162 668
BglI GCCn_nnn'nGGC
Cuts at: 0 1819 1830
Size: 1819 11
Bme1580I G_kGCm'C
Cuts at: 0 54 69 185 1830
Size: 54 15 116 1645
Fragments arranged by size:
1645 116 54 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 302 1830
Size: 302 1528
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1016 1830
Size: 1016 814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1452 1830
Size: 1452 378
Bpu10I CC'TnA_GC
Cuts at: 0 202 1830
Size: 202 1628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 1830
Size: 951 879
BsaAI yAC'GTr
Cuts at: 0 1638 1830
Size: 1638 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 151 181 1830
Size: 151 30 1649
Fragments arranged by size:
1649 151 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1774 1830
Size: 1774 56
BseYI C'CCAG_C
Cuts at: 0 18 184 366 1694 1742 1830
Size: 18 166 182 1328 48 88
Fragments arranged by size:
1328 182 166 88 48 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 731 1830
Size: 310 421 1099
Fragments arranged by size:
1099 421 310
BsiEI CG_ry'CG
Cuts at: 0 28 1830
Size: 28 1802
Bsp1286I G_dGCh'C
Cuts at: 0 54 69 185 1830
Size: 54 15 116 1645
Fragments arranged by size:
1645 116 54 15
BspHI T'CATG_A
Cuts at: 0 1613 1830
Size: 1613 217
BsrDI GCAATG_nn'
Cuts at: 0 401 1830
Size: 401 1429
BsrFI r'CCGG_y
Cuts at: 0 221 1830
Size: 221 1609
BsrGI T'GTAC_A
Cuts at: 0 651 1830
Size: 651 1179
BstEII G'GTnAC_C
Cuts at: 0 1681 1830
Size: 1681 149
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1529 1830
Size: 1529 301
BstYI r'GATC_y
Cuts at: 0 1068 1752 1830
Size: 1068 684 78
Fragments arranged by size:
1068 684 78
Bsu36I CC'TnA_GG
Cuts at: 0 61 894 1830
Size: 61 833 936
Fragments arranged by size:
936 833 61
BtgI C'CryG_G
Cuts at: 0 54 148 176 1044 1229 1484 1830
Size: 54 94 28 868 185 255 346
Fragments arranged by size:
868 346 255 185 94 54 28
DraI TTT'AAA
Cuts at: 0 564 1830
Size: 564 1266
EaeI y'GGCC_r
Cuts at: 0 25 1376 1661 1830
Size: 25 1351 285 169
Fragments arranged by size:
1351 285 169 25
EagI C'GGCC_G
Cuts at: 0 25 1830
Size: 25 1805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 593 1452 1830
Size: 515 78 859 378
Fragments arranged by size:
859 515 378 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 205 1830
Size: 205 1625
EcoO109I rG'GnC_Cy
Cuts at: 0 50 65 66 181 1820 1830
Size: 50 15 1 115 1639 10
Fragments arranged by size:
1639 115 50 15 10 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 945 1830
Size: 945 885
HaeII r_GCGC'y
Cuts at: 0 131 1574 1830
Size: 131 1443 256
Fragments arranged by size:
1443 256 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 831 863 1830
Size: 151 32 648 32 967
Fragments arranged by size:
967 648 151 32 32
HincII GTy'rAC
Cuts at: 0 414 1830
Size: 414 1416
HindIII A'AGCT_T
Cuts at: 0 1115 1830
Size: 1115 715
MluI A'CGCG_T
Cuts at: 0 676 1830
Size: 676 1154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1510 1830
Size: 688 822 320
Fragments arranged by size:
822 688 320
MscI TGG'CCA
Cuts at: 0 1378 1830
Size: 1378 452
MspA1I CmG'CkG
Cuts at: 0 56 84 111 150 178 1782 1830
Size: 56 28 27 39 28 1604 48
Fragments arranged by size:
1604 56 48 39 28 28 27
NaeI GCC'GGC
Cuts at: 0 223 1830
Size: 223 1607
NcoI C'CATG_G
Cuts at: 0 1044 1229 1484 1830
Size: 1044 185 255 346
Fragments arranged by size:
1044 346 255 185
NgoMIV G'CCGG_C
Cuts at: 0 221 1830
Size: 221 1609
NspI r_CATG'y
Cuts at: 0 343 658 1830
Size: 343 315 1172
Fragments arranged by size:
1172 343 315
PasI CC'CwG_GG
Cuts at: 0 1815 1830
Size: 1815 15
PspOMI G'GGCC_C
Cuts at: 0 50 65 181 1830
Size: 50 15 116 1649
Fragments arranged by size:
1649 116 50 15
PstI C_TGCA'G
Cuts at: 0 167 1830
Size: 167 1663
PvuII CAG'CTG
Cuts at: 0 1782 1830
Size: 1782 48
SacII CC_GC'GG
Cuts at: 0 57 151 179 1830
Size: 57 94 28 1651
Fragments arranged by size:
1651 94 57 28
SexAI A'CCwGG_T
Cuts at: 0 1331 1830
Size: 1331 499
SfcI C'TryA_G
Cuts at: 0 163 1830
Size: 163 1667
SmaI CCC'GGG
Cuts at: 0 47 1830
Size: 47 1783
SmlI C'TyrA_G
Cuts at: 0 930 1830
Size: 930 900
SphI G_CATG'C
Cuts at: 0 343 1830
Size: 343 1487
SspI AAT'ATT
Cuts at: 0 719 737 1830
Size: 719 18 1093
Fragments arranged by size:
1093 719 18
StyI C'CwwG_G
Cuts at: 0 350 614 810 1044 1229 1484 1830
Size: 350 264 196 234 185 255 346
Fragments arranged by size:
350 346 264 255 234 196 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 220 847 1830
Size: 220 627 983
Fragments arranged by size:
983 627 220
TatI w'GTAC_w
Cuts at: 0 475 651 1011 1830
Size: 475 176 360 819
Fragments arranged by size:
819 475 360 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 362 394 1830
Size: 362 32 1436
Fragments arranged by size:
1436 362 32
XmaI C'CCGG_G
Cuts at: 0 45 1830
Size: 45 1785
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApaI ApoI AvaI BanII
BbsI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspHI
BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI
DraI EaeI EagI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HindIII MluI MmeI MscI MspA1I NaeI
NcoI NgoMIV NspI PasI PspOMI PstI PvuII SacII
SexAI SfcI SmaI SmlI SphI SspI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BsaI BsaBI BsaHI
BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BssHII BssSI BstAPI BstBI BstZ17I BtgZI BtsI ClaI
CspCI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HpaI KasI KpnI MfeI MslI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI