Restriction map for
pF1KE0390
(Linear) (Six-base) MAPSORT of: KIEE0390.seq Check: 6892 from: 1 to: 1464
KIEE0390.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 0 1226 1464
Size: 1226 238
AccI GT'mk_AC
Cuts at: 0 1364 1464
Size: 1364 100
Acc65I G'GTAC_C
Cuts at: 0 1325 1464
Size: 1325 139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1464
Size: 850 614
AflIII A'CryG_T
Cuts at: 0 662 1464
Size: 662 802
AhdI GACnn_n'nnGTC
Cuts at: 0 1227 1464
Size: 1227 237
AleI CACnn'nnGTG
Cuts at: 0 316 1464
Size: 316 1148
AlwNI CAG_nnn'CTG
Cuts at: 0 233 396 828 1183 1464
Size: 233 163 432 355 281
Fragments arranged by size:
432 355 281 233 163
ApaI G_GGCC'C
Cuts at: 0 486 963 1464
Size: 486 477 501
Fragments arranged by size:
501 486 477
ApaLI G'TGCA_C
Cuts at: 0 1039 1464
Size: 1039 425
ApoI r'AATT_y
Cuts at: 0 685 1464
Size: 685 779
AvaI C'yCGr_G
Cuts at: 0 91 457 651 876 949 1143 1399 1464
Size: 91 366 194 225 73 194 256 65
Fragments arranged by size:
366 256 225 194 194 91 73 65
BanI G'GyrC_C
Cuts at: 0 85 943 1325 1464
Size: 85 858 382 139
Fragments arranged by size:
858 382 139 85
BanII G_rGCy'C
Cuts at: 0 302 486 754 963 1464
Size: 302 184 268 209 501
Fragments arranged by size:
501 302 268 209 184
BbsI GAAGACnn'nnnn_
Cuts at: 0 1197 1464
Size: 1197 267
BbvCI CC'TCA_GC
Cuts at: 0 158 1004 1464
Size: 158 846 460
Fragments arranged by size:
846 460 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 857 891 1464
Size: 857 34 573
Fragments arranged by size:
857 573 34
BglI GCCn_nnn'nGGC
Cuts at: 0 461 1354 1464
Size: 461 893 110
Fragments arranged by size:
893 461 110
BglII A'GATC_T
Cuts at: 0 53 1464
Size: 53 1411
Bme1580I G_kGCm'C
Cuts at: 0 125 240 308 486 946 963 1043 1464
Size: 125 115 68 178 460 17 80 421
Fragments arranged by size:
460 421 178 125 115 80 68 17
BmgBI CAC'GTC
Cuts at: 0 663 1464
Size: 663 801
BmrI ACTGGGnnnn_n'
Cuts at: 0 112 1464
Size: 112 1352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 1464
Size: 360 1104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1042 1399 1407 1464
Size: 589 453 357 8 57
Fragments arranged by size:
589 453 357 57 8
Bpu10I CC'TnA_GC
Cuts at: 0 158 1004 1464
Size: 158 846 460
Fragments arranged by size:
846 460 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1464
Size: 1108 356
BsaI GGTCTCn'nnnn_
Cuts at: 0 516 1464
Size: 516 948
BsaAI yAC'GTr
Cuts at: 0 318 1368 1464
Size: 318 1050 96
Fragments arranged by size:
1050 318 96
BsaHI Gr'CG_yC
Cuts at: 0 1223 1238 1464
Size: 1223 15 226
Fragments arranged by size:
1223 226 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 496 526 877 907 1123 1153 1464
Size: 496 30 351 30 216 30 311
Fragments arranged by size:
496 351 311 216 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 586 1039 1404 1464
Size: 391 195 453 365 60
Fragments arranged by size:
453 391 365 195 60
BseYI C'CCAG_C
Cuts at: 0 289 442 705 747 1464
Size: 289 153 263 42 717
Fragments arranged by size:
717 289 263 153 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1231 1423 1464
Size: 847 384 192 41
Fragments arranged by size:
847 384 192 41
BsiHKAI G_wGCw'C
Cuts at: 0 877 1043 1070 1373 1464
Size: 877 166 27 303 91
Fragments arranged by size:
877 303 166 91 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 331 1294 1464
Size: 331 963 170
Fragments arranged by size:
963 331 170
Bsp1286I G_dGCh'C
Cuts at: 0 125 240 302 308 486 754 877 946
Size: 125 115 62 6 178 268 123 69
Cuts at: 946 963 1043 1070 1373 1464
Size: 17 80 27 303 91
Fragments arranged by size:
303 268 178 125 123 115 91 80
69 62 27 17 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 548 935 1464
Size: 548 387 529
Fragments arranged by size:
548 529 387
BsrBI CCG'CTC
Cuts at: 0 672 849 1464
Size: 672 177 615
Fragments arranged by size:
672 615 177
BsrFI r'CCGG_y
Cuts at: 0 60 1328 1464
Size: 60 1268 136
Fragments arranged by size:
1268 136 60
BsrGI T'GTAC_A
Cuts at: 0 893 1464
Size: 893 571
BssSI C'ACGA_G
Cuts at: 0 301 844 1069 1249 1297 1464
Size: 301 543 225 180 48 167
Fragments arranged by size:
543 301 225 180 167 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1317 1464
Size: 1317 147
BstYI r'GATC_y
Cuts at: 0 53 818 1160 1464
Size: 53 765 342 304
Fragments arranged by size:
765 342 304 53
Bsu36I CC'TnA_GG
Cuts at: 0 13 1184 1464
Size: 13 1171 280
Fragments arranged by size:
1171 280 13
BtgI C'CryG_G
Cuts at: 0 81 219 515 761 1464
Size: 81 138 296 246 703
Fragments arranged by size:
703 296 246 138 81
BtsI GCAGTG_nn'
Cuts at: 0 875 1464
Size: 875 589
DraIII CAC_nnn'GTG
Cuts at: 0 870 1464
Size: 870 594
EaeI y'GGCC_r
Cuts at: 0 58 320 673 1052 1190 1266 1464
Size: 58 262 353 379 138 76 198
Fragments arranged by size:
379 353 262 198 138 76 58
EarI CTCTTCn'nnn_
Cuts at: 0 169 239 1464
Size: 169 70 1225
Fragments arranged by size:
1225 169 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 205 1372 1464
Size: 205 1167 92
Fragments arranged by size:
1167 205 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 850 1042 1399 1407 1464
Size: 589 261 192 357 8 57
Fragments arranged by size:
589 357 261 192 57 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 244 544 811 1464
Size: 244 300 267 653
Fragments arranged by size:
653 300 267 244
EcoO109I rG'GnC_Cy
Cuts at: 0 95 425 648 834 960 1464
Size: 95 330 223 186 126 504
Fragments arranged by size:
504 330 223 186 126 95
EcoRI G'AATT_C
Cuts at: 0 685 1464
Size: 685 779
FspI TGC'GCA
Cuts at: 0 916 1464
Size: 916 548
HaeII r_GCGC'y
Cuts at: 0 296 1183 1464
Size: 296 887 281
Fragments arranged by size:
887 296 281
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1123 1155 1213 1245 1464
Size: 1123 32 58 32 219
Fragments arranged by size:
1123 219 58 32 32
KpnI G_GTAC'C
Cuts at: 0 1329 1464
Size: 1329 135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1464
Size: 358 1106
MscI TGG'CCA
Cuts at: 0 322 1268 1464
Size: 322 946 196
Fragments arranged by size:
946 322 196
MslI CAynn'nnrTG
Cuts at: 0 316 1464
Size: 316 1148
MspA1I CmG'CkG
Cuts at: 0 221 396 523 1464
Size: 221 175 127 941
Fragments arranged by size:
941 221 175 127
NaeI GCC'GGC
Cuts at: 0 62 1464
Size: 62 1402
NcoI C'CATG_G
Cuts at: 0 81 515 1464
Size: 81 434 949
Fragments arranged by size:
949 434 81
NgoMIV G'CCGG_C
Cuts at: 0 60 1464
Size: 60 1404
NspI r_CATG'y
Cuts at: 0 639 1464
Size: 639 825
PmlI CAC'GTG
Cuts at: 0 318 1464
Size: 318 1146
PpuMI rG'GwC_Cy
Cuts at: 0 95 1464
Size: 95 1369
PspOMI G'GGCC_C
Cuts at: 0 482 959 1464
Size: 482 477 505
Fragments arranged by size:
505 482 477
PstI C_TGCA'G
Cuts at: 0 545 629 932 1310 1464
Size: 545 84 303 378 154
Fragments arranged by size:
545 378 303 154 84
PvuII CAG'CTG
Cuts at: 0 396 523 1464
Size: 396 127 941
Fragments arranged by size:
941 396 127
SacII CC_GC'GG
Cuts at: 0 222 1464
Size: 222 1242
SanDI GG'GwC_CC
Cuts at: 0 95 1464
Size: 95 1369
SapI GCTCTTCn'nnn_
Cuts at: 0 169 1464
Size: 169 1295
SbfI CC_TGCA'GG
Cuts at: 0 932 1464
Size: 932 532
SfcI C'TryA_G
Cuts at: 0 541 625 928 1306 1464
Size: 541 84 303 378 158
Fragments arranged by size:
541 378 303 158 84
SmaI CCC'GGG
Cuts at: 0 93 1464
Size: 93 1371
SmlI C'TyrA_G
Cuts at: 0 1123 1464
Size: 1123 341
SphI G_CATG'C
Cuts at: 0 639 1464
Size: 639 825
StuI AGG'CCT
Cuts at: 0 740 934 1464
Size: 740 194 530
Fragments arranged by size:
740 530 194
StyI C'CwwG_G
Cuts at: 0 81 323 491 515 693 812 1464
Size: 81 242 168 24 178 119 652
Fragments arranged by size:
652 242 178 168 119 81 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 328 426 697 1464
Size: 328 98 271 767
Fragments arranged by size:
767 328 271 98
TatI w'GTAC_w
Cuts at: 0 893 1464
Size: 893 571
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 461 493 693 725 727 759 1464
Size: 461 32 200 32 2 32 705
Fragments arranged by size:
705 461 200 32 32 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 1464
Size: 315 1149
XmaI C'CCGG_G
Cuts at: 0 91 1464
Size: 91 1373
ZraI GAC'GTC
Cuts at: 0 1224 1464
Size: 1224 240
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AhdI AleI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI
BcgI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssSI BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PmlI PpuMI PspOMI PstI PvuII SacII
SanDI SapI SbfI SfcI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AlfI AloI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI
BfrBI BlpI BmtI BsaBI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrDI BssHII BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI