Restriction map for
pF1KE0402
(Linear) (Six-base) MAPSORT of: KIEE0402.seq Check: 2207 from: 1 to: 912
KIEE0402.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 752 912
Size: 752 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 857 891 912
Size: 316 541 34 21
Fragments arranged by size:
541 316 34 21
AflIII A'CryG_T
Cuts at: 0 539 912
Size: 539 373
AlwNI CAG_nnn'CTG
Cuts at: 0 223 912
Size: 223 689
ApaLI G'TGCA_C
Cuts at: 0 592 912
Size: 592 320
ApoI r'AATT_y
Cuts at: 0 459 802 912
Size: 459 343 110
Fragments arranged by size:
459 343 110
BamHI G'GATC_C
Cuts at: 0 764 912
Size: 764 148
BanII G_rGCy'C
Cuts at: 0 174 912
Size: 174 738
BbsI GAAGACnn'nnnn_
Cuts at: 0 61 912
Size: 61 851
BfrBI ATG'CAT
Cuts at: 0 343 912
Size: 343 569
Bme1580I G_kGCm'C
Cuts at: 0 596 912
Size: 596 316
BmgBI CAC'GTC
Cuts at: 0 361 912
Size: 361 551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 573 912
Size: 573 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 912
Size: 905 7
BsaAI yAC'GTr
Cuts at: 0 542 912
Size: 542 370
BseYI C'CCAG_C
Cuts at: 0 321 912
Size: 321 591
BsiHKAI G_wGCw'C
Cuts at: 0 440 596 912
Size: 440 156 316
Fragments arranged by size:
440 316 156
BsmI GAATG_Cn'
Cuts at: 0 343 850 912
Size: 343 507 62
Fragments arranged by size:
507 343 62
Bsp1286I G_dGCh'C
Cuts at: 0 174 440 596 912
Size: 174 266 156 316
Fragments arranged by size:
316 266 174 156
BstYI r'GATC_y
Cuts at: 0 764 912
Size: 764 148
BtgI C'CryG_G
Cuts at: 0 683 912
Size: 683 229
BtsI GCAGTG_nn'
Cuts at: 0 21 912
Size: 21 891
DraI TTT'AAA
Cuts at: 0 825 912
Size: 825 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 748 912
Size: 748 164
EarI CTCTTCn'nnn_
Cuts at: 0 23 155 247 650 912
Size: 23 132 92 403 262
Fragments arranged by size:
403 262 132 92 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 857 891 905 912
Size: 316 541 34 14 7
Fragments arranged by size:
541 316 34 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 179 912
Size: 179 733
EcoO109I rG'GnC_Cy
Cuts at: 0 185 354 912
Size: 185 169 558
Fragments arranged by size:
558 185 169
EcoRI G'AATT_C
Cuts at: 0 459 802 912
Size: 459 343 110
Fragments arranged by size:
459 343 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 912
Size: 258 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 109 141 912
Size: 109 32 771
Fragments arranged by size:
771 109 32
HincII GTy'rAC
Cuts at: 0 68 452 912
Size: 68 384 460
Fragments arranged by size:
460 384 68
HpaI GTT'AAC
Cuts at: 0 452 912
Size: 452 460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 912
Size: 742 170
MslI CAynn'nnrTG
Cuts at: 0 628 721 912
Size: 628 93 191
Fragments arranged by size:
628 191 93
MspA1I CmG'CkG
Cuts at: 0 325 912
Size: 325 587
NdeI CA'TA_TG
Cuts at: 0 488 912
Size: 488 424
NsiI A_TGCA'T
Cuts at: 0 345 912
Size: 345 567
PmlI CAC'GTG
Cuts at: 0 542 912
Size: 542 370
PpuMI rG'GwC_Cy
Cuts at: 0 354 912
Size: 354 558
PstI C_TGCA'G
Cuts at: 0 201 912
Size: 201 711
PvuII CAG'CTG
Cuts at: 0 325 912
Size: 325 587
SapI GCTCTTCn'nnn_
Cuts at: 0 155 650 912
Size: 155 495 262
Fragments arranged by size:
495 262 155
SfcI C'TryA_G
Cuts at: 0 197 912
Size: 197 715
StyI C'CwwG_G
Cuts at: 0 384 912
Size: 384 528
TatI w'GTAC_w
Cuts at: 0 248 713 912
Size: 248 465 199
Fragments arranged by size:
465 248 199
XbaI T'CTAG_A
Cuts at: 0 738 912
Size: 738 174
XmnI GAAnn'nnTTC
Cuts at: 0 301 850 912
Size: 301 549 62
Fragments arranged by size:
549 301 62
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI ApoI BamHI BanII
BbsI BfrBI Bme1580I BmgBI BplI BpmI BsaAI BseYI
BsiHKAI BsmI Bsp1286I BstYI BtgI BtsI DraI DrdI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII
HpaI MmeI MslI MspA1I NdeI NsiI PmlI PpuMI
PstI PvuII SapI SfcI StyI TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmrI BmtI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII EaeI EagI EciI EcoICRI
EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XcmI XhoI XmaI ZraI