(Linear) (Six-base) MAPSORT of: KIEE0403.seq  Check: 3632  from: 1  to: 639



KIEE0403.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     639 
   Size:        241     398
 
AleI CACnn'nnGTG

Cuts at:      0     610     639 
   Size:        610      29
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     433     639 
   Size:        433     206
 
AlwNI CAG_nnn'CTG

Cuts at:      0     157     639 
   Size:        157     482
 
ApoI r'AATT_y

Cuts at:      0     163     639 
   Size:        163     476
 
BamHI G'GATC_C

Cuts at:      0      93     639 
   Size:         93     546
 
BanI G'GyrC_C

Cuts at:      0     409     639 
   Size:        409     230
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     397     541     639 
   Size:        397     144      98

  Fragments arranged by size: 

                 397     144      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     639 
   Size:        619      20
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     262     292     639 
   Size:        262      30     347

  Fragments arranged by size: 

                 347     262      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     280     639 
   Size:        280     359
 
BspHI T'CATG_A

Cuts at:      0     314     639 
   Size:        314     325
 
BsrFI r'CCGG_y

Cuts at:      0     506     639 
   Size:        506     133
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     235     639 
   Size:        235     404
 
BstYI r'GATC_y

Cuts at:      0      93     639 
   Size:         93     546
 
Bsu36I CC'TnA_GG

Cuts at:      0     512     639 
   Size:        512     127
 
BtgI C'CryG_G

Cuts at:      0      63     470     639 
   Size:         63     407     169

  Fragments arranged by size: 

                 407     169      63
 
BtsI GCAGTG_nn'

Cuts at:      0      87     639 
   Size:         87     552
 
DraIII CAC_nnn'GTG

Cuts at:      0     308     639 
   Size:        308     331
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     639 
   Size:        241     398
 
EcoO109I rG'GnC_Cy

Cuts at:      0      55     285     639 
   Size:         55     230     354

  Fragments arranged by size: 

                 354     230      55
 
EcoRV GAT'ATC

Cuts at:      0     326     639 
   Size:        326     313
 
MslI CAynn'nnrTG

Cuts at:      0     610     639 
   Size:        610      29
 
MspA1I CmG'CkG

Cuts at:      0     537     639 
   Size:        537     102
 
NcoI C'CATG_G

Cuts at:      0      63     470     639 
   Size:         63     407     169

  Fragments arranged by size: 

                 407     169      63
 
PasI CC'CwG_GG

Cuts at:      0     282     639 
   Size:        282     357
 
PfoI T'CCnGG_A

Cuts at:      0     587     639 
   Size:        587      52
 
PstI C_TGCA'G

Cuts at:      0      82     639 
   Size:         82     557
 
SfcI C'TryA_G

Cuts at:      0      78     639 
   Size:         78     561
 
SmlI C'TyrA_G

Cuts at:      0     634     639 
   Size:        634       5
 
StyI C'CwwG_G

Cuts at:      0      63     470     500     639 
   Size:         63     407      30     139

  Fragments arranged by size: 

                 407     139      63      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      96     138     639 
   Size:         96      42     501

  Fragments arranged by size: 

                 501      96      42
 
TatI w'GTAC_w

Cuts at:      0     275     639 
   Size:        275     364
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      29      61     450     482     639 
   Size:         29      32     389      32     157

  Fragments arranged by size: 

                 389     157      32      32      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     420     541     639 
   Size:        420     121      98

  Fragments arranged by size: 

                 420     121      98
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AlwNI     ApoI    BamHI     BanI    BciVI
    BpuEI    BsaXI    BseRI    BspHI    BsrFI   BstAPI    BstYI   Bsu36I
     BtgI     BtsI   DraIII  Eco57MI EcoO109I    EcoRV     MslI   MspA1I
     NcoI     PasI     PfoI     PstI     SfcI     SmlI     StyI    TaqII
     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI