(Linear) (Six-base) MAPSORT of: KIEE0407.seq  Check: 6590  from: 1  to: 762



KIEE0407.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

ApoI r'AATT_y

Cuts at:      0     397     580     762 
   Size:        397     183     182

  Fragments arranged by size: 

                 397     183     182
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     233     266     762 
   Size:        233      33     496

  Fragments arranged by size: 

                 496     233      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     279     588     762 
   Size:        279     309     174

  Fragments arranged by size: 

                 309     279     174
 
BfrBI ATG'CAT

Cuts at:      0     193     468     762 
   Size:        193     275     294

  Fragments arranged by size: 

                 294     275     193
 
BglI GCCn_nnn'nGGC

Cuts at:      0      72     762 
   Size:         72     690
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     706     762 
   Size:        296     410      56

  Fragments arranged by size: 

                 410     296      56
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     616     762 
   Size:        616     146
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      59     762 
   Size:         59     703
 
BsrFI r'CCGG_y

Cuts at:      0      84     762 
   Size:         84     678
 
BtgI C'CryG_G

Cuts at:      0      69     762 
   Size:         69     693
 
EaeI y'GGCC_r

Cuts at:      0     139     762 
   Size:        139     623
 
EcoO109I rG'GnC_Cy

Cuts at:      0      65     762 
   Size:         65     697
 
EcoRV GAT'ATC

Cuts at:      0     507     762 
   Size:        507     255
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     294     326     762 
   Size:        294      32     436

  Fragments arranged by size: 

                 436     294      32
 
HincII GTy'rAC

Cuts at:      0     260     762 
   Size:        260     502
 
HpaI GTT'AAC

Cuts at:      0     260     762 
   Size:        260     502
 
MfeI C'AATT_G

Cuts at:      0     245     762 
   Size:        245     517
 
MslI CAynn'nnrTG

Cuts at:      0     356     762 
   Size:        356     406
 
MspA1I CmG'CkG

Cuts at:      0      71     762 
   Size:         71     691
 
NaeI GCC'GGC

Cuts at:      0      86     762 
   Size:         86     676
 
NgoMIV G'CCGG_C

Cuts at:      0      84     762 
   Size:         84     678
 
NsiI A_TGCA'T

Cuts at:      0     195     470     762 
   Size:        195     275     292

  Fragments arranged by size: 

                 292     275     195
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     195     762 
   Size:        195     567
 
SacII CC_GC'GG

Cuts at:      0      72     762 
   Size:         72     690
 
SfcI C'TryA_G

Cuts at:      0     575     762 
   Size:        575     187
 
SmlI C'TyrA_G

Cuts at:      0     311     685     762 
   Size:        311     374      77

  Fragments arranged by size: 

                 374     311      77
 
StyI C'CwwG_G

Cuts at:      0      36     119     762 
   Size:         36      83     643

  Fragments arranged by size: 

                 643      83      36
 
TatI w'GTAC_w

Cuts at:      0     333     762 
   Size:        333     429
 
Tth111I GACn'n_nGTC

Cuts at:      0     376     762 
   Size:        376     386
 

 Enzymes that do cut:

     ApoI     BaeI    BciVI    BfrBI     BglI    BpuEI     BsaI    BseRI
    BsrFI     BtgI     EaeI EcoO109I    EcoRV    Hin4I   HincII     HpaI
     MfeI     MslI   MspA1I     NaeI   NgoMIV     NsiI    PflMI    SacII
     SfcI     SmlI     StyI     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI    HaeII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI