Restriction map for
pF1KE0408
(Linear) (Six-base) MAPSORT of: KIEE0408.seq Check: 4269 from: 1 to: 987
KIEE0408.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 210 987
Size: 210 777
AccI GT'mk_AC
Cuts at: 0 647 987
Size: 647 340
Acc65I G'GTAC_C
Cuts at: 0 823 987
Size: 823 164
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 113 179 987
Size: 50 63 66 808
Fragments arranged by size:
808 66 63 50
AlwNI CAG_nnn'CTG
Cuts at: 0 238 987
Size: 238 749
ApoI r'AATT_y
Cuts at: 0 248 987
Size: 248 739
BanI G'GyrC_C
Cuts at: 0 222 823 987
Size: 222 601 164
Fragments arranged by size:
601 222 164
BanII G_rGCy'C
Cuts at: 0 17 497 692 987
Size: 17 480 195 295
Fragments arranged by size:
480 295 195 17
BbvCI CC'TCA_GC
Cuts at: 0 101 987
Size: 101 886
BciVI GTATCCnnnnn_n'
Cuts at: 0 807 987
Size: 807 180
Bme1580I G_kGCm'C
Cuts at: 0 123 227 715 987
Size: 123 104 488 272
Fragments arranged by size:
488 272 123 104
BmrI ACTGGGnnnn_n'
Cuts at: 0 666 987
Size: 666 321
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 72 543 987
Size: 72 471 444
Fragments arranged by size:
471 444 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 538 886 967 987
Size: 349 189 348 81 20
Fragments arranged by size:
349 348 189 81 20
Bpu10I CC'TnA_GC
Cuts at: 0 101 987
Size: 101 886
BsaWI w'CCGG_w
Cuts at: 0 588 987
Size: 588 399
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 52 55 64 772 781 784 964
Size: 49 3 3 9 708 9 3 180
Cuts at: 964 967 987
Size: 3 20
Fragments arranged by size:
708 180 49 20 9 9 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 16 853 987
Size: 16 837 134
Fragments arranged by size:
837 134 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 987
Size: 625 362
BsiHKAI G_wGCw'C
Cuts at: 0 56 497 987
Size: 56 441 490
Fragments arranged by size:
490 441 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 514 987
Size: 514 473
Bsp1286I G_dGCh'C
Cuts at: 0 17 56 123 227 497 692 715 987
Size: 17 39 67 104 270 195 23 272
Fragments arranged by size:
272 270 195 104 67 39 23 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 987
Size: 210 777
BsrFI r'CCGG_y
Cuts at: 0 202 987
Size: 202 785
BssSI C'ACGA_G
Cuts at: 0 424 987
Size: 424 563
Bsu36I CC'TnA_GG
Cuts at: 0 239 987
Size: 239 748
BtsI GCAGTG_nn'
Cuts at: 0 316 987
Size: 316 671
EarI CTCTTCn'nnn_
Cuts at: 0 26 41 987
Size: 26 15 946
Fragments arranged by size:
946 26 15
EcoICRI GAG'CTC
Cuts at: 0 495 987
Size: 495 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 113 179 349 538 886 967 987
Size: 50 63 66 170 189 348 81 20
Fragments arranged by size:
348 189 170 81 66 63 50 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 304 946 987
Size: 304 642 41
Fragments arranged by size:
642 304 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 987
Size: 298 32 657
Fragments arranged by size:
657 298 32
HincII GTy'rAC
Cuts at: 0 267 987
Size: 267 720
KpnI G_GTAC'C
Cuts at: 0 827 987
Size: 827 160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 760 987
Size: 506 254 227
Fragments arranged by size:
506 254 227
MspA1I CmG'CkG
Cuts at: 0 912 987
Size: 912 75
NaeI GCC'GGC
Cuts at: 0 204 987
Size: 204 783
NdeI CA'TA_TG
Cuts at: 0 624 987
Size: 624 363
NgoMIV G'CCGG_C
Cuts at: 0 202 987
Size: 202 785
PstI C_TGCA'G
Cuts at: 0 911 987
Size: 911 76
SacI G_AGCT'C
Cuts at: 0 497 987
Size: 497 490
SfcI C'TryA_G
Cuts at: 0 498 648 907 936 987
Size: 498 150 259 29 51
Fragments arranged by size:
498 259 150 51 29
StyI C'CwwG_G
Cuts at: 0 259 818 987
Size: 259 559 169
Fragments arranged by size:
559 259 169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 543 987
Size: 543 444
XmnI GAAnn'nnTTC
Cuts at: 0 703 987
Size: 703 284
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApoI BanI BanII
BbvCI BciVI Bme1580I BmrI BplI BpmI Bpu10I BsaWI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI
BssSI Bsu36I BtsI EarI EcoICRI Eco57MI EcoNI Hin4I
HincII KpnI MmeI MspA1I NaeI NdeI NgoMIV PstI
SacI SfcI StyI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MluI MscI MslI NarI NcoI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI ZraI