(Linear) (Six-base) MAPSORT of: KIEE0411.seq  Check: 1073  from: 1  to: 933



KIEE0411.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     449     933 
   Size:        449     484
 
Acc65I G'GTAC_C

Cuts at:      0     849     933 
   Size:        849      84
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     747     933 
   Size:        598     149     186

  Fragments arranged by size: 

                 598     186     149
 
AflIII A'CryG_T

Cuts at:      0     695     933 
   Size:        695     238
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     234     266     933 
   Size:        234      32     667

  Fragments arranged by size: 

                 667     234      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     770     933 
   Size:        770     163
 
ApoI r'AATT_y

Cuts at:      0     886     933 
   Size:        886      47
 
AseI AT'TA_AT

Cuts at:      0     499     933 
   Size:        499     434
 
AvaI C'yCGr_G

Cuts at:      0     237     933 
   Size:        237     696
 
BanI G'GyrC_C

Cuts at:      0     849     933 
   Size:        849      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      52     933 
   Size:         52     881
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     899     933 
   Size:        899      34
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     841     933 
   Size:        841      92
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     138     168     933 
   Size:        138      30     765

  Fragments arranged by size: 

                 765     138      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     933 
   Size:        566     367
 
BsmI GAATG_Cn'

Cuts at:      0     126     295     484     933 
   Size:        126     169     189     449

  Fragments arranged by size: 

                 449     189     169     126
 
BspHI T'CATG_A

Cuts at:      0     438     933 
   Size:        438     495
 
BsrDI GCAATG_nn'

Cuts at:      0     200     933 
   Size:        200     733
 
BsrGI T'GTAC_A

Cuts at:      0     692     933 
   Size:        692     241
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     831     933 
   Size:        831     102
 
BtsI GCAGTG_nn'

Cuts at:      0     275     878     933 
   Size:        275     603      55

  Fragments arranged by size: 

                 603     275      55
 
ClaI AT'CG_AT

Cuts at:      0     561     933 
   Size:        561     372
 
DraI TTT'AAA

Cuts at:      0      60     567     933 
   Size:         60     507     366

  Fragments arranged by size: 

                 507     366      60
 
EarI CTCTTCn'nnn_

Cuts at:      0     524     766     933 
   Size:        524     242     167

  Fragments arranged by size: 

                 524     242     167
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     747     933 
   Size:        598     149     186

  Fragments arranged by size: 

                 598     186     149
 
KpnI G_GTAC'C

Cuts at:      0     853     933 
   Size:        853      80
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     291     537     933 
   Size:        291     246     396

  Fragments arranged by size: 

                 396     291     246
 
MslI CAynn'nnrTG

Cuts at:      0     785     933 
   Size:        785     148
 
NspI r_CATG'y

Cuts at:      0      26     542     699     933 
   Size:         26     516     157     234

  Fragments arranged by size: 

                 516     234     157      26
 
PciI A'CATG_T

Cuts at:      0     695     933 
   Size:        695     238
 
SmlI C'TyrA_G

Cuts at:      0     820     933 
   Size:        820     113
 
SphI G_CATG'C

Cuts at:      0      26     933 
   Size:         26     907
 
SspI AAT'ATT

Cuts at:      0     133     933 
   Size:        133     800
 
StuI AGG'CCT

Cuts at:      0     632     933 
   Size:        632     301
 
StyI C'CwwG_G

Cuts at:      0      27     933 
   Size:         27     906
 
TatI w'GTAC_w

Cuts at:      0     426     433     692     933 
   Size:        426       7     259     241

  Fragments arranged by size: 

                 426     259     241       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     138     170     933 
   Size:        138      32     763

  Fragments arranged by size: 

                 763     138      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     807     933 
   Size:        807     126
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AloI    AlwNI     ApoI     AseI
     AvaI     BanI     BbsI    BciVI    BpuEI    BsaXI     BsgI     BsmI
    BspHI    BsrDI    BsrGI   BstAPI     BtsI     ClaI     DraI     EarI
  Eco57MI     KpnI     MmeI     MslI     NspI     PciI     SmlI     SphI
     SspI     StuI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI