Restriction map for
pF1KE0412
(Linear) (Six-base) MAPSORT of: KIEE0412.seq Check: 7465 from: 1 to: 729
KIEE0412.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
Acc65I G'GTAC_C
Cuts at: 0 659 729
Size: 659 70
AflIII A'CryG_T
Cuts at: 0 343 729
Size: 343 386
ApaI G_GGCC'C
Cuts at: 0 46 71 105 729
Size: 46 25 34 624
Fragments arranged by size:
624 46 34 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 643 676 729
Size: 643 33 53
Fragments arranged by size:
643 53 33
BanI G'GyrC_C
Cuts at: 0 659 729
Size: 659 70
BanII G_rGCy'C
Cuts at: 0 46 71 105 131 729
Size: 46 25 34 26 598
Fragments arranged by size:
598 46 34 26 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 660 729
Size: 660 69
BlpI GC'TnA_GC
Cuts at: 0 514 729
Size: 514 215
Bme1580I G_kGCm'C
Cuts at: 0 46 71 97 105 390 729
Size: 46 25 26 8 285 339
Fragments arranged by size:
339 285 46 26 25 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 460 729
Size: 460 269
BsaHI Gr'CG_yC
Cuts at: 0 59 729
Size: 59 670
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 139 225 492 729
Size: 136 3 86 267 237
Fragments arranged by size:
267 237 136 86 3
BseYI C'CCAG_C
Cuts at: 0 64 268 716 729
Size: 64 204 448 13
Fragments arranged by size:
448 204 64 13
BsiEI CG_ry'CG
Cuts at: 0 53 729
Size: 53 676
Bsp1286I G_dGCh'C
Cuts at: 0 46 71 97 105 131 390 729
Size: 46 25 26 8 26 259 339
Fragments arranged by size:
339 259 46 26 26 25 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 145 729
Size: 145 584
BsrBI CCG'CTC
Cuts at: 0 513 729
Size: 513 216
BsrFI r'CCGG_y
Cuts at: 0 48 149 729
Size: 48 101 580
Fragments arranged by size:
580 101 48
BtgI C'CryG_G
Cuts at: 0 53 203 416 729
Size: 53 150 213 313
Fragments arranged by size:
313 213 150 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 177 729
Size: 177 552
EaeI y'GGCC_r
Cuts at: 0 24 50 413 729
Size: 24 26 363 316
Fragments arranged by size:
363 316 26 24
EagI C'GGCC_G
Cuts at: 0 50 729
Size: 50 679
EcoO109I rG'GnC_Cy
Cuts at: 0 42 101 102 521 712 729
Size: 42 59 1 419 191 17
Fragments arranged by size:
419 191 59 42 17 1
HincII GTy'rAC
Cuts at: 0 333 729
Size: 333 396
KpnI G_GTAC'C
Cuts at: 0 663 729
Size: 663 66
MslI CAynn'nnrTG
Cuts at: 0 196 592 729
Size: 196 396 137
Fragments arranged by size:
396 196 137
MspA1I CmG'CkG
Cuts at: 0 55 64 159 180 729
Size: 55 9 95 21 549
Fragments arranged by size:
549 95 55 21 9
NaeI GCC'GGC
Cuts at: 0 50 729
Size: 50 679
NgoMIV G'CCGG_C
Cuts at: 0 48 729
Size: 48 681
NspI r_CATG'y
Cuts at: 0 347 729
Size: 347 382
PasI CC'CwG_GG
Cuts at: 0 186 390 729
Size: 186 204 339
Fragments arranged by size:
339 204 186
PciI A'CATG_T
Cuts at: 0 343 729
Size: 343 386
PpuMI rG'GwC_Cy
Cuts at: 0 521 729
Size: 521 208
PspOMI G'GGCC_C
Cuts at: 0 42 67 101 729
Size: 42 25 34 628
Fragments arranged by size:
628 42 34 25
PstI C_TGCA'G
Cuts at: 0 179 729
Size: 179 550
PvuII CAG'CTG
Cuts at: 0 180 729
Size: 180 549
SacII CC_GC'GG
Cuts at: 0 56 729
Size: 56 673
SexAI A'CCwGG_T
Cuts at: 0 662 729
Size: 662 67
SfcI C'TryA_G
Cuts at: 0 175 729
Size: 175 554
StyI C'CwwG_G
Cuts at: 0 107 729
Size: 107 622
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 729
Size: 667 62
Enzymes that do cut:
Acc65I AflIII ApaI BaeI BanI BanII BbsI BlpI
Bme1580I BsaI BsaHI BseRI BseYI BsiEI Bsp1286I BspMI
BsrBI BsrFI BtgI BtgZI EaeI EagI EcoO109I HincII
KpnI MslI MspA1I NaeI NgoMIV NspI PasI PciI
PpuMI PspOMI PstI PvuII SacII SexAI SfcI StyI
TaqII
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI