Restriction map for
pF1KE0416
(Linear) (Six-base) MAPSORT of: KIEE0416.seq Check: 9748 from: 1 to: 1056
KIEE0416.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 445 586 913 1056
Size: 271 174 141 327 143
Fragments arranged by size:
327 271 174 143 141
AflIII A'CryG_T
Cuts at: 0 751 1056
Size: 751 305
AleI CACnn'nnGTG
Cuts at: 0 55 1033 1056
Size: 55 978 23
Fragments arranged by size:
978 55 23
AlwNI CAG_nnn'CTG
Cuts at: 0 279 716 789 858 1056
Size: 279 437 73 69 198
Fragments arranged by size:
437 279 198 73 69
AvaI C'yCGr_G
Cuts at: 0 98 1031 1056
Size: 98 933 25
Fragments arranged by size:
933 98 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1030 1056
Size: 1030 26
BanI G'GyrC_C
Cuts at: 0 46 384 1056
Size: 46 338 672
Fragments arranged by size:
672 338 46
BanII G_rGCy'C
Cuts at: 0 116 998 1056
Size: 116 882 58
Fragments arranged by size:
882 116 58
BlpI GC'TnA_GC
Cuts at: 0 859 1056
Size: 859 197
Bme1580I G_kGCm'C
Cuts at: 0 49 374 389 956 1009 1056
Size: 49 325 15 567 53 47
Fragments arranged by size:
567 325 53 49 47 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 964 1056
Size: 158 806 92
Fragments arranged by size:
806 158 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 799 1056
Size: 100 699 257
Fragments arranged by size:
699 257 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 108 544 1056
Size: 108 436 512
Fragments arranged by size:
512 436 108
BsaWI w'CCGG_w
Cuts at: 0 776 1056
Size: 776 280
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 844 1056
Size: 571 273 212
Fragments arranged by size:
571 273 212
BseYI C'CCAG_C
Cuts at: 0 851 1056
Size: 851 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 1056
Size: 951 105
BsiHKAI G_wGCw'C
Cuts at: 0 116 296 1056
Size: 116 180 760
Fragments arranged by size:
760 180 116
Bsp1286I G_dGCh'C
Cuts at: 0 49 116 296 374 389 956 998 1009
Size: 49 67 180 78 15 567 42 11
Cuts at: 1009 1056
Size: 47
Fragments arranged by size:
567 180 78 67 49 47 42 15
11
BspEI T'CCGG_A
Cuts at: 0 776 1056
Size: 776 280
BsrBI CCG'CTC
Cuts at: 0 8 531 1056
Size: 8 523 525
Fragments arranged by size:
525 523 8
BsrFI r'CCGG_y
Cuts at: 0 86 1056
Size: 86 970
BsrGI T'GTAC_A
Cuts at: 0 1046 1056
Size: 1046 10
BssSI C'ACGA_G
Cuts at: 0 391 1056
Size: 391 665
BstAPI GCAn_nnn'nTGC
Cuts at: 0 789 974 1056
Size: 789 185 82
Fragments arranged by size:
789 185 82
BstEII G'GTnAC_C
Cuts at: 0 60 1056
Size: 60 996
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 397 1056
Size: 57 340 659
Fragments arranged by size:
659 340 57
BstYI r'GATC_y
Cuts at: 0 444 1056
Size: 444 612
Bsu36I CC'TnA_GG
Cuts at: 0 875 1056
Size: 875 181
DraI TTT'AAA
Cuts at: 0 152 1056
Size: 152 904
DraIII CAC_nnn'GTG
Cuts at: 0 974 1056
Size: 974 82
EaeI y'GGCC_r
Cuts at: 0 510 1056
Size: 510 546
EarI CTCTTCn'nnn_
Cuts at: 0 769 889 1056
Size: 769 120 167
Fragments arranged by size:
769 167 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 613 1056
Size: 613 443
EcoICRI GAG'CTC
Cuts at: 0 114 1056
Size: 114 942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 271 445 586 913 964 1056
Size: 158 113 174 141 327 51 92
Fragments arranged by size:
327 174 158 141 113 92 51
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 250 1056
Size: 250 806
HaeII r_GCGC'y
Cuts at: 0 733 807 902 1056
Size: 733 74 95 154
Fragments arranged by size:
733 154 95 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 601 633 1010 1042 1056
Size: 336 32 233 32 377 32 14
Fragments arranged by size:
377 336 233 32 32 32 14
MslI CAynn'nnrTG
Cuts at: 0 55 140 395 1033 1056
Size: 55 85 255 638 23
Fragments arranged by size:
638 255 85 55 23
MspA1I CmG'CkG
Cuts at: 0 276 524 795 855 1056
Size: 276 248 271 60 201
Fragments arranged by size:
276 271 248 201 60
NspI r_CATG'y
Cuts at: 0 651 755 1056
Size: 651 104 301
Fragments arranged by size:
651 301 104
PciI A'CATG_T
Cuts at: 0 751 1056
Size: 751 305
PflMI CCAn_nnn'nTGG
Cuts at: 0 249 1056
Size: 249 807
PfoI T'CCnGG_A
Cuts at: 0 174 605 836 1056
Size: 174 431 231 220
Fragments arranged by size:
431 231 220 174
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 457 489 1056
Size: 457 32 567
Fragments arranged by size:
567 457 32
PstI C_TGCA'G
Cuts at: 0 632 692 794 1018 1056
Size: 632 60 102 224 38
Fragments arranged by size:
632 224 102 60 38
PvuII CAG'CTG
Cuts at: 0 855 1056
Size: 855 201
SacI G_AGCT'C
Cuts at: 0 116 1056
Size: 116 940
SapI GCTCTTCn'nnn_
Cuts at: 0 889 1056
Size: 889 167
ScaI AGT'ACT
Cuts at: 0 655 1056
Size: 655 401
SexAI A'CCwGG_T
Cuts at: 0 380 1056
Size: 380 676
SfcI C'TryA_G
Cuts at: 0 628 688 790 1014 1056
Size: 628 60 102 224 42
Fragments arranged by size:
628 224 102 60 42
SmaI CCC'GGG
Cuts at: 0 100 1033 1056
Size: 100 933 23
Fragments arranged by size:
933 100 23
SmlI C'TyrA_G
Cuts at: 0 115 814 1056
Size: 115 699 242
Fragments arranged by size:
699 242 115
SphI G_CATG'C
Cuts at: 0 651 1056
Size: 651 405
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 334 568 1056
Size: 130 204 234 488
Fragments arranged by size:
488 234 204 130
TatI w'GTAC_w
Cuts at: 0 74 653 1046 1056
Size: 74 579 393 10
Fragments arranged by size:
579 393 74 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 170 202 946 978 1056
Size: 170 32 744 32 78
Fragments arranged by size:
744 170 78 32 32
XbaI T'CTAG_A
Cuts at: 0 453 1056
Size: 453 603
XmaI C'CCGG_G
Cuts at: 0 98 1031 1056
Size: 98 933 25
Fragments arranged by size:
933 98 25
Enzymes that do cut:
AcuI AflIII AleI AlwNI AvaI BaeI BanI BanII
BlpI Bme1580I BpmI BpuEI BsaI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BsrBI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI Bsu36I DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI FalI HaeII Hin4I MslI
MspA1I NspI PciI PflMI PfoI PsrI PstI PvuII
SacI SapI ScaI SexAI SfcI SmaI SmlI SphI
TaqII TatI TstI XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI
BsmBI BspHI BspMI BsrDI BssHII BstBI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DrdI EagI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
StyI SwaI Tth111I XcmI XhoI XmnI ZraI