Restriction map for
pF1KE0418
(Linear) (Six-base) MAPSORT of: KIEE0418.seq Check: 1256 from: 1 to: 1269
KIEE0418.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 727 897 921 1269
Size: 587 140 170 24 348
Fragments arranged by size:
587 348 170 140 24
AlwNI CAG_nnn'CTG
Cuts at: 0 1148 1269
Size: 1148 121
ApaI G_GGCC'C
Cuts at: 0 66 749 1269
Size: 66 683 520
Fragments arranged by size:
683 520 66
ApoI r'AATT_y
Cuts at: 0 304 1269
Size: 304 965
AvaI C'yCGr_G
Cuts at: 0 298 495 1269
Size: 298 197 774
Fragments arranged by size:
774 298 197
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 961 994 1269
Size: 961 33 275
Fragments arranged by size:
961 275 33
BamHI G'GATC_C
Cuts at: 0 221 1269
Size: 221 1048
BanII G_rGCy'C
Cuts at: 0 66 749 805 1269
Size: 66 683 56 464
Fragments arranged by size:
683 464 66 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 575 688 1269
Size: 575 113 581
Fragments arranged by size:
581 575 113
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 33 100 134 1269
Size: 33 67 34 1135
Fragments arranged by size:
1135 67 34 33
BglI GCCn_nnn'nGGC
Cuts at: 0 667 1269
Size: 667 602
Bme1580I G_kGCm'C
Cuts at: 0 66 299 749 1269
Size: 66 233 450 520
Fragments arranged by size:
520 450 233 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 824 1149 1269
Size: 824 325 120
Fragments arranged by size:
824 325 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 987 1201 1269
Size: 871 116 214 68
Fragments arranged by size:
871 214 116 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 814 1269
Size: 493 321 455
Fragments arranged by size:
493 455 321
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 318 1269
Size: 268 50 951
Fragments arranged by size:
951 268 50
BsaAI yAC'GTr
Cuts at: 0 294 1269
Size: 294 975
BsaHI Gr'CG_yC
Cuts at: 0 197 825 1269
Size: 197 628 444
Fragments arranged by size:
628 444 197
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 122 152 1269
Size: 122 30 1117
Fragments arranged by size:
1117 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 797 1269
Size: 475 322 472
Fragments arranged by size:
475 472 322
BseYI C'CCAG_C
Cuts at: 0 442 533 1269
Size: 442 91 736
Fragments arranged by size:
736 442 91
BsiEI CG_ry'CG
Cuts at: 0 30 1269
Size: 30 1239
BsmI GAATG_Cn'
Cuts at: 0 1095 1269
Size: 1095 174
BsmBI CGTCTCn'nnnn_
Cuts at: 0 832 1269
Size: 832 437
Bsp1286I G_dGCh'C
Cuts at: 0 66 299 749 805 1269
Size: 66 233 450 56 464
Fragments arranged by size:
464 450 233 66 56
BsrBI CCG'CTC
Cuts at: 0 133 1269
Size: 133 1136
BsrFI r'CCGG_y
Cuts at: 0 1014 1269
Size: 1014 255
BsrGI T'GTAC_A
Cuts at: 0 1073 1269
Size: 1073 196
BssSI C'ACGA_G
Cuts at: 0 397 663 1269
Size: 397 266 606
Fragments arranged by size:
606 397 266
BstEII G'GTnAC_C
Cuts at: 0 138 1269
Size: 138 1131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 1269
Size: 159 1110
BstYI r'GATC_y
Cuts at: 0 221 986 1269
Size: 221 765 283
Fragments arranged by size:
765 283 221
BtgI C'CryG_G
Cuts at: 0 125 575 878 1221 1269
Size: 125 450 303 343 48
Fragments arranged by size:
450 343 303 125 48
BtsI GCAGTG_nn'
Cuts at: 0 17 326 1269
Size: 17 309 943
Fragments arranged by size:
943 309 17
DraIII CAC_nnn'GTG
Cuts at: 0 873 1269
Size: 873 396
DrdI GACnn_nn'nnGTC
Cuts at: 0 412 577 1269
Size: 412 165 692
Fragments arranged by size:
692 412 165
EaeI y'GGCC_r
Cuts at: 0 27 77 149 161 288 359 881 1269
Size: 27 50 72 12 127 71 522 388
Fragments arranged by size:
522 388 127 72 71 50 27 12
EagI C'GGCC_G
Cuts at: 0 27 1269
Size: 27 1242
EarI CTCTTCn'nnn_
Cuts at: 0 638 1269
Size: 638 631
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 649 687 1269
Size: 649 38 582
Fragments arranged by size:
649 582 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 727 871 897 921 987 1201 1269
Size: 587 140 144 26 24 66 214 68
Fragments arranged by size:
587 214 144 140 68 66 26 24
EcoO109I rG'GnC_Cy
Cuts at: 0 63 405 551 605 745 918 1269
Size: 63 342 146 54 140 173 351
Fragments arranged by size:
351 342 173 146 140 63 54
EcoRI G'AATT_C
Cuts at: 0 304 1269
Size: 304 965
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 534 1269
Size: 534 735
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 1269
Size: 1174 32 63
Fragments arranged by size:
1174 63 32
MscI TGG'CCA
Cuts at: 0 163 361 883 1269
Size: 163 198 522 386
Fragments arranged by size:
522 386 198 163
MslI CAynn'nnrTG
Cuts at: 0 736 1054 1075 1269
Size: 736 318 21 194
Fragments arranged by size:
736 318 194 21
MspA1I CmG'CkG
Cuts at: 0 193 234 334 533 849 1269
Size: 193 41 100 199 316 420
Fragments arranged by size:
420 316 199 193 100 41
NaeI GCC'GGC
Cuts at: 0 1016 1269
Size: 1016 253
NgoMIV G'CCGG_C
Cuts at: 0 1014 1269
Size: 1014 255
PasI CC'CwG_GG
Cuts at: 0 59 922 1269
Size: 59 863 347
Fragments arranged by size:
863 347 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 741 1135 1269
Size: 357 384 394 134
Fragments arranged by size:
394 384 357 134
PmlI CAC'GTG
Cuts at: 0 294 1269
Size: 294 975
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 1269
Size: 86 32 1151
Fragments arranged by size:
1151 86 32
PpuMI rG'GwC_Cy
Cuts at: 0 405 551 605 918 1269
Size: 405 146 54 313 351
Fragments arranged by size:
405 351 313 146 54
PsiI TTA'TAA
Cuts at: 0 587 1269
Size: 587 682
PspOMI G'GGCC_C
Cuts at: 0 62 745 1269
Size: 62 683 524
Fragments arranged by size:
683 524 62
PstI C_TGCA'G
Cuts at: 0 716 1269
Size: 716 553
PvuII CAG'CTG
Cuts at: 0 533 1269
Size: 533 736
SanDI GG'GwC_CC
Cuts at: 0 551 918 1269
Size: 551 367 351
Fragments arranged by size:
551 367 351
SfcI C'TryA_G
Cuts at: 0 528 712 1269
Size: 528 184 557
Fragments arranged by size:
557 528 184
SmaI CCC'GGG
Cuts at: 0 300 1269
Size: 300 969
SmlI C'TyrA_G
Cuts at: 0 508 829 1269
Size: 508 321 440
Fragments arranged by size:
508 440 321
StuI AGG'CCT
Cuts at: 0 86 1269
Size: 86 1183
StyI C'CwwG_G
Cuts at: 0 164 1269
Size: 164 1105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 433 1269
Size: 433 836
TatI w'GTAC_w
Cuts at: 0 1073 1269
Size: 1073 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 847 859 879 891 1269
Size: 122 32 693 12 20 12 378
Fragments arranged by size:
693 378 122 32 20 12 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 369 1162 1269
Size: 132 237 793 107
Fragments arranged by size:
793 237 132 107
XmaI C'CCGG_G
Cuts at: 0 298 1269
Size: 298 971
XmnI GAAnn'nnTTC
Cuts at: 0 722 1208 1269
Size: 722 486 61
Fragments arranged by size:
722 486 61
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BaeI BamHI BanII
BbsI BcgI BglI Bme1580I BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsmI BsmBI
Bsp1286I BsrBI BsrFI BsrGI BssSI BstEII BstXI BstYI
BtgI BtsI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI Hin4I MscI MslI MspA1I
NaeI NgoMIV PasI PflMI PmlI PpiI PpuMI PsiI
PspOMI PstI PvuII SanDI SfcI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BanI BbeI BbvCI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI Bpu10I BsaBI BsaWI
BsgI BsiHKAI BsiWI BspEI BspHI BspMI BsrDI BssHII
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PshAI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI