(Linear) (Six-base) MAPSORT of: KIEE0419.seq  Check: 341  from: 1  to: 483



KIEE0419.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AflIII A'CryG_T

Cuts at:      0     305     307     483 
   Size:        305       2     176

  Fragments arranged by size: 

                 305     176       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     418     483 
   Size:        418      65
 
ApaLI G'TGCA_C

Cuts at:      0     311     483 
   Size:        311     172
 
AvaI C'yCGr_G

Cuts at:      0      50     483 
   Size:         50     433
 
BanI G'GyrC_C

Cuts at:      0     204     298     483 
   Size:        204      94     185

  Fragments arranged by size: 

                 204     185      94
 
Bme1580I G_kGCm'C

Cuts at:      0     301     315     483 
   Size:        301      14     168

  Fragments arranged by size: 

                 301     168      14
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     483 
   Size:        442      41
 
Bpu10I CC'TnA_GC

Cuts at:      0     209     483 
   Size:        209     274
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      51     483 
   Size:         51     432
 
BsaAI yAC'GTr

Cuts at:      0     183     308     483 
   Size:        183     125     175

  Fragments arranged by size: 

                 183     175     125
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     336     445     483 
   Size:        336     109      38

  Fragments arranged by size: 

                 336     109      38
 
BsiHKAI G_wGCw'C

Cuts at:      0     188     215     315     368     483 
   Size:        188      27     100      53     115

  Fragments arranged by size: 

                 188     115     100      53      27
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     215     301     315     368     483 
   Size:        188      27      86      14      53     115

  Fragments arranged by size: 

                 188     115      86      53      27      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      33     483 
   Size:         33     450
 
BsrBI CCG'CTC

Cuts at:      0     224     483 
   Size:        224     259
 
BssSI C'ACGA_G

Cuts at:      0     420     483 
   Size:        420      63
 
BstEII G'GTnAC_C

Cuts at:      0      63     483 
   Size:         63     420
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     325     483 
   Size:        325     158
 
BtgI C'CryG_G

Cuts at:      0     464     483 
   Size:        464      19
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       3     483 
   Size:          3     480
 
DraIII CAC_nnn'GTG

Cuts at:      0     422     483 
   Size:        422      61
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     379     483 
   Size:        379     104
 
EaeI y'GGCC_r

Cuts at:      0       8     327     483 
   Size:          8     319     156

  Fragments arranged by size: 

                 319     156       8
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     264     483 
   Size:        264     219
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     483 
   Size:        442      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     457     483 
   Size:        457      26
 
EcoO109I rG'GnC_Cy

Cuts at:      0     261     483 
   Size:        261     222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      87     119     460     483 
   Size:         87      32     341      23

  Fragments arranged by size: 

                 341      87      32      23
 
MscI TGG'CCA

Cuts at:      0      10     329     483 
   Size:         10     319     154

  Fragments arranged by size: 

                 319     154      10
 
PmlI CAC'GTG

Cuts at:      0     183     308     483 
   Size:        183     125     175

  Fragments arranged by size: 

                 183     175     125
 
PpuMI rG'GwC_Cy

Cuts at:      0     261     483 
   Size:        261     222
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     153     354     483 
   Size:        153     201     129

  Fragments arranged by size: 

                 201     153     129
 

 Enzymes that do cut:

   AflIII     AlfI    ApaLI     AvaI     BanI Bme1580I     BpmI   Bpu10I
     BsaI    BsaAI    BseRI  BsiHKAI Bsp1286I    BspMI    BsrBI    BssSI
   BstEII    BstXI     BtgI    BtgZI   DraIII     DrdI     EaeI     EciI
  Eco57MI    EcoNI EcoO109I    Hin4I     MscI     PmlI    PpuMI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI     EagI     EarI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI