Restriction map for
pF1KE0428
(Linear) (Six-base) MAPSORT of: KIEE0428.seq Check: 8828 from: 1 to: 897
KIEE0428.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 361 897
Size: 361 536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 880 897
Size: 610 270 17
Fragments arranged by size:
610 270 17
AflIII A'CryG_T
Cuts at: 0 217 509 897
Size: 217 292 388
Fragments arranged by size:
388 292 217
AlwNI CAG_nnn'CTG
Cuts at: 0 774 897
Size: 774 123
ApoI r'AATT_y
Cuts at: 0 394 897
Size: 394 503
AvaI C'yCGr_G
Cuts at: 0 149 897
Size: 149 748
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 64 97 897
Size: 64 33 800
Fragments arranged by size:
800 64 33
BamHI G'GATC_C
Cuts at: 0 653 897
Size: 653 244
BanI G'GyrC_C
Cuts at: 0 824 897
Size: 824 73
BanII G_rGCy'C
Cuts at: 0 111 897
Size: 111 786
BbeI G_GCGC'C
Cuts at: 0 828 897
Size: 828 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 725 897
Size: 725 172
BclI T'GATC_A
Cuts at: 0 666 897
Size: 666 231
BmgBI CAC'GTC
Cuts at: 0 459 897
Size: 459 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 120 897
Size: 120 777
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 897
Size: 711 186
BsaAI yAC'GTr
Cuts at: 0 220 897
Size: 220 677
BsaHI Gr'CG_yC
Cuts at: 0 825 897
Size: 825 72
BsaWI w'CCGG_w
Cuts at: 0 707 897
Size: 707 190
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 763 793 897
Size: 763 30 104
Fragments arranged by size:
763 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 897
Size: 274 623
Bsp1286I G_dGCh'C
Cuts at: 0 111 897
Size: 111 786
BspHI T'CATG_A
Cuts at: 0 608 897
Size: 608 289
BstYI r'GATC_y
Cuts at: 0 653 795 897
Size: 653 142 102
Fragments arranged by size:
653 142 102
BstZ17I GTA'TAC
Cuts at: 0 362 897
Size: 362 535
Bsu36I CC'TnA_GG
Cuts at: 0 22 897
Size: 22 875
BtgI C'CryG_G
Cuts at: 0 296 897
Size: 296 601
EaeI y'GGCC_r
Cuts at: 0 677 897
Size: 677 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 711 880 897
Size: 610 101 169 17
Fragments arranged by size:
610 169 101 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 89 897
Size: 89 808
EcoO109I rG'GnC_Cy
Cuts at: 0 310 897
Size: 310 587
EcoRI G'AATT_C
Cuts at: 0 394 897
Size: 394 503
FspI TGC'GCA
Cuts at: 0 355 897
Size: 355 542
HaeII r_GCGC'y
Cuts at: 0 828 897
Size: 828 69
HindIII A'AGCT_T
Cuts at: 0 223 897
Size: 223 674
KasI G'GCGC_C
Cuts at: 0 824 897
Size: 824 73
MslI CAynn'nnrTG
Cuts at: 0 623 897
Size: 623 274
MspA1I CmG'CkG
Cuts at: 0 195 682 897
Size: 195 487 215
Fragments arranged by size:
487 215 195
NarI GG'CG_CC
Cuts at: 0 825 897
Size: 825 72
NcoI C'CATG_G
Cuts at: 0 296 897
Size: 296 601
NspI r_CATG'y
Cuts at: 0 158 360 513 897
Size: 158 202 153 384
Fragments arranged by size:
384 202 158 153
PciI A'CATG_T
Cuts at: 0 509 897
Size: 509 388
PfoI T'CCnGG_A
Cuts at: 0 177 897
Size: 177 720
PpuMI rG'GwC_Cy
Cuts at: 0 310 897
Size: 310 587
PsiI TTA'TAA
Cuts at: 0 445 897
Size: 445 452
PvuII CAG'CTG
Cuts at: 0 195 897
Size: 195 702
SfoI GGC'GCC
Cuts at: 0 826 897
Size: 826 71
StyI C'CwwG_G
Cuts at: 0 90 296 897
Size: 90 206 601
Fragments arranged by size:
601 206 90
TatI w'GTAC_w
Cuts at: 0 506 897
Size: 506 391
XmnI GAAnn'nnTTC
Cuts at: 0 173 398 897
Size: 173 225 499
Fragments arranged by size:
499 225 173
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BclI BmgBI BmrI BpmI
BsaAI BsaHI BsaWI BsaXI BseRI Bsp1286I BspHI BstYI
BstZ17I Bsu36I BtgI EaeI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII HindIII KasI MslI MspA1I NarI NcoI
NspI PciI PfoI PpuMI PsiI PvuII SfoI StyI
TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmtI BplI Bpu10I BpuEI BsaI BsaBI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoRV FalI FseI
FspAI Hin4I HincII HpaI KpnI MfeI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI