(Linear) (Six-base) MAPSORT of: KIEE0429.seq Check: 6443 from: 1 to: 1284 KIEE0429.orf With 182 enzymes: SgfI * April 24, 2009 13:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1215 1284 Size: 1215 69 Acc65I G'GTAC_C Cuts at: 0 665 1284 Size: 665 619 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 479 1284 Size: 479 805 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1234 1266 1284 Size: 1234 32 18 Fragments arranged by size: 1234 32 18 ApaI G_GGCC'C Cuts at: 0 19 102 263 1284 Size: 19 83 161 1021 Fragments arranged by size: 1021 161 83 19 ApaLI G'TGCA_C Cuts at: 0 728 1284 Size: 728 556 AvaI C'yCGr_G Cuts at: 0 184 256 262 1284 Size: 184 72 6 1022 Fragments arranged by size: 1022 184 72 6 BanI G'GyrC_C Cuts at: 0 665 1227 1284 Size: 665 562 57 Fragments arranged by size: 665 562 57 BanII G_rGCy'C Cuts at: 0 19 102 263 343 1284 Size: 19 83 161 80 941 Fragments arranged by size: 941 161 83 80 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 689 1284 Size: 689 595 BbvCI CC'TCA_GC Cuts at: 0 1208 1284 Size: 1208 76 BclI T'GATC_A Cuts at: 0 168 1284 Size: 168 1116 BglI GCCn_nnn'nGGC Cuts at: 0 621 1284 Size: 621 663 Bme1580I G_kGCm'C Cuts at: 0 19 102 257 263 732 1232 1279 1284 Size: 19 83 155 6 469 500 47 5 Fragments arranged by size: 500 469 155 83 47 19 6 5 BmrI ACTGGGnnnn_n' Cuts at: 0 501 584 836 1284 Size: 501 83 252 448 Fragments arranged by size: 501 448 252 83 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 351 1195 1284 Size: 351 844 89 Fragments arranged by size: 844 351 89 Bpu10I CC'TnA_GC Cuts at: 0 128 903 1208 1284 Size: 128 775 305 76 Fragments arranged by size: 775 305 128 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 88 1284 Size: 88 1196 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1095 1125 1284 Size: 1095 30 159 Fragments arranged by size: 1095 159 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 426 555 969 972 1284 Size: 426 129 414 3 312 Fragments arranged by size: 426 414 312 129 3 BseYI C'CCAG_C Cuts at: 0 660 1059 1284 Size: 660 399 225 Fragments arranged by size: 660 399 225 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 330 1284 Size: 330 954 BsiHKAI G_wGCw'C Cuts at: 0 343 732 1284 Size: 343 389 552 Fragments arranged by size: 552 389 343 Bsp1286I G_dGCh'C Cuts at: 0 19 102 257 263 343 732 1232 1279 Size: 19 83 155 6 80 389 500 47 Cuts at: 1279 1284 Size: 5 Fragments arranged by size: 500 389 155 83 80 47 19 6 5 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 726 1215 1284 Size: 726 489 69 Fragments arranged by size: 726 489 69 BsrBI CCG'CTC Cuts at: 0 113 1142 1284 Size: 113 1029 142 Fragments arranged by size: 1029 142 113 BssSI C'ACGA_G Cuts at: 0 1272 1284 Size: 1272 12 BstAPI GCAn_nnn'nTGC Cuts at: 0 208 913 1227 1284 Size: 208 705 314 57 Fragments arranged by size: 705 314 208 57 BstEII G'GTnAC_C Cuts at: 0 189 1032 1284 Size: 189 843 252 Fragments arranged by size: 843 252 189 BstYI r'GATC_y Cuts at: 0 987 1249 1284 Size: 987 262 35 Fragments arranged by size: 987 262 35 Bsu36I CC'TnA_GG Cuts at: 0 482 553 1284 Size: 482 71 731 Fragments arranged by size: 731 482 71 BtgI C'CryG_G Cuts at: 0 383 1284 Size: 383 901 BtsI GCAGTG_nn' Cuts at: 0 432 730 1284 Size: 432 298 554 Fragments arranged by size: 554 432 298 DraIII CAC_nnn'GTG Cuts at: 0 509 1284 Size: 509 775 DrdI GACnn_nn'nnGTC Cuts at: 0 148 870 1284 Size: 148 722 414 Fragments arranged by size: 722 414 148 EarI CTCTTCn'nnn_ Cuts at: 0 498 1011 1284 Size: 498 513 273 Fragments arranged by size: 513 498 273 EcoICRI GAG'CTC Cuts at: 0 341 1284 Size: 341 943 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 351 479 1195 1284 Size: 351 128 716 89 Fragments arranged by size: 716 351 128 89 EcoO109I rG'GnC_Cy Cuts at: 0 15 98 99 141 180 921 967 1188 Size: 15 83 1 42 39 741 46 221 Cuts at: 1188 1284 Size: 96 Fragments arranged by size: 741 221 96 83 46 42 39 15 1 KpnI G_GTAC'C Cuts at: 0 669 1284 Size: 669 615 MspA1I CmG'CkG Cuts at: 0 441 1063 1120 1218 1284 Size: 441 622 57 98 66 Fragments arranged by size: 622 441 98 66 57 PfoI T'CCnGG_A Cuts at: 0 331 1261 1284 Size: 331 930 23 Fragments arranged by size: 930 331 23 PpuMI rG'GwC_Cy Cuts at: 0 141 180 921 1284 Size: 141 39 741 363 Fragments arranged by size: 741 363 141 39 PshAI GACnn'nnGTC Cuts at: 0 74 1284 Size: 74 1210 PspOMI G'GGCC_C Cuts at: 0 15 98 259 1284 Size: 15 83 161 1025 Fragments arranged by size: 1025 161 83 15 PstI C_TGCA'G Cuts at: 0 275 737 766 918 1119 1223 1284 Size: 275 462 29 152 201 104 61 Fragments arranged by size: 462 275 201 152 104 61 29 PvuII CAG'CTG Cuts at: 0 441 1063 1120 1218 1284 Size: 441 622 57 98 66 Fragments arranged by size: 622 441 98 66 57 SacI G_AGCT'C Cuts at: 0 343 1284 Size: 343 941 SanDI GG'GwC_CC Cuts at: 0 180 921 1284 Size: 180 741 363 Fragments arranged by size: 741 363 180 SfcI C'TryA_G Cuts at: 0 271 375 733 762 914 929 1115 1219 Size: 271 104 358 29 152 15 186 104 Cuts at: 1219 1284 Size: 65 Fragments arranged by size: 358 271 186 152 104 104 65 29 15 SmaI CCC'GGG Cuts at: 0 258 264 1284 Size: 258 6 1020 Fragments arranged by size: 1020 258 6 SmlI C'TyrA_G Cuts at: 0 103 1284 Size: 103 1181 SrfI GCCC'GGGC Cuts at: 0 258 264 1284 Size: 258 6 1020 Fragments arranged by size: 1020 258 6 StuI AGG'CCT Cuts at: 0 239 480 580 1284 Size: 239 241 100 704 Fragments arranged by size: 704 241 239 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 520 855 1284 Size: 520 335 429 Fragments arranged by size: 520 429 335 TatI w'GTAC_w Cuts at: 0 592 1284 Size: 592 692 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 27 775 807 1095 1127 1284 Size: 27 748 32 288 32 157 Fragments arranged by size: 748 288 157 32 32 27 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 160 1284 Size: 160 1124 XmaI C'CCGG_G Cuts at: 0 256 262 1284 Size: 256 6 1022 Fragments arranged by size: 1022 256 6 Enzymes that do cut: AarI Acc65I AcuI AloI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtsI DraIII DrdI EarI EcoICRI Eco57MI EcoO109I KpnI MspA1I PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SfcI SmaI SmlI SrfI StuI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI EaeI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StyI SwaI Tth111I XbaI XhoI XmnI ZraI