Restriction map for pF1KE0429
 (Linear) (Six-base) MAPSORT of: KIEE0429.seq  Check: 6443  from: 1  to: 1284



KIEE0429.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1215    1284 
   Size:       1215      69
 
Acc65I G'GTAC_C

Cuts at:      0     665    1284 
   Size:        665     619
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479    1284 
   Size:        479     805
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1234    1266    1284 
   Size:       1234      32      18

  Fragments arranged by size: 

                1234      32      18
 
ApaI G_GGCC'C

Cuts at:      0      19     102     263    1284 
   Size:         19      83     161    1021

  Fragments arranged by size: 

                1021     161      83      19
 
ApaLI G'TGCA_C

Cuts at:      0     728    1284 
   Size:        728     556
 
AvaI C'yCGr_G

Cuts at:      0     184     256     262    1284 
   Size:        184      72       6    1022

  Fragments arranged by size: 

                1022     184      72       6
 
BanI G'GyrC_C

Cuts at:      0     665    1227    1284 
   Size:        665     562      57

  Fragments arranged by size: 

                 665     562      57
 
BanII G_rGCy'C

Cuts at:      0      19     102     263     343    1284 
   Size:         19      83     161      80     941

  Fragments arranged by size: 

                 941     161      83      80      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     689    1284 
   Size:        689     595
 
BbvCI CC'TCA_GC

Cuts at:      0    1208    1284 
   Size:       1208      76
 
BclI T'GATC_A

Cuts at:      0     168    1284 
   Size:        168    1116
 
BglI GCCn_nnn'nGGC

Cuts at:      0     621    1284 
   Size:        621     663
 
Bme1580I G_kGCm'C

Cuts at:      0      19     102     257     263     732    1232    1279    1284 
   Size:         19      83     155       6     469     500      47       5

  Fragments arranged by size: 

                 500     469     155      83      47      19       6       5
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     501     584     836    1284 
   Size:        501      83     252     448

  Fragments arranged by size: 

                 501     448     252      83
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351    1195    1284 
   Size:        351     844      89

  Fragments arranged by size: 

                 844     351      89
 
Bpu10I CC'TnA_GC

Cuts at:      0     128     903    1208    1284 
   Size:        128     775     305      76

  Fragments arranged by size: 

                 775     305     128      76
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88    1284 
   Size:         88    1196
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1095    1125    1284 
   Size:       1095      30     159

  Fragments arranged by size: 

                1095     159      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     426     555     969     972    1284 
   Size:        426     129     414       3     312

  Fragments arranged by size: 

                 426     414     312     129       3
 
BseYI C'CCAG_C

Cuts at:      0     660    1059    1284 
   Size:        660     399     225

  Fragments arranged by size: 

                 660     399     225
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330    1284 
   Size:        330     954
 
BsiHKAI G_wGCw'C

Cuts at:      0     343     732    1284 
   Size:        343     389     552

  Fragments arranged by size: 

                 552     389     343
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     102     257     263     343     732    1232    1279 
   Size:         19      83     155       6      80     389     500      47

Cuts at:   1279    1284 
   Size:          5

  Fragments arranged by size: 

                 500     389     155      83      80      47      19       6
                   5
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     726    1215    1284 
   Size:        726     489      69

  Fragments arranged by size: 

                 726     489      69
 
BsrBI CCG'CTC

Cuts at:      0     113    1142    1284 
   Size:        113    1029     142

  Fragments arranged by size: 

                1029     142     113
 
BssSI C'ACGA_G

Cuts at:      0    1272    1284 
   Size:       1272      12
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     208     913    1227    1284 
   Size:        208     705     314      57

  Fragments arranged by size: 

                 705     314     208      57
 
BstEII G'GTnAC_C

Cuts at:      0     189    1032    1284 
   Size:        189     843     252

  Fragments arranged by size: 

                 843     252     189
 
BstYI r'GATC_y

Cuts at:      0     987    1249    1284 
   Size:        987     262      35

  Fragments arranged by size: 

                 987     262      35
 
Bsu36I CC'TnA_GG

Cuts at:      0     482     553    1284 
   Size:        482      71     731

  Fragments arranged by size: 

                 731     482      71
 
BtgI C'CryG_G

Cuts at:      0     383    1284 
   Size:        383     901
 
BtsI GCAGTG_nn'

Cuts at:      0     432     730    1284 
   Size:        432     298     554

  Fragments arranged by size: 

                 554     432     298
 
DraIII CAC_nnn'GTG

Cuts at:      0     509    1284 
   Size:        509     775
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     148     870    1284 
   Size:        148     722     414

  Fragments arranged by size: 

                 722     414     148
 
EarI CTCTTCn'nnn_

Cuts at:      0     498    1011    1284 
   Size:        498     513     273

  Fragments arranged by size: 

                 513     498     273
 
EcoICRI GAG'CTC

Cuts at:      0     341    1284 
   Size:        341     943
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     479    1195    1284 
   Size:        351     128     716      89

  Fragments arranged by size: 

                 716     351     128      89
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15      98      99     141     180     921     967    1188 
   Size:         15      83       1      42      39     741      46     221

Cuts at:   1188    1284 
   Size:         96

  Fragments arranged by size: 

                 741     221      96      83      46      42      39      15
                   1
 
KpnI G_GTAC'C

Cuts at:      0     669    1284 
   Size:        669     615
 
MspA1I CmG'CkG

Cuts at:      0     441    1063    1120    1218    1284 
   Size:        441     622      57      98      66

  Fragments arranged by size: 

                 622     441      98      66      57
 
PfoI T'CCnGG_A

Cuts at:      0     331    1261    1284 
   Size:        331     930      23

  Fragments arranged by size: 

                 930     331      23
 
PpuMI rG'GwC_Cy

Cuts at:      0     141     180     921    1284 
   Size:        141      39     741     363

  Fragments arranged by size: 

                 741     363     141      39
 
PshAI GACnn'nnGTC

Cuts at:      0      74    1284 
   Size:         74    1210
 
PspOMI G'GGCC_C

Cuts at:      0      15      98     259    1284 
   Size:         15      83     161    1025

  Fragments arranged by size: 

                1025     161      83      15
 
PstI C_TGCA'G

Cuts at:      0     275     737     766     918    1119    1223    1284 
   Size:        275     462      29     152     201     104      61

  Fragments arranged by size: 

                 462     275     201     152     104      61      29
 
PvuII CAG'CTG

Cuts at:      0     441    1063    1120    1218    1284 
   Size:        441     622      57      98      66

  Fragments arranged by size: 

                 622     441      98      66      57
 
SacI G_AGCT'C

Cuts at:      0     343    1284 
   Size:        343     941
 
SanDI GG'GwC_CC

Cuts at:      0     180     921    1284 
   Size:        180     741     363

  Fragments arranged by size: 

                 741     363     180
 
SfcI C'TryA_G

Cuts at:      0     271     375     733     762     914     929    1115    1219 
   Size:        271     104     358      29     152      15     186     104

Cuts at:   1219    1284 
   Size:         65

  Fragments arranged by size: 

                 358     271     186     152     104     104      65      29
                  15
 
SmaI CCC'GGG

Cuts at:      0     258     264    1284 
   Size:        258       6    1020

  Fragments arranged by size: 

                1020     258       6
 
SmlI C'TyrA_G

Cuts at:      0     103    1284 
   Size:        103    1181
 
SrfI GCCC'GGGC

Cuts at:      0     258     264    1284 
   Size:        258       6    1020

  Fragments arranged by size: 

                1020     258       6
 
StuI AGG'CCT

Cuts at:      0     239     480     580    1284 
   Size:        239     241     100     704

  Fragments arranged by size: 

                 704     241     239     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     520     855    1284 
   Size:        520     335     429

  Fragments arranged by size: 

                 520     429     335
 
TatI w'GTAC_w

Cuts at:      0     592    1284 
   Size:        592     692
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     775     807    1095    1127    1284 
   Size:         27     748      32     288      32     157

  Fragments arranged by size: 

                 748     288     157      32      32      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     160    1284 
   Size:        160    1124
 
XmaI C'CCGG_G

Cuts at:      0     256     262    1284 
   Size:        256       6    1022

  Fragments arranged by size: 

                1022     256       6
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AloI     ApaI    ApaLI     AvaI     BanI
    BanII     BbsI    BbvCI     BclI     BglI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BssSI   BstAPI   BstEII    BstYI   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EarI  EcoICRI  Eco57MI EcoO109I     KpnI
   MspA1I     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
    SanDI     SfcI     SmaI     SmlI     SrfI     StuI    TaqII     TatI
     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI    BglII     BlpI
    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI   BssHII    BstBI    BstXI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     EaeI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     StyI     SwaI  Tth111I     XbaI     XhoI     XmnI
     ZraI
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