Restriction map for
pF1KE0432
(Linear) (Six-base) MAPSORT of: KIEE0432.seq Check: 1633 from: 1 to: 1218
KIEE0432.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
Acc65I G'GTAC_C
Cuts at: 0 846 1218
Size: 846 372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 542 1218
Size: 68 474 676
Fragments arranged by size:
676 474 68
AflIII A'CryG_T
Cuts at: 0 33 829 1218
Size: 33 796 389
Fragments arranged by size:
796 389 33
AlwNI CAG_nnn'CTG
Cuts at: 0 649 706 1218
Size: 649 57 512
Fragments arranged by size:
649 512 57
ApoI r'AATT_y
Cuts at: 0 66 359 676 776 914 1218
Size: 66 293 317 100 138 304
Fragments arranged by size:
317 304 293 138 100 66
AvaI C'yCGr_G
Cuts at: 0 725 1218
Size: 725 493
BanI G'GyrC_C
Cuts at: 0 846 1218
Size: 846 372
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 1218
Size: 359 859
BclI T'GATC_A
Cuts at: 0 489 1218
Size: 489 729
BfrBI ATG'CAT
Cuts at: 0 903 1218
Size: 903 315
BglI GCCn_nnn'nGGC
Cuts at: 0 727 736 1218
Size: 727 9 482
Fragments arranged by size:
727 482 9
Bme1580I G_kGCm'C
Cuts at: 0 520 1069 1218
Size: 520 549 149
Fragments arranged by size:
549 520 149
BmgBI CAC'GTC
Cuts at: 0 34 1218
Size: 34 1184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1218
Size: 1045 173
Bpu10I CC'TnA_GC
Cuts at: 0 354 1218
Size: 354 864
BsaAI yAC'GTr
Cuts at: 0 275 1218
Size: 275 943
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 706 736 1218
Size: 706 30 482
Fragments arranged by size:
706 482 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 117 1218
Size: 117 1101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 692 1218
Size: 376 316 526
Fragments arranged by size:
526 376 316
BsmI GAATG_Cn'
Cuts at: 0 457 1218
Size: 457 761
BsmBI CGTCTCn'nnnn_
Cuts at: 0 24 1218
Size: 24 1194
Bsp1286I G_dGCh'C
Cuts at: 0 520 1069 1218
Size: 520 549 149
Fragments arranged by size:
549 520 149
BsrBI CCG'CTC
Cuts at: 0 111 150 1218
Size: 111 39 1068
Fragments arranged by size:
1068 111 39
BsrDI GCAATG_nn'
Cuts at: 0 47 1218
Size: 47 1171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 706 1218
Size: 706 512
BtgI C'CryG_G
Cuts at: 0 1157 1218
Size: 1157 61
DraIII CAC_nnn'GTG
Cuts at: 0 948 1218
Size: 948 270
EaeI y'GGCC_r
Cuts at: 0 623 1218
Size: 623 595
EarI CTCTTCn'nnn_
Cuts at: 0 87 94 506 659 677 1218
Size: 87 7 412 153 18 541
Fragments arranged by size:
541 412 153 87 18 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 542 1045 1218
Size: 68 474 503 173
Fragments arranged by size:
503 474 173 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 134 166 486 518 706 738 1218
Size: 134 32 320 32 188 32 480
Fragments arranged by size:
480 320 188 134 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1042 1218
Size: 1042 176
KpnI G_GTAC'C
Cuts at: 0 850 1218
Size: 850 368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 1218
Size: 744 474
MscI TGG'CCA
Cuts at: 0 625 1218
Size: 625 593
MslI CAynn'nnrTG
Cuts at: 0 59 235 1218
Size: 59 176 983
Fragments arranged by size:
983 176 59
MspA1I CmG'CkG
Cuts at: 0 1191 1218
Size: 1191 27
NcoI C'CATG_G
Cuts at: 0 1157 1218
Size: 1157 61
NsiI A_TGCA'T
Cuts at: 0 905 1218
Size: 905 313
NspI r_CATG'y
Cuts at: 0 579 700 1218
Size: 579 121 518
Fragments arranged by size:
579 518 121
PfoI T'CCnGG_A
Cuts at: 0 1093 1218
Size: 1093 125
PsiI TTA'TAA
Cuts at: 0 925 1218
Size: 925 293
PstI C_TGCA'G
Cuts at: 0 1004 1218
Size: 1004 214
SfcI C'TryA_G
Cuts at: 0 1000 1218
Size: 1000 218
SmaI CCC'GGG
Cuts at: 0 727 1218
Size: 727 491
SnaBI TAC'GTA
Cuts at: 0 275 1218
Size: 275 943
SpeI A'CTAG_T
Cuts at: 0 750 1218
Size: 750 468
SspI AAT'ATT
Cuts at: 0 129 1218
Size: 129 1089
StyI C'CwwG_G
Cuts at: 0 1157 1218
Size: 1157 61
TatI w'GTAC_w
Cuts at: 0 753 1218
Size: 753 465
XmaI C'CCGG_G
Cuts at: 0 725 1218
Size: 725 493
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApoI AvaI BanI BbsI
BclI BfrBI BglI Bme1580I BmgBI BpmI Bpu10I BsaAI
BsaXI BseRI BsgI BsmI BsmBI Bsp1286I BsrBI BsrDI
BstAPI BtgI DraIII EaeI EarI Eco57MI Hin4I HindIII
KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI
PfoI PsiI PstI SfcI SmaI SnaBI SpeI SspI
StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BglII BlpI BmrI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BspEI BspHI
BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI