(Linear) (Six-base) MAPSORT of: KIEE0433.seq  Check: 3683  from: 1  to: 1242



KIEE0433.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AatII G_ACGT'C

Cuts at:      0     976    1242 
   Size:        976     266
 
AclI AA'CG_TT

Cuts at:      0    1161    1242 
   Size:       1161      81
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     667    1242 
   Size:        306     361     575

  Fragments arranged by size: 

                 575     361     306
 
AhdI GACnn_n'nnGTC

Cuts at:      0     828    1242 
   Size:        828     414
 
AlwNI CAG_nnn'CTG

Cuts at:      0     919    1242 
   Size:        919     323
 
BanI G'GyrC_C

Cuts at:      0     289    1242 
   Size:        289     953
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     312    1242 
   Size:        312     930
 
BglII A'GATC_T

Cuts at:      0     728    1242 
   Size:        728     514
 
BmgBI CAC'GTC

Cuts at:      0     825    1242 
   Size:        825     417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     705    1242 
   Size:        705     537
 
Bpu10I CC'TnA_GC

Cuts at:      0    1229    1242 
   Size:       1229      13
 
BsaAI yAC'GTr

Cuts at:      0     102    1242 
   Size:        102    1140
 
BsaHI Gr'CG_yC

Cuts at:      0     209     973    1242 
   Size:        209     764     269

  Fragments arranged by size: 

                 764     269     209
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     213     243     244     274    1242 
   Size:        213      30       1      30     968

  Fragments arranged by size: 

                 968     213      30      30       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265    1242 
   Size:        265     977
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     847    1242 
   Size:        847     395
 
BsiHKAI G_wGCw'C

Cuts at:      0     631    1242 
   Size:        631     611
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     964    1242 
   Size:        964     278
 
Bsp1286I G_dGCh'C

Cuts at:      0     631    1242 
   Size:        631     611
 
BspHI T'CATG_A

Cuts at:      0    1112    1242 
   Size:       1112     130
 
BsrDI GCAATG_nn'

Cuts at:      0     986    1242 
   Size:        986     256
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     919    1242 
   Size:        919     323
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     853     882    1125    1242 
   Size:        853      29     243     117

  Fragments arranged by size: 

                 853     243     117      29
 
BstYI r'GATC_y

Cuts at:      0     111     728    1242 
   Size:        111     617     514

  Fragments arranged by size: 

                 617     514     111
 
BtgI C'CryG_G

Cuts at:      0     536     924    1242 
   Size:        536     388     318

  Fragments arranged by size: 

                 536     388     318
 
DraI TTT'AAA

Cuts at:      0     669    1242 
   Size:        669     573
 
DraIII CAC_nnn'GTG

Cuts at:      0     475    1242 
   Size:        475     767
 
EarI CTCTTCn'nnn_

Cuts at:      0     235    1242 
   Size:        235    1007
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     667    1242 
   Size:        306     361     575

  Fragments arranged by size: 

                 575     361     306
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     534     566     779     811     814     846    1242 
   Size:        534      32     213      32       3      32     396

  Fragments arranged by size: 

                 534     396     213      32      32      32       3
 
HindIII A'AGCT_T

Cuts at:      0    1033    1242 
   Size:       1033     209
 
MfeI C'AATT_G

Cuts at:      0     563    1097    1242 
   Size:        563     534     145

  Fragments arranged by size: 

                 563     534     145
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     812    1242 
   Size:        812     430
 
MslI CAynn'nnrTG

Cuts at:      0     122     401     541    1242 
   Size:        122     279     140     701

  Fragments arranged by size: 

                 701     279     140     122
 
NcoI C'CATG_G

Cuts at:      0     536    1242 
   Size:        536     706
 
NspI r_CATG'y

Cuts at:      0     954    1242 
   Size:        954     288
 
PstI C_TGCA'G

Cuts at:      0     183    1242 
   Size:        183    1059
 
SexAI A'CCwGG_T

Cuts at:      0     500    1242 
   Size:        500     742
 
SfcI C'TryA_G

Cuts at:      0     179    1242 
   Size:        179    1063
 
SspI AAT'ATT

Cuts at:      0     303    1242 
   Size:        303     939
 
StyI C'CwwG_G

Cuts at:      0     170     536     846    1118    1242 
   Size:        170     366     310     272     124

  Fragments arranged by size: 

                 366     310     272     170     124
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     298    1242 
   Size:        298     944
 
TatI w'GTAC_w

Cuts at:      0     701    1242 
   Size:        701     541
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     213     245    1242 
   Size:        213      32     997

  Fragments arranged by size: 

                 997     213      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     163    1242 
   Size:        163    1079
 
ZraI GAC'GTC

Cuts at:      0     974    1242 
   Size:        974     268
 

 Enzymes that do cut:

    AatII     AclI     AcuI     AhdI    AlwNI     BanI     BbsI    BglII
    BmgBI     BmrI   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspHI    BsrDI   BstAPI    BstXI    BstYI
     BtgI     DraI   DraIII     EarI  Eco57MI    Hin4I  HindIII     MfeI
     MmeI     MslI     NcoI     NspI     PstI    SexAI     SfcI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaWI    BseYI    BsiEI    BsiWI
     BsmI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI     MscI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     XbaI     XcmI     XhoI     XmaI     XmnI