(Linear) (Six-base) MAPSORT of: KIEE0435.seq  Check: 4178  from: 1  to: 771



KIEE0435.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     596     771 
   Size:        596     175
 
AfeI AGC'GCT

Cuts at:      0     763     771 
   Size:        763       8
 
BanI G'GyrC_C

Cuts at:      0     670     771 
   Size:        670     101
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      57     771 
   Size:         57     714
 
Bme1580I G_kGCm'C

Cuts at:      0     673     771 
   Size:        673      98
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750     771 
   Size:        750      21
 
BsaHI Gr'CG_yC

Cuts at:      0     640     771 
   Size:        640     131
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     215     245     748     771 
   Size:        215      30     503      23

  Fragments arranged by size: 

                 503     215      30      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     446     771 
   Size:        446     325
 
BsiEI CG_ry'CG

Cuts at:      0      11     771 
   Size:         11     760
 
BsiHKAI G_wGCw'C

Cuts at:      0     665     771 
   Size:        665     106
 
BsmI GAATG_Cn'

Cuts at:      0     440     679     771 
   Size:        440     239      92

  Fragments arranged by size: 

                 440     239      92
 
Bsp1286I G_dGCh'C

Cuts at:      0     665     673     771 
   Size:        665       8      98

  Fragments arranged by size: 

                 665      98       8
 
BspHI T'CATG_A

Cuts at:      0     377     482     771 
   Size:        377     105     289

  Fragments arranged by size: 

                 377     289     105
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     596     771 
   Size:        596     175
 
BsrDI GCAATG_nn'

Cuts at:      0      32     771 
   Size:         32     739
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      57      90     771 
   Size:         57      33     681

  Fragments arranged by size: 

                 681      57      33
 
EaeI y'GGCC_r

Cuts at:      0       8     771 
   Size:          8     763
 
EagI C'GGCC_G

Cuts at:      0       8     771 
   Size:          8     763
 
EarI CTCTTCn'nnn_

Cuts at:      0     298     771 
   Size:        298     473
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     750     771 
   Size:        750      21
 
HaeII r_GCGC'y

Cuts at:      0     765     771 
   Size:        765       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     215     247     771 
   Size:        215      32     524

  Fragments arranged by size: 

                 524     215      32
 
MspA1I CmG'CkG

Cuts at:      0     701     771 
   Size:        701      70
 
NspI r_CATG'y

Cuts at:      0     511     771 
   Size:        511     260
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     591     771 
   Size:        591     180
 
PshAI GACnn'nnGTC

Cuts at:      0     490     771 
   Size:        490     281
 
PsiI TTA'TAA

Cuts at:      0     272     652     771 
   Size:        272     380     119

  Fragments arranged by size: 

                 380     272     119
 
PvuII CAG'CTG

Cuts at:      0     701     771 
   Size:        701      70
 
StyI C'CwwG_G

Cuts at:      0     419     771 
   Size:        419     352
 

 Enzymes that do cut:

     AarI     AfeI     BanI     BbsI Bme1580I     BpmI    BsaHI    BsaXI
    BseRI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BtgZI     EaeI     EagI     EarI  Eco57MI    HaeII    Hin4I   MspA1I
     NspI    PflMI    PshAI     PsiI    PvuII     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BseYI     BsgI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI