Restriction map for
pF1KE0437
(Linear) (Six-base) MAPSORT of: KIEE0437.seq Check: 627 from: 1 to: 930
KIEE0437.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 5 930
Size: 5 925
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 930
Size: 410 520
AhdI GACnn_n'nnGTC
Cuts at: 0 888 930
Size: 888 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 440 930
Size: 440 490
AlwNI CAG_nnn'CTG
Cuts at: 0 60 157 930
Size: 60 97 773
Fragments arranged by size:
773 97 60
AvaI C'yCGr_G
Cuts at: 0 378 930
Size: 378 552
BanII G_rGCy'C
Cuts at: 0 567 698 930
Size: 567 131 232
Fragments arranged by size:
567 232 131
BbsI GAAGACnn'nnnn_
Cuts at: 0 870 930
Size: 870 60
BclI T'GATC_A
Cuts at: 0 734 930
Size: 734 196
BfrBI ATG'CAT
Cuts at: 0 757 930
Size: 757 173
BlpI GC'TnA_GC
Cuts at: 0 428 635 930
Size: 428 207 295
Fragments arranged by size:
428 295 207
Bme1580I G_kGCm'C
Cuts at: 0 385 930
Size: 385 545
BmrI ACTGGGnnnn_n'
Cuts at: 0 193 930
Size: 193 737
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 618 930
Size: 618 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 581 930
Size: 443 138 349
Fragments arranged by size:
443 349 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 930
Size: 437 493
BsaI GGTCTCn'nnnn_
Cuts at: 0 703 930
Size: 703 227
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 57 87 117 145 147 175 517 547
Size: 57 30 30 28 2 28 342 30
Cuts at: 547 549 579 910 930
Size: 2 30 331 20
Fragments arranged by size:
342 331 57 30 30 30 30 28
28 20 2 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 607 930
Size: 277 330 323
Fragments arranged by size:
330 323 277
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 930
Size: 557 373
BsiHKAI G_wGCw'C
Cuts at: 0 197 567 698 930
Size: 197 370 131 232
Fragments arranged by size:
370 232 197 131
BsmI GAATG_Cn'
Cuts at: 0 849 930
Size: 849 81
Bsp1286I G_dGCh'C
Cuts at: 0 197 385 567 698 930
Size: 197 188 182 131 232
Fragments arranged by size:
232 197 188 182 131
BsrDI GCAATG_nn'
Cuts at: 0 797 930
Size: 797 133
BstAPI GCAn_nnn'nTGC
Cuts at: 0 157 930
Size: 157 773
BstBI TT'CG_AA
Cuts at: 0 283 930
Size: 283 647
BstXI CCAn_nnnn'nTGG
Cuts at: 0 559 930
Size: 559 371
Bsu36I CC'TnA_GG
Cuts at: 0 497 930
Size: 497 433
BtgI C'CryG_G
Cuts at: 0 803 930
Size: 803 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 759 930
Size: 759 171
BtsI GCAGTG_nn'
Cuts at: 0 155 930
Size: 155 775
DrdI GACnn_nn'nnGTC
Cuts at: 0 594 930
Size: 594 336
EarI CTCTTCn'nnn_
Cuts at: 0 22 95 254 930
Size: 22 73 159 676
Fragments arranged by size:
676 159 73 22
EcoICRI GAG'CTC
Cuts at: 0 565 696 930
Size: 565 131 234
Fragments arranged by size:
565 234 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 443 581 930
Size: 410 33 138 349
Fragments arranged by size:
410 349 138 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 392 726 930
Size: 392 334 204
Fragments arranged by size:
392 334 204
EcoO109I rG'GnC_Cy
Cuts at: 0 890 930
Size: 890 40
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 33 930
Size: 33 897
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 117 145 149 177 874 906 930
Size: 117 28 4 28 697 32 24
Fragments arranged by size:
697 117 32 28 28 24 4
HincII GTy'rAC
Cuts at: 0 332 930
Size: 332 598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 194 592 930
Size: 118 76 398 338
Fragments arranged by size:
398 338 118 76
MslI CAynn'nnrTG
Cuts at: 0 703 787 802 930
Size: 703 84 15 128
Fragments arranged by size:
703 128 84 15
MspA1I CmG'CkG
Cuts at: 0 424 634 930
Size: 424 210 296
Fragments arranged by size:
424 296 210
NsiI A_TGCA'T
Cuts at: 0 759 930
Size: 759 171
NspI r_CATG'y
Cuts at: 0 628 757 930
Size: 628 129 173
Fragments arranged by size:
628 173 129
PasI CC'CwG_GG
Cuts at: 0 728 930
Size: 728 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 122 930
Size: 122 808
PpuMI rG'GwC_Cy
Cuts at: 0 890 930
Size: 890 40
PvuII CAG'CTG
Cuts at: 0 634 930
Size: 634 296
SacI G_AGCT'C
Cuts at: 0 567 698 930
Size: 567 131 232
Fragments arranged by size:
567 232 131
SmlI C'TyrA_G
Cuts at: 0 452 930
Size: 452 478
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 565 630 930
Size: 565 65 300
Fragments arranged by size:
565 300 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 549 581 708 740 930
Size: 549 32 127 32 190
Fragments arranged by size:
549 190 127 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 284 930
Size: 284 646
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI AvaI BanII BbsI
BclI BfrBI BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrDI
BstAPI BstBI BstXI Bsu36I BtgI BtgZI BtsI DrdI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII
MmeI MslI MspA1I NsiI NspI PasI PflMI PpuMI
PvuII SacI SmlI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BglI BglII BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstEII BstYI BstZ17I
ClaI CspCI DraI DraIII EaeI EagI EciI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI