(Linear) (Six-base) MAPSORT of: KIEE0440.seq  Check: 3410  from: 1  to: 918



KIEE0440.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     906     918 
   Size:        906      12
 
AccI GT'mk_AC

Cuts at:      0     491     918 
   Size:        491     427
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     223     918 
   Size:        131      92     695

  Fragments arranged by size: 

                 695     131      92
 
AhdI GACnn_n'nnGTC

Cuts at:      0     389     918 
   Size:        389     529
 
ApoI r'AATT_y

Cuts at:      0     323     428     918 
   Size:        323     105     490

  Fragments arranged by size: 

                 490     323     105
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     384     918 
   Size:        384     534
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       1      35     918 
   Size:          1      34     883

  Fragments arranged by size: 

                 883      34       1
 
BclI T'GATC_A

Cuts at:      0     291     864     918 
   Size:        291     573      54

  Fragments arranged by size: 

                 573     291      54
 
BfrBI ATG'CAT

Cuts at:      0      67     918 
   Size:         67     851
 
BmgBI CAC'GTC

Cuts at:      0     391     918 
   Size:        391     527
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     648     918 
   Size:        648     270
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     559     918 
   Size:        559     359
 
Bpu10I CC'TnA_GC

Cuts at:      0     731     918 
   Size:        731     187
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     918 
   Size:        115     803
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     906     918 
   Size:        906      12
 
BsrGI T'GTAC_A

Cuts at:      0     520     918 
   Size:        520     398
 
Bsu36I CC'TnA_GG

Cuts at:      0     310     918 
   Size:        310     608
 
DraI TTT'AAA

Cuts at:      0     482     918 
   Size:        482     436
 
EarI CTCTTCn'nnn_

Cuts at:      0      38     417     771     918 
   Size:         38     379     354     147

  Fragments arranged by size: 

                 379     354     147      38
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     223     918 
   Size:        131      92     695

  Fragments arranged by size: 

                 695     131      92
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      35     371     547     918 
   Size:         35     336     176     371

  Fragments arranged by size: 

                 371     336     176      35
 
EcoRV GAT'ATC

Cuts at:      0     264     918 
   Size:        264     654
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     401     918 
   Size:        401     517
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     375     407     541     573     877     909 
   Size:        112      32     231      32     134      32     304      32

Cuts at:    909     918 
   Size:          9

  Fragments arranged by size: 

                 304     231     134     112      32      32      32      32
                   9
 
HincII GTy'rAC

Cuts at:      0     276     918 
   Size:        276     642
 
HpaI GTT'AAC

Cuts at:      0     276     918 
   Size:        276     642
 
MspA1I CmG'CkG

Cuts at:      0     469     661     918 
   Size:        469     192     257

  Fragments arranged by size: 

                 469     257     192
 
NsiI A_TGCA'T

Cuts at:      0      69     918 
   Size:         69     849
 
NspI r_CATG'y

Cuts at:      0      67     918 
   Size:         67     851
 
PfoI T'CCnGG_A

Cuts at:      0      36     918 
   Size:         36     882
 
PvuII CAG'CTG

Cuts at:      0     469     661     918 
   Size:        469     192     257

  Fragments arranged by size: 

                 469     257     192
 
ScaI AGT'ACT

Cuts at:      0     244     918 
   Size:        244     674
 
SfcI C'TryA_G

Cuts at:      0     662     918 
   Size:        662     256
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     367     509     918 
   Size:        367     142     409

  Fragments arranged by size: 

                 409     367     142
 
TatI w'GTAC_w

Cuts at:      0      60     157     242     520     918 
   Size:         60      97      85     278     398

  Fragments arranged by size: 

                 398     278      97      85      60
 
XmnI GAAnn'nnTTC

Cuts at:      0      78     918 
   Size:         78     840
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     ApoI     BbsI     BcgI     BclI
    BfrBI    BmgBI     BmrI     BplI   Bpu10I    BseRI    BspMI    BsrGI
   Bsu36I     DraI     EarI  Eco57MI    EcoNI    EcoRV     FalI    Hin4I
   HincII     HpaI   MspA1I     NsiI     NspI     PfoI    PvuII     ScaI
     SfcI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
    BciVI     BglI    BglII     BlpI Bme1580I     BmtI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI