Restriction map for
pF1KE0445
(Linear) (Six-base) MAPSORT of: KIEE0445.seq Check: 9258 from: 1 to: 960
KIEE0445.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 734 960
Size: 734 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 960
Size: 68 892
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 60 92 960
Size: 60 32 868
Fragments arranged by size:
868 60 32
AlwNI CAG_nnn'CTG
Cuts at: 0 163 960
Size: 163 797
ApaLI G'TGCA_C
Cuts at: 0 554 751 960
Size: 554 197 209
Fragments arranged by size:
554 209 197
BanI G'GyrC_C
Cuts at: 0 260 280 960
Size: 260 20 680
Fragments arranged by size:
680 260 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 953 960
Size: 953 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 365 960
Size: 365 595
BclI T'GATC_A
Cuts at: 0 386 960
Size: 386 574
BglI GCCn_nnn'nGGC
Cuts at: 0 17 960
Size: 17 943
BglII A'GATC_T
Cuts at: 0 522 960
Size: 522 438
Bme1580I G_kGCm'C
Cuts at: 0 131 285 558 755 960
Size: 131 154 273 197 205
Fragments arranged by size:
273 205 197 154 131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 896 960
Size: 539 357 64
Fragments arranged by size:
539 357 64
Bpu10I CC'TnA_GC
Cuts at: 0 865 960
Size: 865 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 776 960
Size: 776 184
BsaBI GATnn'nnATC
Cuts at: 0 388 960
Size: 388 572
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 960
Size: 4 30 926
Fragments arranged by size:
926 30 4
BseYI C'CCAG_C
Cuts at: 0 136 960
Size: 136 824
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 745 960
Size: 588 157 215
Fragments arranged by size:
588 215 157
BsiHKAI G_wGCw'C
Cuts at: 0 558 755 960
Size: 558 197 205
Fragments arranged by size:
558 205 197
Bsp1286I G_dGCh'C
Cuts at: 0 131 285 558 755 960
Size: 131 154 273 197 205
Fragments arranged by size:
273 205 197 154 131
BspHI T'CATG_A
Cuts at: 0 104 960
Size: 104 856
BsrDI GCAATG_nn'
Cuts at: 0 131 960
Size: 131 829
BstAPI GCAn_nnn'nTGC
Cuts at: 0 759 960
Size: 759 201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 903 960
Size: 903 57
BstYI r'GATC_y
Cuts at: 0 272 368 522 960
Size: 272 96 154 438
Fragments arranged by size:
438 272 154 96
BtsI GCAGTG_nn'
Cuts at: 0 6 413 753 960
Size: 6 407 340 207
Fragments arranged by size:
407 340 207 6
EaeI y'GGCC_r
Cuts at: 0 413 456 632 960
Size: 413 43 176 328
Fragments arranged by size:
413 328 176 43
EarI CTCTTCn'nnn_
Cuts at: 0 211 960
Size: 211 749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 539 896 960
Size: 68 471 357 64
Fragments arranged by size:
471 357 68 64
EcoO109I rG'GnC_Cy
Cuts at: 0 924 960
Size: 924 36
FspI TGC'GCA
Cuts at: 0 405 960
Size: 405 555
HaeII r_GCGC'y
Cuts at: 0 348 960
Size: 348 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 257 289 894 926 960
Size: 257 32 605 32 34
Fragments arranged by size:
605 257 34 32 32
HincII GTy'rAC
Cuts at: 0 735 918 960
Size: 735 183 42
Fragments arranged by size:
735 183 42
HindIII A'AGCT_T
Cuts at: 0 873 960
Size: 873 87
MscI TGG'CCA
Cuts at: 0 458 634 960
Size: 458 176 326
Fragments arranged by size:
458 326 176
MslI CAynn'nnrTG
Cuts at: 0 103 255 307 901 960
Size: 103 152 52 594 59
Fragments arranged by size:
594 152 103 59 52
MspA1I CmG'CkG
Cuts at: 0 401 960
Size: 401 559
NspI r_CATG'y
Cuts at: 0 341 514 960
Size: 341 173 446
Fragments arranged by size:
446 341 173
PpuMI rG'GwC_Cy
Cuts at: 0 924 960
Size: 924 36
PvuII CAG'CTG
Cuts at: 0 401 960
Size: 401 559
SalI G'TCGA_C
Cuts at: 0 733 960
Size: 733 227
SfcI C'TryA_G
Cuts at: 0 812 960
Size: 812 148
SphI G_CATG'C
Cuts at: 0 514 960
Size: 514 446
StyI C'CwwG_G
Cuts at: 0 276 453 960
Size: 276 177 507
Fragments arranged by size:
507 276 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 297 960
Size: 112 185 663
Fragments arranged by size:
663 185 112
TatI w'GTAC_w
Cuts at: 0 423 474 960
Size: 423 51 486
Fragments arranged by size:
486 423 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4 36 960
Size: 4 32 924
Fragments arranged by size:
924 32 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 13 405 451 627 672 960
Size: 13 392 46 176 45 288
Fragments arranged by size:
392 288 176 46 45 13
Enzymes that do cut:
AccI AcuI AloI AlwNI ApaLI BanI BbsI BciVI
BclI BglI BglII Bme1580I BpmI Bpu10I BsaI BsaBI
BsaXI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrDI BstAPI
BstXI BstYI BtsI EaeI EarI Eco57MI EcoO109I FspI
HaeII Hin4I HincII HindIII MscI MslI MspA1I NspI
PpuMI PvuII SalI SfcI SphI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BfrBI BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaHI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI