(Linear) (Six-base) MAPSORT of: KIEE0447.seq  Check: 1496  from: 1  to: 525



KIEE0447.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AatII G_ACGT'C

Cuts at:      0     121     525 
   Size:        121     404
 
AccI GT'mk_AC

Cuts at:      0     121     383     525 
   Size:        121     262     142

  Fragments arranged by size: 

                 262     142     121
 
BamHI G'GATC_C

Cuts at:      0      26     368     525 
   Size:         26     342     157

  Fragments arranged by size: 

                 342     157      26
 
BanI G'GyrC_C

Cuts at:      0     168     525 
   Size:        168     357
 
Bme1580I G_kGCm'C

Cuts at:      0     171     525 
   Size:        171     354
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     525 
   Size:        327     198
 
Bpu10I CC'TnA_GC

Cuts at:      0     464     525 
   Size:        464      61
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     459     525 
   Size:        459      66
 
BsaHI Gr'CG_yC

Cuts at:      0     118     525 
   Size:        118     407
 
BsaWI w'CCGG_w

Cuts at:      0     365     525 
   Size:        365     160
 
BseYI C'CCAG_C

Cuts at:      0      58     172     372     525 
   Size:         58     114     200     153

  Fragments arranged by size: 

                 200     153     114      58
 
BsiHKAI G_wGCw'C

Cuts at:      0      92     525 
   Size:         92     433
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     171     525 
   Size:         92      79     354

  Fragments arranged by size: 

                 354      92      79
 
BstYI r'GATC_y

Cuts at:      0      26     368     525 
   Size:         26     342     157

  Fragments arranged by size: 

                 342     157      26
 
EaeI y'GGCC_r

Cuts at:      0     148     525 
   Size:        148     377
 
EarI CTCTTCn'nnn_

Cuts at:      0     308     485     525 
   Size:        308     177      40

  Fragments arranged by size: 

                 308     177      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     525 
   Size:         19     506
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     525 
   Size:        327     198
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     211     525 
   Size:        211     314
 
EcoO109I rG'GnC_Cy

Cuts at:      0     423     525 
   Size:        423     102
 
HaeII r_GCGC'y

Cuts at:      0     321     525 
   Size:        321     204
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     208     240     525 
   Size:        208      32     285

  Fragments arranged by size: 

                 285     208      32
 
HincII GTy'rAC

Cuts at:      0     231     525 
   Size:        231     294
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     525 
   Size:         77     448
 
MspA1I CmG'CkG

Cuts at:      0     176     376     525 
   Size:        176     200     149

  Fragments arranged by size: 

                 200     176     149
 
PpuMI rG'GwC_Cy

Cuts at:      0     423     525 
   Size:        423     102
 
PvuII CAG'CTG

Cuts at:      0     176     376     525 
   Size:        176     200     149

  Fragments arranged by size: 

                 200     176     149
 
SapI GCTCTTCn'nnn_

Cuts at:      0     308     525 
   Size:        308     217
 
StyI C'CwwG_G

Cuts at:      0     442     525 
   Size:        442      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     525 
   Size:        186     339
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     143     175     525 
   Size:        143      32     350

  Fragments arranged by size: 

                 350     143      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     328     525 
   Size:        328     197
 
XmnI GAAnn'nnTTC

Cuts at:      0     247     525 
   Size:        247     278
 
ZraI GAC'GTC

Cuts at:      0     119     525 
   Size:        119     406
 

 Enzymes that do cut:

    AatII     AccI    BamHI     BanI Bme1580I     BpmI   Bpu10I     BsaI
    BsaHI    BsaWI    BseYI  BsiHKAI Bsp1286I    BstYI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     MmeI
   MspA1I    PpuMI    PvuII     SapI     StyI    TaqII     TstI     XcmI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmaI