Restriction map for
pF1KE0450
(Linear) (Six-base) MAPSORT of: KIEE0450.seq Check: 5724 from: 1 to: 1344
KIEE0450.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 435 703 1344
Size: 435 268 641
Fragments arranged by size:
641 435 268
AatII G_ACGT'C
Cuts at: 0 755 1344
Size: 755 589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 714 969 1153 1344
Size: 580 134 255 184 191
Fragments arranged by size:
580 255 191 184 134
AflII C'TTAA_G
Cuts at: 0 75 1344
Size: 75 1269
AflIII A'CryG_T
Cuts at: 0 1126 1344
Size: 1126 218
AleI CACnn'nnGTG
Cuts at: 0 337 1344
Size: 337 1007
AlwNI CAG_nnn'CTG
Cuts at: 0 615 963 1344
Size: 615 348 381
Fragments arranged by size:
615 381 348
ApaI G_GGCC'C
Cuts at: 0 126 1344
Size: 126 1218
AvaI C'yCGr_G
Cuts at: 0 550 1344
Size: 550 794
BanI G'GyrC_C
Cuts at: 0 297 1086 1279 1344
Size: 297 789 193 65
Fragments arranged by size:
789 297 193 65
BanII G_rGCy'C
Cuts at: 0 126 569 580 1344
Size: 126 443 11 764
Fragments arranged by size:
764 443 126 11
BbeI G_GCGC'C
Cuts at: 0 301 1344
Size: 301 1043
BbsI GAAGACnn'nnnn_
Cuts at: 0 645 809 1029 1344
Size: 645 164 220 315
Fragments arranged by size:
645 315 220 164
BbvCI CC'TCA_GC
Cuts at: 0 148 431 451 1344
Size: 148 283 20 893
Fragments arranged by size:
893 283 148 20
BclI T'GATC_A
Cuts at: 0 929 1344
Size: 929 415
BglI GCCn_nnn'nGGC
Cuts at: 0 373 463 605 1252 1344
Size: 373 90 142 647 92
Fragments arranged by size:
647 373 142 92 90
BlpI GC'TnA_GC
Cuts at: 0 841 1273 1344
Size: 841 432 71
Fragments arranged by size:
841 432 71
Bme1580I G_kGCm'C
Cuts at: 0 126 869 1091 1344
Size: 126 743 222 253
Fragments arranged by size:
743 253 222 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 191 1330 1344
Size: 191 1139 14
Fragments arranged by size:
1139 191 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 1344
Size: 249 1095
Bpu10I CC'TnA_GC
Cuts at: 0 148 313 431 451 1344
Size: 148 165 118 20 893
Fragments arranged by size:
893 165 148 118 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 1344
Size: 216 1128
BsaI GGTCTCn'nnnn_
Cuts at: 0 426 1344
Size: 426 918
BsaAI yAC'GTr
Cuts at: 0 864 1127 1344
Size: 864 263 217
Fragments arranged by size:
864 263 217
BsaHI Gr'CG_yC
Cuts at: 0 298 365 752 1344
Size: 298 67 387 592
Fragments arranged by size:
592 387 298 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 1231 1261 1344
Size: 214 30 987 30 83
Fragments arranged by size:
987 214 83 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 988 1344
Size: 409 579 356
Fragments arranged by size:
579 409 356
BseYI C'CCAG_C
Cuts at: 0 157 581 594 956 1148 1344
Size: 157 424 13 362 192 196
Fragments arranged by size:
424 362 196 192 157 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 1344
Size: 1201 143
BsiHKAI G_wGCw'C
Cuts at: 0 998 1344
Size: 998 346
BsmBI CGTCTCn'nnnn_
Cuts at: 0 847 1344
Size: 847 497
Bsp1286I G_dGCh'C
Cuts at: 0 126 569 580 869 998 1091 1344
Size: 126 443 11 289 129 93 253
Fragments arranged by size:
443 289 253 129 126 93 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 435 503 703 960 1344
Size: 435 68 200 257 384
Fragments arranged by size:
435 384 257 200 68
BsrBI CCG'CTC
Cuts at: 0 399 1277 1344
Size: 399 878 67
Fragments arranged by size:
878 399 67
BsrDI GCAATG_nn'
Cuts at: 0 899 1344
Size: 899 445
BssSI C'ACGA_G
Cuts at: 0 358 1344
Size: 358 986
BstEII G'GTnAC_C
Cuts at: 0 318 1344
Size: 318 1026
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 672 882 918 1071 1344
Size: 594 78 210 36 153 273
Fragments arranged by size:
594 273 210 153 78 36
BstYI r'GATC_y
Cuts at: 0 878 1344
Size: 878 466
Bsu36I CC'TnA_GG
Cuts at: 0 236 1344
Size: 236 1108
BtgI C'CryG_G
Cuts at: 0 115 917 1344
Size: 115 802 427
Fragments arranged by size:
802 427 115
BtsI GCAGTG_nn'
Cuts at: 0 40 152 1344
Size: 40 112 1192
Fragments arranged by size:
1192 112 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 372 407 1344
Size: 372 35 937
Fragments arranged by size:
937 372 35
DraIII CAC_nnn'GTG
Cuts at: 0 171 1344
Size: 171 1173
EaeI y'GGCC_r
Cuts at: 0 644 761 821 1202 1308 1344
Size: 644 117 60 381 106 36
Fragments arranged by size:
644 381 117 106 60 36
EarI CTCTTCn'nnn_
Cuts at: 0 733 1018 1344
Size: 733 285 326
Fragments arranged by size:
733 326 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1170 1213 1344
Size: 1170 43 131
Fragments arranged by size:
1170 131 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 580 714 969 1153 1344
Size: 249 331 134 255 184 191
Fragments arranged by size:
331 255 249 191 184 134
EcoO109I rG'GnC_Cy
Cuts at: 0 70 122 375 669 887 1254 1344
Size: 70 52 253 294 218 367 90
Fragments arranged by size:
367 294 253 218 90 70 52
HaeII r_GCGC'y
Cuts at: 0 301 458 1273 1344
Size: 301 157 815 71
Fragments arranged by size:
815 301 157 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1344
Size: 1231 32 81
Fragments arranged by size:
1231 81 32
HincII GTy'rAC
Cuts at: 0 519 1344
Size: 519 825
KasI G'GCGC_C
Cuts at: 0 297 1344
Size: 297 1047
MscI TGG'CCA
Cuts at: 0 646 763 1204 1310 1344
Size: 646 117 441 106 34
Fragments arranged by size:
646 441 117 106 34
MslI CAynn'nnrTG
Cuts at: 0 256 337 769 916 1344
Size: 256 81 432 147 428
Fragments arranged by size:
432 428 256 147 81
MspA1I CmG'CkG
Cuts at: 0 960 1152 1344
Size: 960 192 192
Fragments arranged by size:
960 192 192
NarI GG'CG_CC
Cuts at: 0 298 1344
Size: 298 1046
NcoI C'CATG_G
Cuts at: 0 115 1344
Size: 115 1229
NspI r_CATG'y
Cuts at: 0 84 1344
Size: 84 1260
PflMI CCAn_nnn'nTGG
Cuts at: 0 181 615 1344
Size: 181 434 729
Fragments arranged by size:
729 434 181
PmlI CAC'GTG
Cuts at: 0 864 1127 1344
Size: 864 263 217
Fragments arranged by size:
864 263 217
PpuMI rG'GwC_Cy
Cuts at: 0 70 887 1344
Size: 70 817 457
Fragments arranged by size:
817 457 70
PspOMI G'GGCC_C
Cuts at: 0 122 1344
Size: 122 1222
PspXI vC'TCGA_Gb
Cuts at: 0 550 1344
Size: 550 794
PstI C_TGCA'G
Cuts at: 0 971 1214 1344
Size: 971 243 130
Fragments arranged by size:
971 243 130
PvuII CAG'CTG
Cuts at: 0 960 1344
Size: 960 384
SanDI GG'GwC_CC
Cuts at: 0 887 1344
Size: 887 457
SfcI C'TryA_G
Cuts at: 0 828 967 1210 1320 1344
Size: 828 139 243 110 24
Fragments arranged by size:
828 243 139 110 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 605 1344
Size: 605 739
SfoI GGC'GCC
Cuts at: 0 299 1344
Size: 299 1045
SmlI C'TyrA_G
Cuts at: 0 75 195 550 1344
Size: 75 120 355 794
Fragments arranged by size:
794 355 120 75
SphI G_CATG'C
Cuts at: 0 84 1344
Size: 84 1260
StyI C'CwwG_G
Cuts at: 0 115 126 647 665 1199 1298 1344
Size: 115 11 521 18 534 99 46
Fragments arranged by size:
534 521 115 99 46 18 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 524 1344
Size: 524 820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1071 1344
Size: 1071 273
XhoI C'TCGA_G
Cuts at: 0 550 1344
Size: 550 794
ZraI GAC'GTC
Cuts at: 0 753 1344
Size: 753 591
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AleI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BclI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EarI EciI Eco57MI
EcoO109I HaeII Hin4I HincII KasI MscI MslI MspA1I
NarI NcoI NspI PflMI PmlI PpuMI PspOMI PspXI
PstI PvuII SanDI SfcI SfiI SfoI SmlI SphI
StyI TaqII XcmI XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AgeI AhdI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BfrBI BglII BmgBI BmtI BplI BsaBI
BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrFI BsrGI
BssHII BstAPI BstBI BstZ17I BtgZI ClaI DraI DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MmeI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PshAI PsiI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XmaI XmnI