Restriction map for
pF1KE0451
(Linear) (Six-base) MAPSORT of: KIEE0451.seq Check: 33 from: 1 to: 924
KIEE0451.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 924
Size: 891 33
AleI CACnn'nnGTG
Cuts at: 0 203 274 924
Size: 203 71 650
Fragments arranged by size:
650 203 71
ApaI G_GGCC'C
Cuts at: 0 651 924
Size: 651 273
AvrII C'CTAG_G
Cuts at: 0 453 924
Size: 453 471
BanI G'GyrC_C
Cuts at: 0 21 924
Size: 21 903
BanII G_rGCy'C
Cuts at: 0 52 651 924
Size: 52 599 273
Fragments arranged by size:
599 273 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 363 924
Size: 363 561
BclI T'GATC_A
Cuts at: 0 485 924
Size: 485 439
Bme1580I G_kGCm'C
Cuts at: 0 210 651 924
Size: 210 441 273
Fragments arranged by size:
441 273 210
BmrI ACTGGGnnnn_n'
Cuts at: 0 624 924
Size: 624 300
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 163 533 889 924
Size: 163 370 356 35
Fragments arranged by size:
370 356 163 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 924
Size: 844 80
Bpu10I CC'TnA_GC
Cuts at: 0 362 924
Size: 362 562
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 530 560 924
Size: 530 30 364
Fragments arranged by size:
530 364 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 551 924
Size: 328 223 373
Fragments arranged by size:
373 328 223
BseYI C'CCAG_C
Cuts at: 0 123 531 592 924
Size: 123 408 61 332
Fragments arranged by size:
408 332 123 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 924
Size: 82 842
Bsp1286I G_dGCh'C
Cuts at: 0 52 210 651 924
Size: 52 158 441 273
Fragments arranged by size:
441 273 158 52
BsrBI CCG'CTC
Cuts at: 0 351 924
Size: 351 573
BsrFI r'CCGG_y
Cuts at: 0 554 924
Size: 554 370
BsrGI T'GTAC_A
Cuts at: 0 609 924
Size: 609 315
BstEII G'GTnAC_C
Cuts at: 0 745 924
Size: 745 179
BtsI GCAGTG_nn'
Cuts at: 0 315 924
Size: 315 609
EaeI y'GGCC_r
Cuts at: 0 559 924
Size: 559 365
EarI CTCTTCn'nnn_
Cuts at: 0 563 924
Size: 563 361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 891 924
Size: 844 47 33
Fragments arranged by size:
844 47 33
EcoO109I rG'GnC_Cy
Cuts at: 0 647 924
Size: 647 277
EcoRV GAT'ATC
Cuts at: 0 226 924
Size: 226 698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 387 924
Size: 387 537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 215 247 825 857 924
Size: 215 32 578 32 67
Fragments arranged by size:
578 215 67 32 32
HincII GTy'rAC
Cuts at: 0 156 924
Size: 156 768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 924
Size: 672 252
MslI CAynn'nnrTG
Cuts at: 0 203 233 274 916 924
Size: 203 30 41 642 8
Fragments arranged by size:
642 203 41 30 8
PasI CC'CwG_GG
Cuts at: 0 79 210 924
Size: 79 131 714
Fragments arranged by size:
714 131 79
PspOMI G'GGCC_C
Cuts at: 0 647 924
Size: 647 277
PstI C_TGCA'G
Cuts at: 0 509 924
Size: 509 415
ScaI AGT'ACT
Cuts at: 0 601 924
Size: 601 323
SfcI C'TryA_G
Cuts at: 0 505 924
Size: 505 419
SgrAI Cr'CCGG_yG
Cuts at: 0 554 924
Size: 554 370
StyI C'CwwG_G
Cuts at: 0 453 861 924
Size: 453 408 63
Fragments arranged by size:
453 408 63
TatI w'GTAC_w
Cuts at: 0 599 609 924
Size: 599 10 315
Fragments arranged by size:
599 315 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 924
Size: 585 339
XmnI GAAnn'nnTTC
Cuts at: 0 683 924
Size: 683 241
Enzymes that do cut:
AcuI AleI ApaI AvrII BanI BanII BbsI BclI
Bme1580I BmrI BplI BpmI Bpu10I BsaXI BseRI BseYI
BsgI Bsp1286I BsrBI BsrFI BsrGI BstEII BtsI EaeI
EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII MmeI
MslI PasI PspOMI PstI ScaI SfcI SgrAI StyI
TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
ZraI