Restriction map for
pF1KE0452
(Linear) (Six-base) MAPSORT of: KIEE0452.seq Check: 1389 from: 1 to: 1386
KIEE0452.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
Acc65I G'GTAC_C
Cuts at: 0 563 612 1386
Size: 563 49 774
Fragments arranged by size:
774 563 49
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1386
Size: 1153 233
AflIII A'CryG_T
Cuts at: 0 309 698 1386
Size: 309 389 688
Fragments arranged by size:
688 389 309
ApaI G_GGCC'C
Cuts at: 0 1072 1386
Size: 1072 314
ApoI r'AATT_y
Cuts at: 0 171 222 956 1386
Size: 171 51 734 430
Fragments arranged by size:
734 430 171 51
AvaI C'yCGr_G
Cuts at: 0 488 1386
Size: 488 898
BamHI G'GATC_C
Cuts at: 0 492 1386
Size: 492 894
BanI G'GyrC_C
Cuts at: 0 563 612 1386
Size: 563 49 774
Fragments arranged by size:
774 563 49
BanII G_rGCy'C
Cuts at: 0 114 1072 1386
Size: 114 958 314
Fragments arranged by size:
958 314 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 775 1386
Size: 775 611
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1099 1133 1386
Size: 1099 34 253
Fragments arranged by size:
1099 253 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1069 1386
Size: 1069 317
BclI T'GATC_A
Cuts at: 0 690 1386
Size: 690 696
BfrBI ATG'CAT
Cuts at: 0 1360 1386
Size: 1360 26
BlpI GC'TnA_GC
Cuts at: 0 531 1386
Size: 531 855
Bme1580I G_kGCm'C
Cuts at: 0 1072 1386
Size: 1072 314
BmtI G_CTAG'C
Cuts at: 0 1019 1386
Size: 1019 367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 438 1386
Size: 422 16 948
Fragments arranged by size:
948 422 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 81 863 1386
Size: 81 782 523
Fragments arranged by size:
782 523 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 1386
Size: 436 30 920
Fragments arranged by size:
920 436 30
BseYI C'CCAG_C
Cuts at: 0 1180 1386
Size: 1180 206
BsiHKAI G_wGCw'C
Cuts at: 0 114 1386
Size: 114 1272
Bsp1286I G_dGCh'C
Cuts at: 0 114 1072 1386
Size: 114 958 314
Fragments arranged by size:
958 314 114
BsrDI GCAATG_nn'
Cuts at: 0 783 1386
Size: 783 603
BsrGI T'GTAC_A
Cuts at: 0 701 1386
Size: 701 685
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1357 1386
Size: 1357 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 1386
Size: 624 762
BstYI r'GATC_y
Cuts at: 0 492 1386
Size: 492 894
Bsu36I CC'TnA_GG
Cuts at: 0 852 1386
Size: 852 534
BtgI C'CryG_G
Cuts at: 0 1099 1386
Size: 1099 287
DraI TTT'AAA
Cuts at: 0 77 227 1386
Size: 77 150 1159
Fragments arranged by size:
1159 150 77
EarI CTCTTCn'nnn_
Cuts at: 0 788 1386
Size: 788 598
EcoICRI GAG'CTC
Cuts at: 0 112 1386
Size: 112 1274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 438 1153 1386
Size: 422 16 715 233
Fragments arranged by size:
715 422 233 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 753 1386
Size: 753 633
EcoO109I rG'GnC_Cy
Cuts at: 0 393 1386
Size: 393 993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1370 1386
Size: 1370 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 1386
Size: 436 32 918
Fragments arranged by size:
918 436 32
HincII GTy'rAC
Cuts at: 0 845 1386
Size: 845 541
HpaI GTT'AAC
Cuts at: 0 845 1386
Size: 845 541
KpnI G_GTAC'C
Cuts at: 0 567 616 1386
Size: 567 49 770
Fragments arranged by size:
770 567 49
MfeI C'AATT_G
Cuts at: 0 539 1386
Size: 539 847
MslI CAynn'nnrTG
Cuts at: 0 926 1386
Size: 926 460
MspA1I CmG'CkG
Cuts at: 0 1374 1386
Size: 1374 12
NcoI C'CATG_G
Cuts at: 0 1099 1386
Size: 1099 287
NheI G'CTAG_C
Cuts at: 0 1015 1386
Size: 1015 371
NsiI A_TGCA'T
Cuts at: 0 1362 1386
Size: 1362 24
NspI r_CATG'y
Cuts at: 0 313 702 1386
Size: 313 389 684
Fragments arranged by size:
684 389 313
PciI A'CATG_T
Cuts at: 0 309 698 1386
Size: 309 389 688
Fragments arranged by size:
688 389 309
PpuMI rG'GwC_Cy
Cuts at: 0 393 1386
Size: 393 993
PspOMI G'GGCC_C
Cuts at: 0 1068 1386
Size: 1068 318
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1245 1277 1386
Size: 1245 32 109
Fragments arranged by size:
1245 109 32
PvuII CAG'CTG
Cuts at: 0 1374 1386
Size: 1374 12
SacI G_AGCT'C
Cuts at: 0 114 1386
Size: 114 1272
SanDI GG'GwC_CC
Cuts at: 0 393 1386
Size: 393 993
SapI GCTCTTCn'nnn_
Cuts at: 0 788 1386
Size: 788 598
SexAI A'CCwGG_T
Cuts at: 0 1293 1386
Size: 1293 93
SspI AAT'ATT
Cuts at: 0 1052 1386
Size: 1052 334
StyI C'CwwG_G
Cuts at: 0 1099 1386
Size: 1099 287
TatI w'GTAC_w
Cuts at: 0 546 701 1386
Size: 546 155 685
Fragments arranged by size:
685 546 155
Enzymes that do cut:
Acc65I AcuI AflIII ApaI ApoI AvaI BamHI BanI
BanII BbsI BcgI BciVI BclI BfrBI BlpI Bme1580I
BmtI BpmI BsaI BsaXI BseYI BsiHKAI Bsp1286I BsrDI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI DraI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HpaI
KpnI MfeI MslI MspA1I NcoI NheI NsiI NspI
PciI PpuMI PspOMI PsrI PvuII SacI SanDI SapI
SexAI SspI StyI TatI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BglI BglII BmgBI BmrI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoRI EcoRV FseI FspI FspAI HaeII HindIII KasI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI RsrII SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI