Restriction map for
pF1KE0454
(Linear) (Six-base) MAPSORT of: KIEE0454.seq Check: 8372 from: 1 to: 882
KIEE0454.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 882
Size: 386 496
AflII C'TTAA_G
Cuts at: 0 645 882
Size: 645 237
AflIII A'CryG_T
Cuts at: 0 611 882
Size: 611 271
AgeI A'CCGG_T
Cuts at: 0 122 882
Size: 122 760
ApaI G_GGCC'C
Cuts at: 0 31 882
Size: 31 851
ApoI r'AATT_y
Cuts at: 0 527 595 658 805 882
Size: 527 68 63 147 77
Fragments arranged by size:
527 147 77 68 63
AvaI C'yCGr_G
Cuts at: 0 409 882
Size: 409 473
BamHI G'GATC_C
Cuts at: 0 147 882
Size: 147 735
BanII G_rGCy'C
Cuts at: 0 31 882
Size: 31 851
BbsI GAAGACnn'nnnn_
Cuts at: 0 803 830 882
Size: 803 27 52
Fragments arranged by size:
803 52 27
BglI GCCn_nnn'nGGC
Cuts at: 0 35 882
Size: 35 847
Bme1580I G_kGCm'C
Cuts at: 0 31 882
Size: 31 851
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 858 882
Size: 858 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 882
Size: 322 560
BsaWI w'CCGG_w
Cuts at: 0 122 882
Size: 122 760
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 245 275 882
Size: 245 30 607
Fragments arranged by size:
607 245 30
BsmI GAATG_Cn'
Cuts at: 0 874 882
Size: 874 8
Bsp1286I G_dGCh'C
Cuts at: 0 31 882
Size: 31 851
BspHI T'CATG_A
Cuts at: 0 545 882
Size: 545 337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 882
Size: 386 496
BsrFI r'CCGG_y
Cuts at: 0 122 882
Size: 122 760
BsrGI T'GTAC_A
Cuts at: 0 368 882
Size: 368 514
BstYI r'GATC_y
Cuts at: 0 53 147 558 603 882
Size: 53 94 411 45 279
Fragments arranged by size:
411 279 94 53 45
BtgI C'CryG_G
Cuts at: 0 32 95 882
Size: 32 63 787
Fragments arranged by size:
787 63 32
BtsI GCAGTG_nn'
Cuts at: 0 421 882
Size: 421 461
DraI TTT'AAA
Cuts at: 0 810 882
Size: 810 72
EaeI y'GGCC_r
Cuts at: 0 315 882
Size: 315 567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 882
Size: 322 560
EcoO109I rG'GnC_Cy
Cuts at: 0 28 882
Size: 28 854
FspI TGC'GCA
Cuts at: 0 14 882
Size: 14 868
HincII GTy'rAC
Cuts at: 0 540 882
Size: 540 342
MscI TGG'CCA
Cuts at: 0 317 882
Size: 317 565
MspA1I CmG'CkG
Cuts at: 0 18 34 41 396 882
Size: 18 16 7 355 486
Fragments arranged by size:
486 355 18 16 7
NcoI C'CATG_G
Cuts at: 0 95 882
Size: 95 787
NspI r_CATG'y
Cuts at: 0 615 882
Size: 615 267
PciI A'CATG_T
Cuts at: 0 611 882
Size: 611 271
PfoI T'CCnGG_A
Cuts at: 0 191 882
Size: 191 691
PspOMI G'GGCC_C
Cuts at: 0 27 882
Size: 27 855
PspXI vC'TCGA_Gb
Cuts at: 0 409 882
Size: 409 473
PvuII CAG'CTG
Cuts at: 0 18 396 882
Size: 18 378 486
Fragments arranged by size:
486 378 18
SacII CC_GC'GG
Cuts at: 0 35 882
Size: 35 847
ScaI AGT'ACT
Cuts at: 0 277 882
Size: 277 605
SfcI C'TryA_G
Cuts at: 0 702 882
Size: 702 180
SmlI C'TyrA_G
Cuts at: 0 409 645 882
Size: 409 236 237
Fragments arranged by size:
409 237 236
SspI AAT'ATT
Cuts at: 0 859 882
Size: 859 23
StyI C'CwwG_G
Cuts at: 0 95 882
Size: 95 787
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 232 882
Size: 232 650
TatI w'GTAC_w
Cuts at: 0 275 368 882
Size: 275 93 514
Fragments arranged by size:
514 275 93
XbaI T'CTAG_A
Cuts at: 0 522 882
Size: 522 360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 882
Size: 255 627
XhoI C'TCGA_G
Cuts at: 0 409 882
Size: 409 473
XmnI GAAnn'nnTTC
Cuts at: 0 860 882
Size: 860 22
Enzymes that do cut:
AarI AflII AflIII AgeI ApaI ApoI AvaI BamHI
BanII BbsI BglI Bme1580I BplI BpmI BsaWI BsaXI
BsmI Bsp1286I BspHI BspMI BsrFI BsrGI BstYI BtgI
BtsI DraI EaeI Eco57MI EcoO109I FspI HincII MscI
MspA1I NcoI NspI PciI PfoI PspOMI PspXI PvuII
SacII ScaI SfcI SmlI SspI StyI TaqII TatI
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HaeII Hin4I HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI RsrII SacI SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XmaI ZraI