(Linear) (Six-base) MAPSORT of: KIEE0455.seq  Check: 427  from: 1  to: 678



KIEE0455.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AflIII A'CryG_T

Cuts at:      0     212     344     668     678 
   Size:        212     132     324      10

  Fragments arranged by size: 

                 324     212     132      10
 
AleI CACnn'nnGTG

Cuts at:      0     601     678 
   Size:        601      77
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      94     678 
   Size:         94     584
 
AlwNI CAG_nnn'CTG

Cuts at:      0      44     678 
   Size:         44     634
 
BanI G'GyrC_C

Cuts at:      0      70     678 
   Size:         70     608
 
BclI T'GATC_A

Cuts at:      0      98     678 
   Size:         98     580
 
BglII A'GATC_T

Cuts at:      0     323     678 
   Size:        323     355
 
Bme1580I G_kGCm'C

Cuts at:      0     138     678 
   Size:        138     540
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     187     678 
   Size:        187     491
 
BsaAI yAC'GTr

Cuts at:      0     669     678 
   Size:        669       9
 
BsaBI GATnn'nnATC

Cuts at:      0     149     678 
   Size:        149     529
 
BsaHI Gr'CG_yC

Cuts at:      0      86     678 
   Size:         86     592
 
BsaWI w'CCGG_w

Cuts at:      0     147     678 
   Size:        147     531
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     552     678 
   Size:        552     126
 
Bsp1286I G_dGCh'C

Cuts at:      0     138     678 
   Size:        138     540
 
BspEI T'CCGG_A

Cuts at:      0     147     678 
   Size:        147     531
 
BspHI T'CATG_A

Cuts at:      0     242     678 
   Size:        242     436
 
BsrDI GCAATG_nn'

Cuts at:      0     218     224     597     678 
   Size:        218       6     373      81

  Fragments arranged by size: 

                 373     218      81       6
 
BsrFI r'CCGG_y

Cuts at:      0      67     678 
   Size:         67     611
 
BstYI r'GATC_y

Cuts at:      0     323     678 
   Size:        323     355
 
EarI CTCTTCn'nnn_

Cuts at:      0     242     678 
   Size:        242     436
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     528     678 
   Size:        528     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     419     424     451     456     678 
   Size:        419       5      27       5     222

  Fragments arranged by size: 

                 419     222      27       5       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     678 
   Size:         59     619
 
MslI CAynn'nnrTG

Cuts at:      0     442     601     678 
   Size:        442     159      77

  Fragments arranged by size: 

                 442     159      77
 
MspA1I CmG'CkG

Cuts at:      0      41     678 
   Size:         41     637
 
NspI r_CATG'y

Cuts at:      0     216     348     678 
   Size:        216     132     330

  Fragments arranged by size: 

                 330     216     132
 
PciI A'CATG_T

Cuts at:      0     212     344     678 
   Size:        212     132     334

  Fragments arranged by size: 

                 334     212     132
 
PvuII CAG'CTG

Cuts at:      0      41     678 
   Size:         41     637
 
SfcI C'TryA_G

Cuts at:      0     423     678 
   Size:        423     255
 
StyI C'CwwG_G

Cuts at:      0     647     678 
   Size:        647      31
 

 Enzymes that do cut:

   AflIII     AleI     AlfI    AlwNI     BanI     BclI    BglII Bme1580I
     BmrI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI Bsp1286I    BspEI
    BspHI    BsrDI    BsrFI    BstYI     EarI     FalI    Hin4I     MmeI
     MslI   MspA1I     NspI     PciI    PvuII     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI