(Linear) (Six-base) MAPSORT of: KIEE0459.seq  Check: 674  from: 1  to: 474



KIEE0459.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      76     474 
   Size:         76     398
 
BanII G_rGCy'C

Cuts at:      0     113     474 
   Size:        113     361
 
BfrBI ATG'CAT

Cuts at:      0     345     474 
   Size:        345     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     306     474 
   Size:        306     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     396     474 
   Size:         80     316      78

  Fragments arranged by size: 

                 316      80      78
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     309     474 
   Size:        309     165
 
BseYI C'CCAG_C

Cuts at:      0     133     474 
   Size:        133     341
 
BsmI GAATG_Cn'

Cuts at:      0     241     474 
   Size:        241     233
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     474 
   Size:        113     361
 
BspHI T'CATG_A

Cuts at:      0      48     474 
   Size:         48     426
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107     474 
   Size:        107     367
 
BsrFI r'CCGG_y

Cuts at:      0      11     437     474 
   Size:         11     426      37

  Fragments arranged by size: 

                 426      37      11
 
BssHII G'CGCG_C

Cuts at:      0     137     474 
   Size:        137     337
 
BstYI r'GATC_y

Cuts at:      0     370     474 
   Size:        370     104
 
Bsu36I CC'TnA_GG

Cuts at:      0      69     474 
   Size:         69     405
 
BtgI C'CryG_G

Cuts at:      0      28     474 
   Size:         28     446
 
BtsI GCAGTG_nn'

Cuts at:      0     286     474 
   Size:        286     188
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     383     474 
   Size:        383      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     396     474 
   Size:         80     316      78

  Fragments arranged by size: 

                 316      80      78
 
EcoO109I rG'GnC_Cy

Cuts at:      0     300     416     474 
   Size:        300     116      58

  Fragments arranged by size: 

                 300     116      58
 
HaeII r_GCGC'y

Cuts at:      0      77     474 
   Size:         77     397
 
MslI CAynn'nnrTG

Cuts at:      0      47     474 
   Size:         47     427
 
MspA1I CmG'CkG

Cuts at:      0      30     474 
   Size:         30     444
 
NsiI A_TGCA'T

Cuts at:      0     347     474 
   Size:        347     127
 
NspI r_CATG'y

Cuts at:      0     349     474 
   Size:        349     125
 
PasI CC'CwG_GG

Cuts at:      0     218     474 
   Size:        218     256
 
PpuMI rG'GwC_Cy

Cuts at:      0     300     474 
   Size:        300     174
 
PstI C_TGCA'G

Cuts at:      0     118     449     474 
   Size:        118     331      25

  Fragments arranged by size: 

                 331     118      25
 
SacII CC_GC'GG

Cuts at:      0      31     474 
   Size:         31     443
 
SanDI GG'GwC_CC

Cuts at:      0     300     474 
   Size:        300     174
 
SfcI C'TryA_G

Cuts at:      0     114     445     474 
   Size:        114     331      29

  Fragments arranged by size: 

                 331     114      29
 
SphI G_CATG'C

Cuts at:      0     349     474 
   Size:        349     125
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     147     474 
   Size:        147     327
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     362     394     474 
   Size:        362      32      80

  Fragments arranged by size: 

                 362      80      32
 

 Enzymes that do cut:

    AlwNI    BanII    BfrBI     BmrI     BpmI     BsaI    BseYI     BsmI
 Bsp1286I    BspHI    BspMI    BsrFI   BssHII    BstYI   Bsu36I     BtgI
     BtsI     EciI  Eco57MI EcoO109I    HaeII     MslI   MspA1I     NsiI
     NspI     PasI    PpuMI     PstI    SacII    SanDI     SfcI     SphI
    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI