(Linear) (Six-base) MAPSORT of: KIEE0463.seq  Check: 5383  from: 1  to: 1248



KIEE0463.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     821    1219    1248 
   Size:        587     234     398      29

  Fragments arranged by size: 

                 587     398     234      29
 
AflIII A'CryG_T

Cuts at:      0     633    1248 
   Size:        633     615
 
AleI CACnn'nnGTG

Cuts at:      0    1066    1248 
   Size:       1066     182
 
AlwNI CAG_nnn'CTG

Cuts at:      0     447     964    1248 
   Size:        447     517     284

  Fragments arranged by size: 

                 517     447     284
 
ApoI r'AATT_y

Cuts at:      0    1159    1248 
   Size:       1159      89
 
AvaI C'yCGr_G

Cuts at:      0    1094    1248 
   Size:       1094     154
 
BanI G'GyrC_C

Cuts at:      0    1108    1248 
   Size:       1108     140
 
BanII G_rGCy'C

Cuts at:      0      19     335    1248 
   Size:         19     316     913

  Fragments arranged by size: 

                 913     316      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     507    1248 
   Size:        507     741
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1180    1248 
   Size:       1180      68
 
BglII A'GATC_T

Cuts at:      0     519    1248 
   Size:        519     729
 
BmgBI CAC'GTC

Cuts at:      0     721    1248 
   Size:        721     527
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     242    1248 
   Size:        242    1006
 
Bpu10I CC'TnA_GC

Cuts at:      0     839    1248 
   Size:        839     409
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33    1248 
   Size:         33    1215
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1044    1248 
   Size:       1044     204
 
BsaAI yAC'GTr

Cuts at:      0     636    1248 
   Size:        636     612
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     569     599    1248 
   Size:        569      30     649

  Fragments arranged by size: 

                 649     569      30
 
BseYI C'CCAG_C

Cuts at:      0    1101    1248 
   Size:       1101     147
 
BsiEI CG_ry'CG

Cuts at:      0    1116    1248 
   Size:       1116     132
 
BsiHKAI G_wGCw'C

Cuts at:      0      19     335    1248 
   Size:         19     316     913

  Fragments arranged by size: 

                 913     316      19
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     335    1248 
   Size:         19     316     913

  Fragments arranged by size: 

                 913     316      19
 
BsrBI CCG'CTC

Cuts at:      0    1220    1248 
   Size:       1220      28
 
BsrFI r'CCGG_y

Cuts at:      0    1203    1248 
   Size:       1203      45
 
BssSI C'ACGA_G

Cuts at:      0    1120    1248 
   Size:       1120     128
 
BstYI r'GATC_y

Cuts at:      0     519    1248 
   Size:        519     729
 
BtsI GCAGTG_nn'

Cuts at:      0     496    1248 
   Size:        496     752
 
DraIII CAC_nnn'GTG

Cuts at:      0     975    1248 
   Size:        975     273
 
EarI CTCTTCn'nnn_

Cuts at:      0    1132    1235    1248 
   Size:       1132     103      13

  Fragments arranged by size: 

                1132     103      13
 
EcoICRI GAG'CTC

Cuts at:      0      17     333    1248 
   Size:         17     316     915

  Fragments arranged by size: 

                 915     316      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     821    1219    1248 
   Size:        587     234     398      29

  Fragments arranged by size: 

                 587     398     234      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     340    1248 
   Size:        340     908
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1098    1248 
   Size:       1098     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      97     129     296     328    1248 
   Size:         97      32     167      32     920

  Fragments arranged by size: 

                 920     167      97      32      32
 
MfeI C'AATT_G

Cuts at:      0     772    1248 
   Size:        772     476
 
MslI CAynn'nnrTG

Cuts at:      0     467     878    1066    1248 
   Size:        467     411     188     182

  Fragments arranged by size: 

                 467     411     188     182
 
NaeI GCC'GGC

Cuts at:      0    1205    1248 
   Size:       1205      43
 
NgoMIV G'CCGG_C

Cuts at:      0    1203    1248 
   Size:       1203      45
 
NspI r_CATG'y

Cuts at:      0     811    1248 
   Size:        811     437
 
PfoI T'CCnGG_A

Cuts at:      0     335    1132    1248 
   Size:        335     797     116

  Fragments arranged by size: 

                 797     335     116
 
PpuMI rG'GwC_Cy

Cuts at:      0    1098    1248 
   Size:       1098     150
 
PsiI TTA'TAA

Cuts at:      0     511    1248 
   Size:        511     737
 
SacI G_AGCT'C

Cuts at:      0      19     335    1248 
   Size:         19     316     913

  Fragments arranged by size: 

                 913     316      19
 
SanDI GG'GwC_CC

Cuts at:      0    1098    1248 
   Size:       1098     150
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1132    1235    1248 
   Size:       1132     103      13

  Fragments arranged by size: 

                1132     103      13
 
ScaI AGT'ACT

Cuts at:      0     587    1248 
   Size:        587     661
 
SmaI CCC'GGG

Cuts at:      0    1096    1248 
   Size:       1096     152
 
SmlI C'TyrA_G

Cuts at:      0      12    1248 
   Size:         12    1236
 
TatI w'GTAC_w

Cuts at:      0     585    1248 
   Size:        585     663
 
XmaI C'CCGG_G

Cuts at:      0    1094    1248 
   Size:       1094     154
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApoI     AvaI     BanI    BanII
     BbsI     BglI    BglII    BmgBI     BmrI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BsrBI    BsrFI
    BssSI    BstYI     BtsI   DraIII     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    Hin4I     MfeI     MslI     NaeI   NgoMIV     NspI     PfoI
    PpuMI     PsiI     SacI    SanDI     SapI     ScaI     SmaI     SmlI
     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BlpI Bme1580I     BmtI     BplI     BpmI    BsaBI    BsaHI
    BsaWI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI     SbfI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI