(Linear) (Six-base) MAPSORT of: KIEE0467.seq  Check: 5399  from: 1  to: 651



KIEE0467.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487     616     651 
   Size:        487     129      35

  Fragments arranged by size: 

                 487     129      35
 
ApaI G_GGCC'C

Cuts at:      0      57     651 
   Size:         57     594
 
ApoI r'AATT_y

Cuts at:      0     517     651 
   Size:        517     134
 
AvaI C'yCGr_G

Cuts at:      0      48     385     651 
   Size:         48     337     266

  Fragments arranged by size: 

                 337     266      48
 
BanI G'GyrC_C

Cuts at:      0     397     651 
   Size:        397     254
 
BanII G_rGCy'C

Cuts at:      0      57     651 
   Size:         57     594
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     547     581     651 
   Size:        547      34      70

  Fragments arranged by size: 

                 547      70      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      16     651 
   Size:         16     635
 
Bme1580I G_kGCm'C

Cuts at:      0      57     651 
   Size:         57     594
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     373     651 
   Size:        373     278
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     563     651 
   Size:        563      88
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556     651 
   Size:        556      95
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     487     651 
   Size:        487     164
 
BseYI C'CCAG_C

Cuts at:      0     351     651 
   Size:        351     300
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     393     651 
   Size:        393     258
 
BsiHKAI G_wGCw'C

Cuts at:      0     542     651 
   Size:        542     109
 
Bsp1286I G_dGCh'C

Cuts at:      0      57     542     651 
   Size:         57     485     109

  Fragments arranged by size: 

                 485     109      57
 
BtgI C'CryG_G

Cuts at:      0     401     413     651 
   Size:        401      12     238

  Fragments arranged by size: 

                 401     238      12
 
EaeI y'GGCC_r

Cuts at:      0     319     651 
   Size:        319     332
 
EarI CTCTTCn'nnn_

Cuts at:      0     419     524     592     651 
   Size:        419     105      68      59

  Fragments arranged by size: 

                 419     105      68      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487     563     616     651 
   Size:        487      76      53      35

  Fragments arranged by size: 

                 487      76      53      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0      53     477     651 
   Size:         53     424     174

  Fragments arranged by size: 

                 424     174      53
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     651 
   Size:        205     446
 
MspA1I CmG'CkG

Cuts at:      0     142     507     651 
   Size:        142     365     144

  Fragments arranged by size: 

                 365     144     142
 
NcoI C'CATG_G

Cuts at:      0     401     651 
   Size:        401     250
 
PpuMI rG'GwC_Cy

Cuts at:      0     477     651 
   Size:        477     174
 
PshAI GACnn'nnGTC

Cuts at:      0     232     651 
   Size:        232     419
 
PspOMI G'GGCC_C

Cuts at:      0      53     651 
   Size:         53     598
 
PvuII CAG'CTG

Cuts at:      0     142     507     651 
   Size:        142     365     144

  Fragments arranged by size: 

                 365     144     142
 
SapI GCTCTTCn'nnn_

Cuts at:      0     524     592     651 
   Size:        524      68      59

  Fragments arranged by size: 

                 524      68      59
 
SmaI CCC'GGG

Cuts at:      0      50     651 
   Size:         50     601
 
SmlI C'TyrA_G

Cuts at:      0     535     651 
   Size:        535     116
 
StuI AGG'CCT

Cuts at:      0     554     651 
   Size:        554      97
 
StyI C'CwwG_G

Cuts at:      0     401     651 
   Size:        401     250
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     381     413     608     640     651 
   Size:        381      32     195      32      11

  Fragments arranged by size: 

                 381     195      32      32      11
 
XmaI C'CCGG_G

Cuts at:      0      48     651 
   Size:         48     603
 
XmnI GAAnn'nnTTC

Cuts at:      0     286     500     651 
   Size:        286     214     151

  Fragments arranged by size: 

                 286     214     151
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI     AvaI     BanI    BanII     BcgI     BglI
 Bme1580I     BmrI     BpmI    BpuEI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I     BtgI     EaeI     EarI  Eco57MI EcoO109I     MmeI   MspA1I
     NcoI    PpuMI    PshAI   PspOMI    PvuII     SapI     SmaI     SmlI
     StuI     StyI     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     ZraI