Restriction map for
pF1KE0469
(Linear) (Six-base) MAPSORT of: KIEE0469.seq Check: 1530 from: 1 to: 624
KIEE0469.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 474 624
Size: 474 150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 624
Size: 221 403
AhdI GACnn_n'nnGTC
Cuts at: 0 67 624
Size: 67 557
AlwNI CAG_nnn'CTG
Cuts at: 0 489 624
Size: 489 135
ApaI G_GGCC'C
Cuts at: 0 9 18 145 624
Size: 9 9 127 479
Fragments arranged by size:
479 127 9 9
BanI G'GyrC_C
Cuts at: 0 45 624
Size: 45 579
BanII G_rGCy'C
Cuts at: 0 9 18 85 145 624
Size: 9 9 67 60 479
Fragments arranged by size:
479 67 60 9 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 238 624
Size: 209 29 386
Fragments arranged by size:
386 209 29
BglI GCCn_nnn'nGGC
Cuts at: 0 13 624
Size: 13 611
BglII A'GATC_T
Cuts at: 0 452 624
Size: 452 172
Bme1580I G_kGCm'C
Cuts at: 0 9 18 50 145 624
Size: 9 9 32 95 479
Fragments arranged by size:
479 95 32 9 9
BmgBI CAC'GTC
Cuts at: 0 294 624
Size: 294 330
BmrI ACTGGGnnnn_n'
Cuts at: 0 61 310 624
Size: 61 249 314
Fragments arranged by size:
314 249 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 624
Size: 194 430
BsaHI Gr'CG_yC
Cuts at: 0 63 624
Size: 63 561
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 191 447 577 624
Size: 191 256 130 47
Fragments arranged by size:
256 191 130 47
BseYI C'CCAG_C
Cuts at: 0 469 624
Size: 469 155
Bsp1286I G_dGCh'C
Cuts at: 0 9 18 50 85 145 624
Size: 9 9 32 35 60 479
Fragments arranged by size:
479 60 35 32 9 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 624
Size: 474 150
BsrFI r'CCGG_y
Cuts at: 0 95 624
Size: 95 529
BsrGI T'GTAC_A
Cuts at: 0 404 624
Size: 404 220
BstYI r'GATC_y
Cuts at: 0 452 624
Size: 452 172
Bsu36I CC'TnA_GG
Cuts at: 0 309 624
Size: 309 315
BtgI C'CryG_G
Cuts at: 0 614 624
Size: 614 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 49 624
Size: 49 575
EaeI y'GGCC_r
Cuts at: 0 97 447 504 624
Size: 97 350 57 120
Fragments arranged by size:
350 120 97 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 221 624
Size: 194 27 403
Fragments arranged by size:
403 194 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 176 624
Size: 176 448
EcoO109I rG'GnC_Cy
Cuts at: 0 5 14 141 142 624
Size: 5 9 127 1 482
Fragments arranged by size:
482 127 9 5 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 624
Size: 53 32 539
Fragments arranged by size:
539 53 32
HindIII A'AGCT_T
Cuts at: 0 380 439 624
Size: 380 59 185
Fragments arranged by size:
380 185 59
MscI TGG'CCA
Cuts at: 0 449 506 624
Size: 449 57 118
Fragments arranged by size:
449 118 57
PasI CC'CwG_GG
Cuts at: 0 9 85 624
Size: 9 76 539
Fragments arranged by size:
539 76 9
PspOMI G'GGCC_C
Cuts at: 0 5 14 141 624
Size: 5 9 127 483
Fragments arranged by size:
483 127 9 5
PstI C_TGCA'G
Cuts at: 0 341 624
Size: 341 283
SfcI C'TryA_G
Cuts at: 0 239 337 624
Size: 239 98 287
Fragments arranged by size:
287 239 98
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 13 624
Size: 13 611
StyI C'CwwG_G
Cuts at: 0 519 624
Size: 519 105
TatI w'GTAC_w
Cuts at: 0 404 624
Size: 404 220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 274 624
Size: 274 350
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI BanI BanII BbsI
BglI BglII Bme1580I BmgBI BmrI BpmI BsaHI BseRI
BseYI Bsp1286I BspMI BsrFI BsrGI BstYI Bsu36I BtgI
BtgZI EaeI Eco57MI EcoNI EcoO109I Hin4I HindIII MscI
PasI PspOMI PstI SfcI SfiI StyI TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BlpI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI