Restriction map for
pF1KE0482
(Linear) (Six-base) MAPSORT of: KIEE0482.seq Check: 6974 from: 1 to: 993
KIEE0482.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 0 857 993
Size: 857 136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 187 557 803 993
Size: 47 140 370 246 190
Fragments arranged by size:
370 246 190 140 47
AlwNI CAG_nnn'CTG
Cuts at: 0 335 475 535 930 972 993
Size: 335 140 60 395 42 21
Fragments arranged by size:
395 335 140 60 42 21
ApaI G_GGCC'C
Cuts at: 0 702 993
Size: 702 291
AvaI C'yCGr_G
Cuts at: 0 776 993
Size: 776 217
BamHI G'GATC_C
Cuts at: 0 800 993
Size: 800 193
BanI G'GyrC_C
Cuts at: 0 411 769 993
Size: 411 358 224
Fragments arranged by size:
411 358 224
BanII G_rGCy'C
Cuts at: 0 702 798 953 993
Size: 702 96 155 40
Fragments arranged by size:
702 155 96 40
BbvCI CC'TCA_GC
Cuts at: 0 902 910 993
Size: 902 8 83
Fragments arranged by size:
902 83 8
BglII A'GATC_T
Cuts at: 0 959 993
Size: 959 34
Bme1580I G_kGCm'C
Cuts at: 0 303 702 993
Size: 303 399 291
Fragments arranged by size:
399 303 291
BmrI ACTGGGnnnn_n'
Cuts at: 0 58 494 993
Size: 58 436 499
Fragments arranged by size:
499 436 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 993
Size: 952 41
Bpu10I CC'TnA_GC
Cuts at: 0 902 910 923 993
Size: 902 8 13 70
Fragments arranged by size:
902 70 13 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 806 919 949 993
Size: 806 113 30 44
Fragments arranged by size:
806 113 44 30
BseYI C'CCAG_C
Cuts at: 0 451 462 993
Size: 451 11 531
Fragments arranged by size:
531 451 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 321 993
Size: 229 92 672
Fragments arranged by size:
672 229 92
BsiHKAI G_wGCw'C
Cuts at: 0 512 953 993
Size: 512 441 40
Fragments arranged by size:
512 441 40
Bsp1286I G_dGCh'C
Cuts at: 0 303 512 702 798 953 993
Size: 303 209 190 96 155 40
Fragments arranged by size:
303 209 190 155 96 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 475 892 993
Size: 81 394 417 101
Fragments arranged by size:
417 394 101 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 993
Size: 504 489
BstYI r'GATC_y
Cuts at: 0 272 800 959 993
Size: 272 528 159 34
Fragments arranged by size:
528 272 159 34
BstZ17I GTA'TAC
Cuts at: 0 858 993
Size: 858 135
BtsI GCAGTG_nn'
Cuts at: 0 788 993
Size: 788 205
EarI CTCTTCn'nnn_
Cuts at: 0 23 100 314 993
Size: 23 77 214 679
Fragments arranged by size:
679 214 77 23
EcoICRI GAG'CTC
Cuts at: 0 951 993
Size: 951 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 187 557 803 952 993
Size: 47 140 370 246 149 41
Fragments arranged by size:
370 246 149 140 47 41
EcoO109I rG'GnC_Cy
Cuts at: 0 698 993
Size: 698 295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 36 993
Size: 36 957
HaeII r_GCGC'y
Cuts at: 0 374 993
Size: 374 619
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 785 817 993
Size: 785 32 176
Fragments arranged by size:
785 176 32
HincII GTy'rAC
Cuts at: 0 849 993
Size: 849 144
HindIII A'AGCT_T
Cuts at: 0 172 292 993
Size: 172 120 701
Fragments arranged by size:
701 172 120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 993
Size: 371 622
MslI CAynn'nnrTG
Cuts at: 0 875 993
Size: 875 118
MspA1I CmG'CkG
Cuts at: 0 451 733 993
Size: 451 282 260
Fragments arranged by size:
451 282 260
NdeI CA'TA_TG
Cuts at: 0 183 993
Size: 183 810
PasI CC'CwG_GG
Cuts at: 0 134 993
Size: 134 859
PfoI T'CCnGG_A
Cuts at: 0 47 275 993
Size: 47 228 718
Fragments arranged by size:
718 228 47
PspOMI G'GGCC_C
Cuts at: 0 698 993
Size: 698 295
PstI C_TGCA'G
Cuts at: 0 450 993
Size: 450 543
PvuII CAG'CTG
Cuts at: 0 451 733 993
Size: 451 282 260
Fragments arranged by size:
451 282 260
SacI G_AGCT'C
Cuts at: 0 953 993
Size: 953 40
SfcI C'TryA_G
Cuts at: 0 446 993
Size: 446 547
SmaI CCC'GGG
Cuts at: 0 778 993
Size: 778 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 584 624 993
Size: 584 40 369
Fragments arranged by size:
584 369 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 792 824 993
Size: 792 32 169
Fragments arranged by size:
792 169 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 501 993
Size: 348 153 492
Fragments arranged by size:
492 348 153
XmaI C'CCGG_G
Cuts at: 0 776 993
Size: 776 217
XmnI GAAnn'nnTTC
Cuts at: 0 109 293 993
Size: 109 184 700
Fragments arranged by size:
700 184 109
Enzymes that do cut:
AccI AcuI AlwNI ApaI AvaI BamHI BanI BanII
BbvCI BglII Bme1580I BmrI BpmI Bpu10I BseRI BseYI
BsgI BsiHKAI Bsp1286I BstAPI BstXI BstYI BstZ17I BtsI
EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII
HindIII MmeI MslI MspA1I NdeI PasI PfoI PspOMI
PstI PvuII SacI SfcI SmaI TaqII TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstBI BstEII Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI Tth111I XbaI
XhoI ZraI