(Linear) (Six-base) MAPSORT of: KIEE0484.seq Check: 8423 from: 1 to: 678
KIEE0484.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
Acc65I G'GTAC_C
Cuts at: 0 332 678
Size: 332 346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 678
Size: 542 136
AhdI GACnn_n'nnGTC
Cuts at: 0 244 507 678
Size: 244 263 171
Fragments arranged by size:
263 244 171
AlwNI CAG_nnn'CTG
Cuts at: 0 381 678
Size: 381 297
ApoI r'AATT_y
Cuts at: 0 206 678
Size: 206 472
BanI G'GyrC_C
Cuts at: 0 332 678
Size: 332 346
BanII G_rGCy'C
Cuts at: 0 389 678
Size: 389 289
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 678
Size: 530 148
BglII A'GATC_T
Cuts at: 0 545 678
Size: 545 133
BmgBI CAC'GTC
Cuts at: 0 21 678
Size: 21 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 403 678
Size: 346 57 275
Fragments arranged by size:
346 275 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 344 374 678
Size: 344 30 304
Fragments arranged by size:
344 304 30
BseYI C'CCAG_C
Cuts at: 0 611 678
Size: 611 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 678
Size: 463 215
Bsp1286I G_dGCh'C
Cuts at: 0 389 678
Size: 389 289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 524 678
Size: 524 154
BsrFI r'CCGG_y
Cuts at: 0 593 678
Size: 593 85
BsrGI T'GTAC_A
Cuts at: 0 461 678
Size: 461 217
BstAPI GCAn_nnn'nTGC
Cuts at: 0 621 678
Size: 621 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 678
Size: 585 93
BstYI r'GATC_y
Cuts at: 0 545 600 678
Size: 545 55 78
Fragments arranged by size:
545 78 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 26 678
Size: 26 652
BtsI GCAGTG_nn'
Cuts at: 0 446 678
Size: 446 232
EarI CTCTTCn'nnn_
Cuts at: 0 101 678
Size: 101 577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 403 542 678
Size: 346 57 139 136
Fragments arranged by size:
346 139 136 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 148 678
Size: 148 530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 344 376 493 525 530 562
Size: 230 32 82 32 117 32 5 32
Cuts at: 562 678
Size: 116
Fragments arranged by size:
230 117 116 82 32 32 32 32
5
KpnI G_GTAC'C
Cuts at: 0 336 678
Size: 336 342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 678
Size: 602 76
NaeI GCC'GGC
Cuts at: 0 595 678
Size: 595 83
NdeI CA'TA_TG
Cuts at: 0 41 678
Size: 41 637
NgoMIV G'CCGG_C
Cuts at: 0 593 678
Size: 593 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 678
Size: 381 297
PstI C_TGCA'G
Cuts at: 0 441 678
Size: 441 237
SexAI A'CCwGG_T
Cuts at: 0 328 678
Size: 328 350
SfcI C'TryA_G
Cuts at: 0 437 678
Size: 437 241
SspI AAT'ATT
Cuts at: 0 166 678
Size: 166 512
TatI w'GTAC_w
Cuts at: 0 461 678
Size: 461 217
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI ApoI BanI BanII BbsI
BglII BmgBI BpmI BsaXI BseYI BsgI Bsp1286I BspMI
BsrFI BsrGI BstAPI BstXI BstYI BtgZI BtsI EarI
Eco57MI FalI Hin4I KpnI MmeI NaeI NdeI NgoMIV
PflMI PstI SexAI SfcI SspI TatI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MscI
MslI MspA1I NarI NcoI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI