(Linear) (Six-base) MAPSORT of: KIEE0484.seq Check: 8423 from: 1 to: 678 KIEE0484.orf With 182 enzymes: SgfI * April 24, 2009 13:15 .. Acc65I G'GTAC_C Cuts at: 0 332 678 Size: 332 346 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 542 678 Size: 542 136 AhdI GACnn_n'nnGTC Cuts at: 0 244 507 678 Size: 244 263 171 Fragments arranged by size: 263 244 171 AlwNI CAG_nnn'CTG Cuts at: 0 381 678 Size: 381 297 ApoI r'AATT_y Cuts at: 0 206 678 Size: 206 472 BanI G'GyrC_C Cuts at: 0 332 678 Size: 332 346 BanII G_rGCy'C Cuts at: 0 389 678 Size: 389 289 BbsI GAAGACnn'nnnn_ Cuts at: 0 530 678 Size: 530 148 BglII A'GATC_T Cuts at: 0 545 678 Size: 545 133 BmgBI CAC'GTC Cuts at: 0 21 678 Size: 21 657 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 403 678 Size: 346 57 275 Fragments arranged by size: 346 275 57 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 344 374 678 Size: 344 30 304 Fragments arranged by size: 344 304 30 BseYI C'CCAG_C Cuts at: 0 611 678 Size: 611 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 463 678 Size: 463 215 Bsp1286I G_dGCh'C Cuts at: 0 389 678 Size: 389 289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 524 678 Size: 524 154 BsrFI r'CCGG_y Cuts at: 0 593 678 Size: 593 85 BsrGI T'GTAC_A Cuts at: 0 461 678 Size: 461 217 BstAPI GCAn_nnn'nTGC Cuts at: 0 621 678 Size: 621 57 BstXI CCAn_nnnn'nTGG Cuts at: 0 585 678 Size: 585 93 BstYI r'GATC_y Cuts at: 0 545 600 678 Size: 545 55 78 Fragments arranged by size: 545 78 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 26 678 Size: 26 652 BtsI GCAGTG_nn' Cuts at: 0 446 678 Size: 446 232 EarI CTCTTCn'nnn_ Cuts at: 0 101 678 Size: 101 577 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 403 542 678 Size: 346 57 139 136 Fragments arranged by size: 346 139 136 57 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 148 678 Size: 148 530 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 230 262 344 376 493 525 530 562 Size: 230 32 82 32 117 32 5 32 Cuts at: 562 678 Size: 116 Fragments arranged by size: 230 117 116 82 32 32 32 32 5 KpnI G_GTAC'C Cuts at: 0 336 678 Size: 336 342 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 602 678 Size: 602 76 NaeI GCC'GGC Cuts at: 0 595 678 Size: 595 83 NdeI CA'TA_TG Cuts at: 0 41 678 Size: 41 637 NgoMIV G'CCGG_C Cuts at: 0 593 678 Size: 593 85 PflMI CCAn_nnn'nTGG Cuts at: 0 381 678 Size: 381 297 PstI C_TGCA'G Cuts at: 0 441 678 Size: 441 237 SexAI A'CCwGG_T Cuts at: 0 328 678 Size: 328 350 SfcI C'TryA_G Cuts at: 0 437 678 Size: 437 241 SspI AAT'ATT Cuts at: 0 166 678 Size: 166 512 TatI w'GTAC_w Cuts at: 0 461 678 Size: 461 217 Enzymes that do cut: Acc65I AcuI AhdI AlwNI ApoI BanI BanII BbsI BglII BmgBI BpmI BsaXI BseYI BsgI Bsp1286I BspMI BsrFI BsrGI BstAPI BstXI BstYI BtgZI BtsI EarI Eco57MI FalI Hin4I KpnI MmeI NaeI NdeI NgoMIV PflMI PstI SexAI SfcI SspI TatI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MscI MslI MspA1I NarI NcoI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI