Restriction map for pF1KE0484
 (Linear) (Six-base) MAPSORT of: KIEE0484.seq  Check: 8423  from: 1  to: 678



KIEE0484.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

Acc65I G'GTAC_C

Cuts at:      0     332     678 
   Size:        332     346
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     678 
   Size:        542     136
 
AhdI GACnn_n'nnGTC

Cuts at:      0     244     507     678 
   Size:        244     263     171

  Fragments arranged by size: 

                 263     244     171
 
AlwNI CAG_nnn'CTG

Cuts at:      0     381     678 
   Size:        381     297
 
ApoI r'AATT_y

Cuts at:      0     206     678 
   Size:        206     472
 
BanI G'GyrC_C

Cuts at:      0     332     678 
   Size:        332     346
 
BanII G_rGCy'C

Cuts at:      0     389     678 
   Size:        389     289
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     530     678 
   Size:        530     148
 
BglII A'GATC_T

Cuts at:      0     545     678 
   Size:        545     133
 
BmgBI CAC'GTC

Cuts at:      0      21     678 
   Size:         21     657
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     403     678 
   Size:        346      57     275

  Fragments arranged by size: 

                 346     275      57
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     344     374     678 
   Size:        344      30     304

  Fragments arranged by size: 

                 344     304      30
 
BseYI C'CCAG_C

Cuts at:      0     611     678 
   Size:        611      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     678 
   Size:        463     215
 
Bsp1286I G_dGCh'C

Cuts at:      0     389     678 
   Size:        389     289
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     524     678 
   Size:        524     154
 
BsrFI r'CCGG_y

Cuts at:      0     593     678 
   Size:        593      85
 
BsrGI T'GTAC_A

Cuts at:      0     461     678 
   Size:        461     217
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     621     678 
   Size:        621      57
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     585     678 
   Size:        585      93
 
BstYI r'GATC_y

Cuts at:      0     545     600     678 
   Size:        545      55      78

  Fragments arranged by size: 

                 545      78      55
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      26     678 
   Size:         26     652
 
BtsI GCAGTG_nn'

Cuts at:      0     446     678 
   Size:        446     232
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     678 
   Size:        101     577
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     403     542     678 
   Size:        346      57     139     136

  Fragments arranged by size: 

                 346     139     136      57
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     148     678 
   Size:        148     530
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     230     262     344     376     493     525     530     562 
   Size:        230      32      82      32     117      32       5      32

Cuts at:    562     678 
   Size:        116

  Fragments arranged by size: 

                 230     117     116      82      32      32      32      32
                   5
 
KpnI G_GTAC'C

Cuts at:      0     336     678 
   Size:        336     342
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     602     678 
   Size:        602      76
 
NaeI GCC'GGC

Cuts at:      0     595     678 
   Size:        595      83
 
NdeI CA'TA_TG

Cuts at:      0      41     678 
   Size:         41     637
 
NgoMIV G'CCGG_C

Cuts at:      0     593     678 
   Size:        593      85
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     381     678 
   Size:        381     297
 
PstI C_TGCA'G

Cuts at:      0     441     678 
   Size:        441     237
 
SexAI A'CCwGG_T

Cuts at:      0     328     678 
   Size:        328     350
 
SfcI C'TryA_G

Cuts at:      0     437     678 
   Size:        437     241
 
SspI AAT'ATT

Cuts at:      0     166     678 
   Size:        166     512
 
TatI w'GTAC_w

Cuts at:      0     461     678 
   Size:        461     217
 

 Enzymes that do cut:

   Acc65I     AcuI     AhdI    AlwNI     ApoI     BanI    BanII     BbsI
    BglII    BmgBI     BpmI    BsaXI    BseYI     BsgI Bsp1286I    BspMI
    BsrFI    BsrGI   BstAPI    BstXI    BstYI    BtgZI     BtsI     EarI
  Eco57MI     FalI    Hin4I     KpnI     MmeI     NaeI     NdeI   NgoMIV
    PflMI     PstI    SexAI     SfcI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
     MslI   MspA1I     NarI     NcoI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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