Restriction map for
pF1KE0488
(Linear) (Six-base) MAPSORT of: KIEE0488.seq Check: 9418 from: 1 to: 1107
KIEE0488.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
Acc65I G'GTAC_C
Cuts at: 0 461 1107
Size: 461 646
AfeI AGC'GCT
Cuts at: 0 406 1107
Size: 406 701
AflIII A'CryG_T
Cuts at: 0 314 803 1107
Size: 314 489 304
Fragments arranged by size:
489 314 304
AhdI GACnn_n'nnGTC
Cuts at: 0 35 1107
Size: 35 1072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 832 1107
Size: 832 275
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 566 598 1107
Size: 566 32 509
Fragments arranged by size:
566 509 32
AlwNI CAG_nnn'CTG
Cuts at: 0 375 728 1107
Size: 375 353 379
Fragments arranged by size:
379 375 353
AvaI C'yCGr_G
Cuts at: 0 24 85 493 539 1107
Size: 24 61 408 46 568
Fragments arranged by size:
568 408 61 46 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 733 766 1107
Size: 733 33 341
Fragments arranged by size:
733 341 33
BanI G'GyrC_C
Cuts at: 0 9 461 1107
Size: 9 452 646
Fragments arranged by size:
646 452 9
BanII G_rGCy'C
Cuts at: 0 826 932 1107
Size: 826 106 175
Fragments arranged by size:
826 175 106
BbeI G_GCGC'C
Cuts at: 0 13 1107
Size: 13 1094
BbsI GAAGACnn'nnnn_
Cuts at: 0 492 1107
Size: 492 615
BlpI GC'TnA_GC
Cuts at: 0 820 1107
Size: 820 287
Bme1580I G_kGCm'C
Cuts at: 0 742 1107
Size: 742 365
BmrI ACTGGGnnnn_n'
Cuts at: 0 53 927 1107
Size: 53 874 180
Fragments arranged by size:
874 180 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1107
Size: 123 984
BsaI GGTCTCn'nnnn_
Cuts at: 0 601 1107
Size: 601 506
BsaAI yAC'GTr
Cuts at: 0 231 315 1107
Size: 231 84 792
Fragments arranged by size:
792 231 84
BsaHI Gr'CG_yC
Cuts at: 0 10 1107
Size: 10 1097
BseYI C'CCAG_C
Cuts at: 0 670 938 1107
Size: 670 268 169
Fragments arranged by size:
670 268 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 1107
Size: 330 777
BsiEI CG_ry'CG
Cuts at: 0 1067 1107
Size: 1067 40
BsiHKAI G_wGCw'C
Cuts at: 0 848 1107
Size: 848 259
BsmBI CGTCTCn'nnnn_
Cuts at: 0 264 1107
Size: 264 843
Bsp1286I G_dGCh'C
Cuts at: 0 742 826 848 932 1107
Size: 742 84 22 84 175
Fragments arranged by size:
742 175 84 84 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 395 524 1107
Size: 395 129 583
Fragments arranged by size:
583 395 129
BsrFI r'CCGG_y
Cuts at: 0 142 458 473 1107
Size: 142 316 15 634
Fragments arranged by size:
634 316 142 15
BstAPI GCAn_nnn'nTGC
Cuts at: 0 875 1107
Size: 875 232
Bsu36I CC'TnA_GG
Cuts at: 0 124 1107
Size: 124 983
BtgI C'CryG_G
Cuts at: 0 742 1018 1107
Size: 742 276 89
Fragments arranged by size:
742 276 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 1107
Size: 120 987
EaeI y'GGCC_r
Cuts at: 0 1064 1107
Size: 1064 43
EagI C'GGCC_G
Cuts at: 0 1064 1107
Size: 1064 43
EarI CTCTTCn'nnn_
Cuts at: 0 214 1107
Size: 214 893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1107
Size: 123 984
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 1107
Size: 277 830
EcoO109I rG'GnC_Cy
Cuts at: 0 19 360 1107
Size: 19 341 747
Fragments arranged by size:
747 341 19
HaeII r_GCGC'y
Cuts at: 0 13 408 954 1107
Size: 13 395 546 153
Fragments arranged by size:
546 395 153 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 1107
Size: 21 32 1054
Fragments arranged by size:
1054 32 21
HincII GTy'rAC
Cuts at: 0 267 1107
Size: 267 840
HindIII A'AGCT_T
Cuts at: 0 620 1107
Size: 620 487
KasI G'GCGC_C
Cuts at: 0 9 1107
Size: 9 1098
KpnI G_GTAC'C
Cuts at: 0 465 1107
Size: 465 642
MluI A'CGCG_T
Cuts at: 0 803 1107
Size: 803 304
MslI CAynn'nnrTG
Cuts at: 0 187 814 1107
Size: 187 627 293
Fragments arranged by size:
627 293 187
MspA1I CmG'CkG
Cuts at: 0 178 674 1063 1107
Size: 178 496 389 44
Fragments arranged by size:
496 389 178 44
NaeI GCC'GGC
Cuts at: 0 475 1107
Size: 475 632
NarI GG'CG_CC
Cuts at: 0 10 1107
Size: 10 1097
NgoMIV G'CCGG_C
Cuts at: 0 473 1107
Size: 473 634
NspI r_CATG'y
Cuts at: 0 985 1107
Size: 985 122
PasI CC'CwG_GG
Cuts at: 0 826 1107
Size: 826 281
PfoI T'CCnGG_A
Cuts at: 0 1098 1107
Size: 1098 9
PmlI CAC'GTG
Cuts at: 0 231 1107
Size: 231 876
PpuMI rG'GwC_Cy
Cuts at: 0 19 1107
Size: 19 1088
PspXI vC'TCGA_Gb
Cuts at: 0 85 1107
Size: 85 1022
PstI C_TGCA'G
Cuts at: 0 305 392 637 1107
Size: 305 87 245 470
Fragments arranged by size:
470 305 245 87
SanDI GG'GwC_CC
Cuts at: 0 19 1107
Size: 19 1088
SbfI CC_TGCA'GG
Cuts at: 0 392 1107
Size: 392 715
SfcI C'TryA_G
Cuts at: 0 301 388 633 729 1107
Size: 301 87 245 96 378
Fragments arranged by size:
378 301 245 96 87
SfoI GGC'GCC
Cuts at: 0 11 1107
Size: 11 1096
SmaI CCC'GGG
Cuts at: 0 541 1107
Size: 541 566
SmlI C'TyrA_G
Cuts at: 0 85 1107
Size: 85 1022
SphI G_CATG'C
Cuts at: 0 985 1107
Size: 985 122
StyI C'CwwG_G
Cuts at: 0 14 1107
Size: 14 1093
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 364 1107
Size: 364 743
TatI w'GTAC_w
Cuts at: 0 317 1107
Size: 317 790
XhoI C'TCGA_G
Cuts at: 0 85 1107
Size: 85 1022
XmaI C'CCGG_G
Cuts at: 0 539 1107
Size: 539 568
Enzymes that do cut:
Acc65I AfeI AflIII AhdI AlfI AloI AlwNI AvaI
BaeI BanI BanII BbeI BbsI BlpI Bme1580I BmrI
BpmI BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BstAPI Bsu36I BtgI BtgZI
EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII KasI KpnI MluI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PfoI PmlI PpuMI PspXI
PstI SanDI SbfI SfcI SfoI SmaI SmlI SphI
StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AleI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HpaI MfeI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XmnI ZraI