Restriction map for
pF1KE0490
(Linear) (Six-base) MAPSORT of: KIEE0490.seq Check: 4696 from: 1 to: 1221
KIEE0490.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 212 478 1083 1221
Size: 212 266 605 138
Fragments arranged by size:
605 266 212 138
AatII G_ACGT'C
Cuts at: 0 620 1221
Size: 620 601
AfeI AGC'GCT
Cuts at: 0 235 1221
Size: 235 986
AflIII A'CryG_T
Cuts at: 0 323 1221
Size: 323 898
ApaI G_GGCC'C
Cuts at: 0 1121 1221
Size: 1121 100
ApoI r'AATT_y
Cuts at: 0 1216 1221
Size: 1216 5
AvaI C'yCGr_G
Cuts at: 0 82 707 1221
Size: 82 625 514
Fragments arranged by size:
625 514 82
AvrII C'CTAG_G
Cuts at: 0 399 1221
Size: 399 822
BamHI G'GATC_C
Cuts at: 0 30 1221
Size: 30 1191
BanI G'GyrC_C
Cuts at: 0 334 466 862 1135 1171 1221
Size: 334 132 396 273 36 50
Fragments arranged by size:
396 334 273 132 50 36
BanII G_rGCy'C
Cuts at: 0 1121 1221
Size: 1121 100
BbeI G_GCGC'C
Cuts at: 0 1139 1175 1221
Size: 1139 36 46
Fragments arranged by size:
1139 46 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 1195 1221
Size: 1195 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 41 75 1221
Size: 41 34 1146
Fragments arranged by size:
1146 41 34
BglI GCCn_nnn'nGGC
Cuts at: 0 139 1125 1169 1221
Size: 139 986 44 52
Fragments arranged by size:
986 139 52 44
BglII A'GATC_T
Cuts at: 0 191 1221
Size: 191 1030
BlpI GC'TnA_GC
Cuts at: 0 289 718 1201 1221
Size: 289 429 483 20
Fragments arranged by size:
483 429 289 20
Bme1580I G_kGCm'C
Cuts at: 0 266 865 993 1121 1221
Size: 266 599 128 128 100
Fragments arranged by size:
599 266 128 128 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 826 993 1129 1221
Size: 565 261 167 136 92
Fragments arranged by size:
565 261 167 136 92
Bpu10I CC'TnA_GC
Cuts at: 0 137 379 1221
Size: 137 242 842
Fragments arranged by size:
842 242 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1003 1221
Size: 724 279 218
Fragments arranged by size:
724 279 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 1018 1221
Size: 1018 203
BsaAI yAC'GTr
Cuts at: 0 324 639 1221
Size: 324 315 582
Fragments arranged by size:
582 324 315
BsaHI Gr'CG_yC
Cuts at: 0 617 1055 1136 1172 1221
Size: 617 438 81 36 49
Fragments arranged by size:
617 438 81 49 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 643 673 1132 1162 1221
Size: 643 30 459 30 59
Fragments arranged by size:
643 459 59 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 419 1221
Size: 286 133 802
Fragments arranged by size:
802 286 133
BseYI C'CCAG_C
Cuts at: 0 64 1221
Size: 64 1157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1221
Size: 1069 152
BsiEI CG_ry'CG
Cuts at: 0 50 1221
Size: 50 1171
BsmBI CGTCTCn'nnnn_
Cuts at: 0 75 1221
Size: 75 1146
Bsp1286I G_dGCh'C
Cuts at: 0 266 865 993 1121 1221
Size: 266 599 128 128 100
Fragments arranged by size:
599 266 128 128 100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 478 1083 1221
Size: 212 266 605 138
Fragments arranged by size:
605 266 212 138
BsrBI CCG'CTC
Cuts at: 0 136 1154 1221
Size: 136 1018 67
Fragments arranged by size:
1018 136 67
BsrFI r'CCGG_y
Cuts at: 0 874 1221
Size: 874 347
BstAPI GCAn_nnn'nTGC
Cuts at: 0 473 789 1221
Size: 473 316 432
Fragments arranged by size:
473 432 316
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 1221
Size: 102 1119
BstYI r'GATC_y
Cuts at: 0 30 191 1221
Size: 30 161 1030
Fragments arranged by size:
1030 161 30
BtgI C'CryG_G
Cuts at: 0 101 866 1140 1221
Size: 101 765 274 81
Fragments arranged by size:
765 274 101 81
BtsI GCAGTG_nn'
Cuts at: 0 95 1221
Size: 95 1126
DrdI GACnn_nn'nnGTC
Cuts at: 0 616 1221
Size: 616 605
EaeI y'GGCC_r
Cuts at: 0 797 1221
Size: 797 424
EarI CTCTTCn'nnn_
Cuts at: 0 259 304 691 697 994 1221
Size: 259 45 387 6 297 227
Fragments arranged by size:
387 297 259 227 45 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 826 993 1129 1221
Size: 565 261 167 136 92
Fragments arranged by size:
565 261 167 136 92
EcoRI G'AATT_C
Cuts at: 0 1216 1221
Size: 1216 5
FspI TGC'GCA
Cuts at: 0 247 1221
Size: 247 974
FspAI rTGC'GCAy
Cuts at: 0 247 1221
Size: 247 974
HaeII r_GCGC'y
Cuts at: 0 237 1139 1175 1221
Size: 237 902 36 46
Fragments arranged by size:
902 237 46 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 1221
Size: 22 32 1167
Fragments arranged by size:
1167 32 22
KasI G'GCGC_C
Cuts at: 0 1135 1171 1221
Size: 1135 36 50
Fragments arranged by size:
1135 50 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1221
Size: 404 817
MscI TGG'CCA
Cuts at: 0 799 1221
Size: 799 422
MslI CAynn'nnrTG
Cuts at: 0 100 898 1221
Size: 100 798 323
Fragments arranged by size:
798 323 100
MspA1I CmG'CkG
Cuts at: 0 218 455 986 1221
Size: 218 237 531 235
Fragments arranged by size:
531 237 235 218
NaeI GCC'GGC
Cuts at: 0 876 1221
Size: 876 345
NarI GG'CG_CC
Cuts at: 0 1136 1172 1221
Size: 1136 36 49
Fragments arranged by size:
1136 49 36
NcoI C'CATG_G
Cuts at: 0 101 1221
Size: 101 1120
NgoMIV G'CCGG_C
Cuts at: 0 874 1221
Size: 874 347
NspI r_CATG'y
Cuts at: 0 185 201 792 1221
Size: 185 16 591 429
Fragments arranged by size:
591 429 185 16
PfoI T'CCnGG_A
Cuts at: 0 803 1221
Size: 803 418
PspOMI G'GGCC_C
Cuts at: 0 1117 1221
Size: 1117 104
PstI C_TGCA'G
Cuts at: 0 536 1221
Size: 536 685
PvuII CAG'CTG
Cuts at: 0 218 455 1221
Size: 218 237 766
Fragments arranged by size:
766 237 218
SapI GCTCTTCn'nnn_
Cuts at: 0 994 1221
Size: 994 227
ScaI AGT'ACT
Cuts at: 0 319 1221
Size: 319 902
SfcI C'TryA_G
Cuts at: 0 213 532 726 1221
Size: 213 319 194 495
Fragments arranged by size:
495 319 213 194
SfoI GGC'GCC
Cuts at: 0 1137 1173 1221
Size: 1137 36 48
Fragments arranged by size:
1137 48 36
SmaI CCC'GGG
Cuts at: 0 709 1221
Size: 709 512
SmlI C'TyrA_G
Cuts at: 0 739 1018 1221
Size: 739 279 203
Fragments arranged by size:
739 279 203
SphI G_CATG'C
Cuts at: 0 792 1221
Size: 792 429
StuI AGG'CCT
Cuts at: 0 358 586 1221
Size: 358 228 635
Fragments arranged by size:
635 358 228
StyI C'CwwG_G
Cuts at: 0 101 359 399 1221
Size: 101 258 40 822
Fragments arranged by size:
822 258 101 40
TatI w'GTAC_w
Cuts at: 0 317 326 1088 1221
Size: 317 9 762 133
Fragments arranged by size:
762 317 133 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1132 1164 1221
Size: 1132 32 57
Fragments arranged by size:
1132 57 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1165 1221
Size: 1165 56
XmaI C'CCGG_G
Cuts at: 0 707 1221
Size: 707 514
ZraI GAC'GTC
Cuts at: 0 618 1221
Size: 618 603
Enzymes that do cut:
AarI AatII AfeI AflIII ApaI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BglI BglII
BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI
BsrBI BsrFI BstAPI BstXI BstYI BtgI BtsI DrdI
EaeI EarI Eco57MI EcoRI FspI FspAI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PfoI PspOMI PstI PvuII SapI ScaI
SfcI SfoI SmaI SmlI SphI StuI StyI TatI
TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI BaeI
BbvCI BciVI BclI BfrBI BmgBI BmrI BmtI BplI
BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI HincII HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmnI