Restriction map for
pF1KE0491
(Linear) (Six-base) MAPSORT of: KIEE0491.seq Check: 6696 from: 1 to: 969
KIEE0491.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 853 969
Size: 45 808 116
Fragments arranged by size:
808 116 45
AflIII A'CryG_T
Cuts at: 0 596 969
Size: 596 373
ApaI G_GGCC'C
Cuts at: 0 552 969
Size: 552 417
ApaLI G'TGCA_C
Cuts at: 0 592 969
Size: 592 377
ApoI r'AATT_y
Cuts at: 0 213 969
Size: 213 756
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 888 921 969
Size: 888 33 48
Fragments arranged by size:
888 48 33
BanII G_rGCy'C
Cuts at: 0 552 737 969
Size: 552 185 232
Fragments arranged by size:
552 232 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 405 439 969
Size: 405 34 530
Fragments arranged by size:
530 405 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 916 969
Size: 916 53
BclI T'GATC_A
Cuts at: 0 240 969
Size: 240 729
BglI GCCn_nnn'nGGC
Cuts at: 0 304 969
Size: 304 665
BglII A'GATC_T
Cuts at: 0 800 969
Size: 800 169
BlpI GC'TnA_GC
Cuts at: 0 817 969
Size: 817 152
Bme1580I G_kGCm'C
Cuts at: 0 552 596 969
Size: 552 44 373
Fragments arranged by size:
552 373 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 294 637 699 729 874 969
Size: 90 204 343 62 30 145 95
Fragments arranged by size:
343 204 145 95 90 62 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 479 969
Size: 479 490
BsaHI Gr'CG_yC
Cuts at: 0 752 969
Size: 752 217
BsaWI w'CCGG_w
Cuts at: 0 181 969
Size: 181 788
BseYI C'CCAG_C
Cuts at: 0 679 969
Size: 679 290
BsiEI CG_ry'CG
Cuts at: 0 609 969
Size: 609 360
BsiHKAI G_wGCw'C
Cuts at: 0 596 969
Size: 596 373
Bsp1286I G_dGCh'C
Cuts at: 0 552 596 737 969
Size: 552 44 141 232
Fragments arranged by size:
552 232 141 44
BspEI T'CCGG_A
Cuts at: 0 181 969
Size: 181 788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 205 424 969
Size: 205 219 545
Fragments arranged by size:
545 219 205
BsrBI CCG'CTC
Cuts at: 0 713 969
Size: 713 256
BsrFI r'CCGG_y
Cuts at: 0 412 608 969
Size: 412 196 361
Fragments arranged by size:
412 361 196
BsrGI T'GTAC_A
Cuts at: 0 464 809 969
Size: 464 345 160
Fragments arranged by size:
464 345 160
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 969
Size: 81 888
BstYI r'GATC_y
Cuts at: 0 800 969
Size: 800 169
BtgI C'CryG_G
Cuts at: 0 629 641 728 761 794 969
Size: 629 12 87 33 33 175
Fragments arranged by size:
629 175 87 33 33 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 348 549 969
Size: 348 201 420
Fragments arranged by size:
420 348 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 90 294 637 699 729 853 874
Size: 45 45 204 343 62 30 124 21
Cuts at: 874 969
Size: 95
Fragments arranged by size:
343 204 124 95 62 45 45 30
21
EcoO109I rG'GnC_Cy
Cuts at: 0 305 548 549 825 969
Size: 305 243 1 276 144
Fragments arranged by size:
305 276 243 144 1
HaeII r_GCGC'y
Cuts at: 0 705 969
Size: 705 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 969
Size: 383 32 554
Fragments arranged by size:
554 383 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 962 969
Size: 893 69 7
Fragments arranged by size:
893 69 7
MslI CAynn'nnrTG
Cuts at: 0 79 460 969
Size: 79 381 509
Fragments arranged by size:
509 381 79
MspA1I CmG'CkG
Cuts at: 0 621 969
Size: 621 348
NaeI GCC'GGC
Cuts at: 0 414 969
Size: 414 555
NgoMIV G'CCGG_C
Cuts at: 0 412 969
Size: 412 557
NspI r_CATG'y
Cuts at: 0 600 969
Size: 600 369
PciI A'CATG_T
Cuts at: 0 596 969
Size: 596 373
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 910 942 969
Size: 910 32 27
Fragments arranged by size:
910 32 27
PpuMI rG'GwC_Cy
Cuts at: 0 825 969
Size: 825 144
PspOMI G'GGCC_C
Cuts at: 0 548 969
Size: 548 421
PstI C_TGCA'G
Cuts at: 0 435 969
Size: 435 534
PvuII CAG'CTG
Cuts at: 0 621 969
Size: 621 348
SanDI GG'GwC_CC
Cuts at: 0 825 969
Size: 825 144
SfcI C'TryA_G
Cuts at: 0 431 969
Size: 431 538
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 304 969
Size: 304 665
SspI AAT'ATT
Cuts at: 0 286 969
Size: 286 683
StuI AGG'CCT
Cuts at: 0 658 969
Size: 658 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 340 969
Size: 340 629
TatI w'GTAC_w
Cuts at: 0 464 809 969
Size: 464 345 160
Fragments arranged by size:
464 345 160
Enzymes that do cut:
AcuI AflIII ApaI ApaLI ApoI BaeI BanII BcgI
BciVI BclI BglI BglII BlpI Bme1580I BpmI BsaI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BstXI BstYI BtgI BtgZI Eco57MI
EcoO109I HaeII Hin4I MmeI MslI MspA1I NaeI NgoMIV
NspI PciI PpiI PpuMI PspOMI PstI PvuII SanDI
SfcI SfiI SspI StuI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BbsI BbvCI BfrBI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BspHI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StyI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI