Restriction map for
pF1KE0503
(Linear) (Six-base) MAPSORT of: KIEE0503.seq Check: 8723 from: 1 to: 660
KIEE0503.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 660
Size: 328 332
AfeI AGC'GCT
Cuts at: 0 7 124 660
Size: 7 117 536
Fragments arranged by size:
536 117 7
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 635 660
Size: 635 25
AlwNI CAG_nnn'CTG
Cuts at: 0 72 90 246 285 516 660
Size: 72 18 156 39 231 144
Fragments arranged by size:
231 156 144 72 39 18
BamHI G'GATC_C
Cuts at: 0 386 660
Size: 386 274
BanI G'GyrC_C
Cuts at: 0 16 40 172 660
Size: 16 24 132 488
Fragments arranged by size:
488 132 24 16
BbeI G_GCGC'C
Cuts at: 0 20 660
Size: 20 640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 196 660
Size: 162 34 464
Fragments arranged by size:
464 162 34
BglI GCCn_nnn'nGGC
Cuts at: 0 313 660
Size: 313 347
BlpI GC'TnA_GC
Cuts at: 0 142 353 660
Size: 142 211 307
Fragments arranged by size:
307 211 142
Bme1580I G_kGCm'C
Cuts at: 0 45 660
Size: 45 615
BmrI ACTGGGnnnn_n'
Cuts at: 0 621 660
Size: 621 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 448 487 660
Size: 223 225 39 173
Fragments arranged by size:
225 223 173 39
Bpu10I CC'TnA_GC
Cuts at: 0 274 660
Size: 274 386
BsaAI yAC'GTr
Cuts at: 0 495 660
Size: 495 165
BsaHI Gr'CG_yC
Cuts at: 0 17 660
Size: 17 643
BsaWI w'CCGG_w
Cuts at: 0 383 437 548 660
Size: 383 54 111 112
Fragments arranged by size:
383 112 111 54
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 409 660
Size: 40 369 251
Fragments arranged by size:
369 251 40
BseYI C'CCAG_C
Cuts at: 0 106 308 572 660
Size: 106 202 264 88
Fragments arranged by size:
264 202 106 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 660
Size: 211 449
BsiHKAI G_wGCw'C
Cuts at: 0 275 383 463 660
Size: 275 108 80 197
Fragments arranged by size:
275 197 108 80
Bsp1286I G_dGCh'C
Cuts at: 0 45 275 383 463 660
Size: 45 230 108 80 197
Fragments arranged by size:
230 197 108 80 45
BspEI T'CCGG_A
Cuts at: 0 548 660
Size: 548 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 542 660
Size: 341 201 118
Fragments arranged by size:
341 201 118
BsrFI r'CCGG_y
Cuts at: 0 320 660
Size: 320 340
BsrGI T'GTAC_A
Cuts at: 0 233 660
Size: 233 427
BstAPI GCAn_nnn'nTGC
Cuts at: 0 270 285 660
Size: 270 15 375
Fragments arranged by size:
375 270 15
BstBI TT'CG_AA
Cuts at: 0 182 660
Size: 182 478
BstYI r'GATC_y
Cuts at: 0 206 386 660
Size: 206 180 274
Fragments arranged by size:
274 206 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 421 660
Size: 421 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 660
Size: 49 611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 328 448 487 660
Size: 223 105 120 39 173
Fragments arranged by size:
223 173 120 105 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 660
Size: 94 566
EcoO109I rG'GnC_Cy
Cuts at: 0 604 660
Size: 604 56
HaeII r_GCGC'y
Cuts at: 0 9 20 126 163 246 286 660
Size: 9 11 106 37 83 40 374
Fragments arranged by size:
374 106 83 40 37 11 9
KasI G'GCGC_C
Cuts at: 0 16 660
Size: 16 644
MspA1I CmG'CkG
Cuts at: 0 69 402 660
Size: 69 333 258
Fragments arranged by size:
333 258 69
NaeI GCC'GGC
Cuts at: 0 322 660
Size: 322 338
NarI GG'CG_CC
Cuts at: 0 17 660
Size: 17 643
NgoMIV G'CCGG_C
Cuts at: 0 320 660
Size: 320 340
PasI CC'CwG_GG
Cuts at: 0 591 660
Size: 591 69
PfoI T'CCnGG_A
Cuts at: 0 425 660
Size: 425 235
PpuMI rG'GwC_Cy
Cuts at: 0 604 660
Size: 604 56
PstI C_TGCA'G
Cuts at: 0 527 560 660
Size: 527 33 100
Fragments arranged by size:
527 100 33
PvuII CAG'CTG
Cuts at: 0 69 660
Size: 69 591
SfcI C'TryA_G
Cuts at: 0 253 523 556 660
Size: 253 270 33 104
Fragments arranged by size:
270 253 104 33
SfoI GGC'GCC
Cuts at: 0 18 660
Size: 18 642
StuI AGG'CCT
Cuts at: 0 316 660
Size: 316 344
StyI C'CwwG_G
Cuts at: 0 20 660
Size: 20 640
TatI w'GTAC_w
Cuts at: 0 233 660
Size: 233 427
Enzymes that do cut:
AcuI AfeI AlfI AlwNI BamHI BanI BbeI BcgI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI
BsrFI BsrGI BstAPI BstBI BstYI BtgZI EciI Eco57MI
EcoNI EcoO109I HaeII KasI MspA1I NaeI NarI NgoMIV
PasI PfoI PpuMI PstI PvuII SfcI SfoI StuI
StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanII BbsI BbvCI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BpuEI BsaI
BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrDI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI