Restriction map for
pF1KE0506
(Linear) (Six-base) MAPSORT of: KIEE0506.seq Check: 7893 from: 1 to: 987
KIEE0506.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 987
Size: 108 879
AflIII A'CryG_T
Cuts at: 0 239 326 987
Size: 239 87 661
Fragments arranged by size:
661 239 87
AleI CACnn'nnGTG
Cuts at: 0 254 987
Size: 254 733
AlwNI CAG_nnn'CTG
Cuts at: 0 387 816 987
Size: 387 429 171
Fragments arranged by size:
429 387 171
ApaI G_GGCC'C
Cuts at: 0 509 987
Size: 509 478
AvaI C'yCGr_G
Cuts at: 0 774 925 987
Size: 774 151 62
Fragments arranged by size:
774 151 62
BanI G'GyrC_C
Cuts at: 0 921 987
Size: 921 66
BanII G_rGCy'C
Cuts at: 0 509 538 987
Size: 509 29 449
Fragments arranged by size:
509 449 29
BbvCI CC'TCA_GC
Cuts at: 0 215 987
Size: 215 772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 930 964 987
Size: 930 34 23
Fragments arranged by size:
930 34 23
BlpI GC'TnA_GC
Cuts at: 0 37 987
Size: 37 950
Bme1580I G_kGCm'C
Cuts at: 0 509 926 987
Size: 509 417 61
Fragments arranged by size:
509 417 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 743 987
Size: 743 244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 663 987
Size: 663 324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 987
Size: 943 44
Bpu10I CC'TnA_GC
Cuts at: 0 215 987
Size: 215 772
BsaI GGTCTCn'nnnn_
Cuts at: 0 61 130 987
Size: 61 69 857
Fragments arranged by size:
857 69 61
BsaAI yAC'GTr
Cuts at: 0 327 987
Size: 327 660
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 987
Size: 437 30 520
Fragments arranged by size:
520 437 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 762 987
Size: 184 578 225
Fragments arranged by size:
578 225 184
BseYI C'CCAG_C
Cuts at: 0 274 508 664 987
Size: 274 234 156 323
Fragments arranged by size:
323 274 234 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 987
Size: 286 701
BsiHKAI G_wGCw'C
Cuts at: 0 323 987
Size: 323 664
BsmBI CGTCTCn'nnnn_
Cuts at: 0 251 568 987
Size: 251 317 419
Fragments arranged by size:
419 317 251
Bsp1286I G_dGCh'C
Cuts at: 0 323 509 538 926 987
Size: 323 186 29 388 61
Fragments arranged by size:
388 323 186 61 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 844 987
Size: 844 143
BsrBI CCG'CTC
Cuts at: 0 78 987
Size: 78 909
BsrDI GCAATG_nn'
Cuts at: 0 486 987
Size: 486 501
BsrFI r'CCGG_y
Cuts at: 0 110 987
Size: 110 877
BstAPI GCAn_nnn'nTGC
Cuts at: 0 113 850 987
Size: 113 737 137
Fragments arranged by size:
737 137 113
BstEII G'GTnAC_C
Cuts at: 0 279 987
Size: 279 708
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 720 987
Size: 228 492 267
Fragments arranged by size:
492 267 228
Bsu36I CC'TnA_GG
Cuts at: 0 143 987
Size: 143 844
BtgI C'CryG_G
Cuts at: 0 392 515 987
Size: 392 123 472
Fragments arranged by size:
472 392 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 288 573 987
Size: 288 285 414
Fragments arranged by size:
414 288 285
BtsI GCAGTG_nn'
Cuts at: 0 269 987
Size: 269 718
DraI TTT'AAA
Cuts at: 0 180 987
Size: 180 807
DraIII CAC_nnn'GTG
Cuts at: 0 27 327 987
Size: 27 300 660
Fragments arranged by size:
660 300 27
EaeI y'GGCC_r
Cuts at: 0 314 987
Size: 314 673
EarI CTCTTCn'nnn_
Cuts at: 0 97 619 987
Size: 97 522 368
Fragments arranged by size:
522 368 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 458 987
Size: 458 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 943 987
Size: 108 835 44
Fragments arranged by size:
835 108 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 987
Size: 697 290
EcoO109I rG'GnC_Cy
Cuts at: 0 384 856 954 987
Size: 384 472 98 33
Fragments arranged by size:
472 384 98 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 108 987
Size: 108 879
FspI TGC'GCA
Cuts at: 0 799 987
Size: 799 188
FspAI rTGC'GCAy
Cuts at: 0 799 987
Size: 799 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 840 872 987
Size: 840 32 115
Fragments arranged by size:
840 115 32
MslI CAynn'nnrTG
Cuts at: 0 157 254 370 718 987
Size: 157 97 116 348 269
Fragments arranged by size:
348 269 157 116 97
MspA1I CmG'CkG
Cuts at: 0 254 871 963 987
Size: 254 617 92 24
Fragments arranged by size:
617 254 92 24
PasI CC'CwG_GG
Cuts at: 0 816 987
Size: 816 171
PflMI CCAn_nnn'nTGG
Cuts at: 0 408 918 987
Size: 408 510 69
Fragments arranged by size:
510 408 69
PmlI CAC'GTG
Cuts at: 0 327 987
Size: 327 660
PpuMI rG'GwC_Cy
Cuts at: 0 384 856 954 987
Size: 384 472 98 33
Fragments arranged by size:
472 384 98 33
PspOMI G'GGCC_C
Cuts at: 0 505 987
Size: 505 482
SapI GCTCTTCn'nnn_
Cuts at: 0 97 987
Size: 97 890
StuI AGG'CCT
Cuts at: 0 977 987
Size: 977 10
StyI C'CwwG_G
Cuts at: 0 635 987
Size: 635 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 288 987
Size: 85 203 699
Fragments arranged by size:
699 203 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 507 539 987
Size: 437 32 38 32 448
Fragments arranged by size:
448 437 38 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 547 742 987
Size: 547 195 245
Fragments arranged by size:
547 245 195
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI AvaI BanI BanII
BbvCI BcgI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI FspI FspAI Hin4I
MslI MspA1I PasI PflMI PmlI PpuMI PspOMI SapI
StuI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BpuEI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI
BstBI BstYI BstZ17I ClaI CspCI DrdI EagI EcoICRI
EcoRI EcoRV FseI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI ZraI