Restriction map for
pF1KE0510
(Linear) (Six-base) MAPSORT of: KIEE0510.seq Check: 8004 from: 1 to: 705
KIEE0510.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
Acc65I G'GTAC_C
Cuts at: 0 278 705
Size: 278 427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 231 300 705
Size: 204 27 69 405
Fragments arranged by size:
405 204 69 27
AfeI AGC'GCT
Cuts at: 0 188 705
Size: 188 517
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 705
Size: 148 32 525
Fragments arranged by size:
525 148 32
AlwNI CAG_nnn'CTG
Cuts at: 0 288 523 705
Size: 288 235 182
Fragments arranged by size:
288 235 182
ApaI G_GGCC'C
Cuts at: 0 148 705
Size: 148 557
ApoI r'AATT_y
Cuts at: 0 580 638 705
Size: 580 58 67
Fragments arranged by size:
580 67 58
AvaI C'yCGr_G
Cuts at: 0 148 209 705
Size: 148 61 496
Fragments arranged by size:
496 148 61
BanI G'GyrC_C
Cuts at: 0 195 278 705
Size: 195 83 427
Fragments arranged by size:
427 195 83
BanII G_rGCy'C
Cuts at: 0 148 705
Size: 148 557
BglI GCCn_nnn'nGGC
Cuts at: 0 143 705
Size: 143 562
BlpI GC'TnA_GC
Cuts at: 0 76 705
Size: 76 629
Bme1580I G_kGCm'C
Cuts at: 0 148 705
Size: 148 557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 705
Size: 253 452
Bpu10I CC'TnA_GC
Cuts at: 0 403 705
Size: 403 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 63 332 705
Size: 63 269 373
Fragments arranged by size:
373 269 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 705
Size: 148 30 527
Fragments arranged by size:
527 148 30
BsmI GAATG_Cn'
Cuts at: 0 357 705
Size: 357 348
Bsp1286I G_dGCh'C
Cuts at: 0 148 705
Size: 148 557
BtgI C'CryG_G
Cuts at: 0 139 432 705
Size: 139 293 273
Fragments arranged by size:
293 273 139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 98 705
Size: 98 607
BtsI GCAGTG_nn'
Cuts at: 0 606 705
Size: 606 99
EaeI y'GGCC_r
Cuts at: 0 46 705
Size: 46 659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 231 253 300 705
Size: 204 27 22 47 405
Fragments arranged by size:
405 204 47 27 22
EcoO109I rG'GnC_Cy
Cuts at: 0 144 145 705
Size: 144 1 560
Fragments arranged by size:
560 144 1
HaeII r_GCGC'y
Cuts at: 0 190 705
Size: 190 515
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 538 570 705
Size: 76 32 430 32 135
Fragments arranged by size:
430 135 76 32 32
KpnI G_GTAC'C
Cuts at: 0 282 705
Size: 282 423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 705
Size: 103 602
MspA1I CmG'CkG
Cuts at: 0 119 705
Size: 119 586
NcoI C'CATG_G
Cuts at: 0 139 432 705
Size: 139 293 273
Fragments arranged by size:
293 273 139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 705
Size: 148 32 525
Fragments arranged by size:
525 148 32
PspOMI G'GGCC_C
Cuts at: 0 144 705
Size: 144 561
PstI C_TGCA'G
Cuts at: 0 194 293 705
Size: 194 99 412
Fragments arranged by size:
412 194 99
SfcI C'TryA_G
Cuts at: 0 190 289 705
Size: 190 99 416
Fragments arranged by size:
416 190 99
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 143 705
Size: 143 562
SmaI CCC'GGG
Cuts at: 0 150 705
Size: 150 555
SmlI C'TyrA_G
Cuts at: 0 209 705
Size: 209 496
StyI C'CwwG_G
Cuts at: 0 139 432 632 705
Size: 139 293 200 73
Fragments arranged by size:
293 200 139 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 320 705
Size: 320 385
XhoI C'TCGA_G
Cuts at: 0 209 705
Size: 209 496
XmaI C'CCGG_G
Cuts at: 0 148 705
Size: 148 557
Enzymes that do cut:
Acc65I AcuI AfeI AloI AlwNI ApaI ApoI AvaI
BanI BanII BglI BlpI Bme1580I BpmI Bpu10I BsaI
BsaXI BsmI Bsp1286I BtgI BtgZI BtsI EaeI Eco57MI
EcoO109I HaeII Hin4I KpnI MmeI MspA1I NcoI PpiI
PspOMI PstI SfcI SfiI SmaI SmlI StyI TaqII
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XmnI ZraI