(Linear) (Six-base) MAPSORT of: KIEE0512.seq Check: 7102 from: 1 to: 855 KIEE0512.orf With 182 enzymes: SgfI * April 24, 2009 13:15 .. AccI GT'mk_AC Cuts at: 0 35 855 Size: 35 820 AflIII A'CryG_T Cuts at: 0 92 590 855 Size: 92 498 265 Fragments arranged by size: 498 265 92 AlwNI CAG_nnn'CTG Cuts at: 0 699 717 855 Size: 699 18 138 Fragments arranged by size: 699 138 18 ApaI G_GGCC'C Cuts at: 0 19 855 Size: 19 836 AvaI C'yCGr_G Cuts at: 0 526 855 Size: 526 329 BamHI G'GATC_C Cuts at: 0 504 855 Size: 504 351 BanII G_rGCy'C Cuts at: 0 19 362 404 527 554 821 855 Size: 19 343 42 123 27 267 34 Fragments arranged by size: 343 267 123 42 34 27 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 111 855 Size: 111 744 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 507 541 855 Size: 507 34 314 Fragments arranged by size: 507 314 34 Bme1580I G_kGCm'C Cuts at: 0 19 775 855 Size: 19 756 80 Fragments arranged by size: 756 80 19 BmgBI CAC'GTC Cuts at: 0 95 789 855 Size: 95 694 66 Fragments arranged by size: 694 95 66 BmrI ACTGGGnnnn_n' Cuts at: 0 177 399 855 Size: 177 222 456 Fragments arranged by size: 456 222 177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 855 Size: 484 371 Bpu10I CC'TnA_GC Cuts at: 0 847 855 Size: 847 8 BsaWI w'CCGG_w Cuts at: 0 83 317 650 855 Size: 83 234 333 205 Fragments arranged by size: 333 234 205 83 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 391 421 855 Size: 391 30 434 Fragments arranged by size: 434 391 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 349 723 855 Size: 349 374 132 Fragments arranged by size: 374 349 132 BsiHKAI G_wGCw'C Cuts at: 0 527 855 Size: 527 328 Bsp1286I G_dGCh'C Cuts at: 0 19 362 404 527 554 775 821 855 Size: 19 343 42 123 27 221 46 34 Fragments arranged by size: 343 221 123 46 42 34 27 19 BspEI T'CCGG_A Cuts at: 0 83 317 855 Size: 83 234 538 Fragments arranged by size: 538 234 83 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 242 788 855 Size: 242 546 67 Fragments arranged by size: 546 242 67 BstYI r'GATC_y Cuts at: 0 504 855 Size: 504 351 Bsu36I CC'TnA_GG Cuts at: 0 632 855 Size: 632 223 BtgI C'CryG_G Cuts at: 0 227 458 855 Size: 227 231 397 Fragments arranged by size: 397 231 227 BtsI GCAGTG_nn' Cuts at: 0 622 855 Size: 622 233 DraIII CAC_nnn'GTG Cuts at: 0 770 855 Size: 770 85 EaeI y'GGCC_r Cuts at: 0 62 719 855 Size: 62 657 136 Fragments arranged by size: 657 136 62 EcoICRI GAG'CTC Cuts at: 0 525 855 Size: 525 330 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 855 Size: 484 371 EcoNI CCTnn'n_nnAGG Cuts at: 0 376 855 Size: 376 479 EcoO109I rG'GnC_Cy Cuts at: 0 100 381 561 714 855 Size: 100 281 180 153 141 Fragments arranged by size: 281 180 153 141 100 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 96 855 Size: 96 759 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 239 417 855 Size: 239 178 438 Fragments arranged by size: 438 239 178 MscI TGG'CCA Cuts at: 0 64 721 855 Size: 64 657 134 Fragments arranged by size: 657 134 64 MspA1I CmG'CkG Cuts at: 0 307 699 855 Size: 307 392 156 Fragments arranged by size: 392 307 156 NspI r_CATG'y Cuts at: 0 181 594 855 Size: 181 413 261 Fragments arranged by size: 413 261 181 PasI CC'CwG_GG Cuts at: 0 731 855 Size: 731 124 PciI A'CATG_T Cuts at: 0 590 855 Size: 590 265 PflMI CCAn_nnn'nTGG Cuts at: 0 156 855 Size: 156 699 PfoI T'CCnGG_A Cuts at: 0 107 482 500 855 Size: 107 375 18 355 Fragments arranged by size: 375 355 107 18 PpuMI rG'GwC_Cy Cuts at: 0 100 381 561 714 855 Size: 100 281 180 153 141 Fragments arranged by size: 281 180 153 141 100 PspOMI G'GGCC_C Cuts at: 0 15 855 Size: 15 840 PvuII CAG'CTG Cuts at: 0 699 855 Size: 699 156 SacI G_AGCT'C Cuts at: 0 527 855 Size: 527 328 ScaI AGT'ACT Cuts at: 0 832 855 Size: 832 23 SexAI A'CCwGG_T Cuts at: 0 596 855 Size: 596 259 SphI G_CATG'C Cuts at: 0 181 855 Size: 181 674 StuI AGG'CCT Cuts at: 0 454 855 Size: 454 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 344 723 855 Size: 344 379 132 Fragments arranged by size: 379 344 132 TatI w'GTAC_w Cuts at: 0 587 830 855 Size: 587 243 25 Fragments arranged by size: 587 243 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 84 116 391 423 855 Size: 84 32 275 32 432 Fragments arranged by size: 432 275 84 32 32 Tth111I GACn'n_nGTC Cuts at: 0 115 163 559 855 Size: 115 48 396 296 Fragments arranged by size: 396 296 115 48 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 338 855 Size: 338 517 Enzymes that do cut: AccI AflIII AlwNI ApaI AvaI BamHI BanII BbsI BcgI Bme1580I BmgBI BmrI BpmI Bpu10I BsaWI BsaXI BsgI BsiHKAI Bsp1286I BspEI BspMI BstYI Bsu36I BtgI BtsI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI MmeI MscI MspA1I NspI PasI PciI PflMI PfoI PpuMI PspOMI PvuII SacI ScaI SexAI SphI StuI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StyI SwaI XbaI XhoI XmaI XmnI ZraI