(Linear) (Six-base) MAPSORT of: KIEE0515.seq  Check: 5618  from: 1  to: 651



KIEE0515.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AflIII A'CryG_T

Cuts at:      0     293     651 
   Size:        293     358
 
AlwNI CAG_nnn'CTG

Cuts at:      0     457     651 
   Size:        457     194
 
ApoI r'AATT_y

Cuts at:      0     508     651 
   Size:        508     143
 
AvaI C'yCGr_G

Cuts at:      0      78     651 
   Size:         78     573
 
BanI G'GyrC_C

Cuts at:      0      50     651 
   Size:         50     601
 
BbeI G_GCGC'C

Cuts at:      0      54     651 
   Size:         54     597
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     605     651 
   Size:        605      46
 
BglI GCCn_nnn'nGGC

Cuts at:      0     310     651 
   Size:        310     341
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      50     651 
   Size:         50     601
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     651 
   Size:        434     217
 
Bpu10I CC'TnA_GC

Cuts at:      0     458     651 
   Size:        458     193
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     651 
   Size:        323     328
 
BsaHI Gr'CG_yC

Cuts at:      0      51     651 
   Size:         51     600
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     359     389     651 
   Size:        359      30     262

  Fragments arranged by size: 

                 359     262      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     182     651 
   Size:        182     469
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     419     651 
   Size:        210     209     232

  Fragments arranged by size: 

                 232     210     209
 
BspHI T'CATG_A

Cuts at:      0     158     651 
   Size:        158     493
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      94     257     651 
   Size:         94     163     394

  Fragments arranged by size: 

                 394     163      94
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     354     651 
   Size:        354     297
 
EarI CTCTTCn'nnn_

Cuts at:      0     470     651 
   Size:        470     181
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     651 
   Size:        434     217
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     535     651 
   Size:        535     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0      83     311     426     528     651 
   Size:         83     228     115     102     123

  Fragments arranged by size: 

                 228     123     115     102      83
 
EcoRI G'AATT_C

Cuts at:      0     508     651 
   Size:        508     143
 
HaeII r_GCGC'y

Cuts at:      0      54     144     651 
   Size:         54      90     507

  Fragments arranged by size: 

                 507      90      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     545     577     651 
   Size:        545      32      74

  Fragments arranged by size: 

                 545      74      32
 
KasI G'GCGC_C

Cuts at:      0      50     651 
   Size:         50     601
 
NarI GG'CG_CC

Cuts at:      0      51     651 
   Size:         51     600
 
NspI r_CATG'y

Cuts at:      0     297     651 
   Size:        297     354
 
PasI CC'CwG_GG

Cuts at:      0     531     651 
   Size:        531     120
 
PciI A'CATG_T

Cuts at:      0     293     651 
   Size:        293     358
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     150     651 
   Size:        150     501
 
PfoI T'CCnGG_A

Cuts at:      0     494     587     651 
   Size:        494      93      64

  Fragments arranged by size: 

                 494      93      64
 
PpuMI rG'GwC_Cy

Cuts at:      0      83     651 
   Size:         83     568
 
SapI GCTCTTCn'nnn_

Cuts at:      0     470     651 
   Size:        470     181
 
SfcI C'TryA_G

Cuts at:      0     184     315     651 
   Size:        184     131     336

  Fragments arranged by size: 

                 336     184     131
 
SfoI GGC'GCC

Cuts at:      0      52     651 
   Size:         52     599
 
SmaI CCC'GGG

Cuts at:      0      80     651 
   Size:         80     571
 
SmlI C'TyrA_G

Cuts at:      0     338     651 
   Size:        338     313
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     482     651 
   Size:        482     169
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     351     651 
   Size:        351     300
 
XmaI C'CCGG_G

Cuts at:      0      78     651 
   Size:         78     573
 
XmnI GAAnn'nnTTC

Cuts at:      0     508     651 
   Size:        508     143
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     AvaI     BanI     BbeI     BbsI     BglI
     BmrI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI     BsgI
    BspHI    BspMI    BstXI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    HaeII    Hin4I     KasI     NarI     NspI     PasI     PciI    PflMI
     PfoI    PpuMI     SapI     SfcI     SfoI     SmaI     SmlI    TaqII
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaWI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     ZraI