Restriction map for
pF1KE0519
(Linear) (Six-base) MAPSORT of: KIEE0519.seq Check: 6085 from: 1 to: 576
KIEE0519.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 576
Size: 499 32 45
Fragments arranged by size:
499 45 32
AlwNI CAG_nnn'CTG
Cuts at: 0 519 576
Size: 519 57
ApaI G_GGCC'C
Cuts at: 0 17 59 576
Size: 17 42 517
Fragments arranged by size:
517 42 17
BanII G_rGCy'C
Cuts at: 0 17 59 576
Size: 17 42 517
Fragments arranged by size:
517 42 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 319 576
Size: 319 257
BclI T'GATC_A
Cuts at: 0 551 576
Size: 551 25
BglI GCCn_nnn'nGGC
Cuts at: 0 161 576
Size: 161 415
Bme1580I G_kGCm'C
Cuts at: 0 17 59 576
Size: 17 42 517
Fragments arranged by size:
517 42 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 53 299 492 576
Size: 53 246 193 84
Fragments arranged by size:
246 193 84 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 461 576
Size: 27 434 115
Fragments arranged by size:
434 115 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 576
Size: 314 262
BsaI GGTCTCn'nnnn_
Cuts at: 0 196 576
Size: 196 380
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 311 464 576
Size: 311 153 112
Fragments arranged by size:
311 153 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 576
Size: 68 508
Bsp1286I G_dGCh'C
Cuts at: 0 17 59 576
Size: 17 42 517
Fragments arranged by size:
517 42 17
BsrBI CCG'CTC
Cuts at: 0 89 576
Size: 89 487
BsrDI GCAATG_nn'
Cuts at: 0 78 576
Size: 78 498
BstAPI GCAn_nnn'nTGC
Cuts at: 0 122 576
Size: 122 454
DrdI GACnn_nn'nnGTC
Cuts at: 0 224 576
Size: 224 352
EaeI y'GGCC_r
Cuts at: 0 162 178 576
Size: 162 16 398
Fragments arranged by size:
398 162 16
EarI CTCTTCn'nnn_
Cuts at: 0 175 535 576
Size: 175 360 41
Fragments arranged by size:
360 175 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 461 576
Size: 27 434 115
Fragments arranged by size:
434 115 27
EcoO109I rG'GnC_Cy
Cuts at: 0 13 14 55 300 576
Size: 13 1 41 245 276
Fragments arranged by size:
276 245 41 13 1
HaeII r_GCGC'y
Cuts at: 0 80 576
Size: 80 496
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 245 277 306 338 576
Size: 245 32 29 32 238
Fragments arranged by size:
245 238 32 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 576
Size: 179 397
MscI TGG'CCA
Cuts at: 0 164 576
Size: 164 412
MspA1I CmG'CkG
Cuts at: 0 481 576
Size: 481 95
PasI CC'CwG_GG
Cuts at: 0 487 576
Size: 487 89
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 576
Size: 504 72
PpuMI rG'GwC_Cy
Cuts at: 0 300 576
Size: 300 276
PspOMI G'GGCC_C
Cuts at: 0 13 55 576
Size: 13 42 521
Fragments arranged by size:
521 42 13
PvuII CAG'CTG
Cuts at: 0 481 576
Size: 481 95
SmlI C'TyrA_G
Cuts at: 0 329 576
Size: 329 247
StyI C'CwwG_G
Cuts at: 0 137 576
Size: 137 439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 515 547 576
Size: 515 32 29
Fragments arranged by size:
515 32 29
Tth111I GACn'n_nGTC
Cuts at: 0 259 576
Size: 259 317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 406 576
Size: 406 170
Enzymes that do cut:
AloI AlwNI ApaI BanII BbsI BclI BglI Bme1580I
BmrI BpmI BpuEI BsaI BseRI BsgI Bsp1286I BsrBI
BsrDI BstAPI DrdI EaeI EarI Eco57MI EcoO109I HaeII
Hin4I MmeI MscI MspA1I PasI PflMI PpuMI PspOMI
PvuII SmlI StyI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI XbaI XhoI XmaI XmnI ZraI