(Linear) (Six-base) MAPSORT of: KIEE0523.seq  Check: 1235  from: 1  to: 684



KIEE0523.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     684 
   Size:        566     118
 
AflIII A'CryG_T

Cuts at:      0     393     684 
   Size:        393     291
 
AgeI A'CCGG_T

Cuts at:      0     413     684 
   Size:        413     271
 
ApoI r'AATT_y

Cuts at:      0     235     684 
   Size:        235     449
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     263     684 
   Size:        263     421
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     684 
   Size:        670      14
 
BsaAI yAC'GTr

Cuts at:      0     394     684 
   Size:        394     290
 
BsaWI w'CCGG_w

Cuts at:      0     413     684 
   Size:        413     271
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     182     684 
   Size:        182     502
 
BsrFI r'CCGG_y

Cuts at:      0     413     684 
   Size:        413     271
 
Bsu36I CC'TnA_GG

Cuts at:      0     215     684 
   Size:        215     469
 
BtgI C'CryG_G

Cuts at:      0     158     684 
   Size:        158     526
 
DraI TTT'AAA

Cuts at:      0     233     684 
   Size:        233     451
 
DraIII CAC_nnn'GTG

Cuts at:      0     340     475     684 
   Size:        340     135     209

  Fragments arranged by size: 

                 340     209     135
 
EarI CTCTTCn'nnn_

Cuts at:      0     662     684 
   Size:        662      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     670     684 
   Size:        566     104      14

  Fragments arranged by size: 

                 566     104      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     208     684 
   Size:        208     476
 
MslI CAynn'nnrTG

Cuts at:      0     584     684 
   Size:        584     100
 
PasI CC'CwG_GG

Cuts at:      0      47     684 
   Size:         47     637
 
PmlI CAC'GTG

Cuts at:      0     394     684 
   Size:        394     290
 
PpuMI rG'GwC_Cy

Cuts at:      0     208     684 
   Size:        208     476
 
PstI C_TGCA'G

Cuts at:      0     179     684 
   Size:        179     505
 
SfcI C'TryA_G

Cuts at:      0     128     175     684 
   Size:        128      47     509

  Fragments arranged by size: 

                 509     128      47
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     621     653     684 
   Size:        621      32      31

  Fragments arranged by size: 

                 621      32      31
 
XmnI GAAnn'nnTTC

Cuts at:      0     671     684 
   Size:        671      13
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     ApoI     BbsI     BpmI    BsaAI    BsaWI
    BspMI    BsrFI   Bsu36I     BtgI     DraI   DraIII     EarI  Eco57MI
 EcoO109I     MslI     PasI     PmlI    PpuMI     PstI     SfcI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI